MBCQ
Based on 1 Customer Validation
MBCQ is a potent and selective cGMP-specific phosphodiesterase (PDE V; PDE5) inhibitor with an IC50 of 19 nM. MBCQ lacks inhibitory activity toward other PDE isozymes (all IC50s>100 μM). MBCQ dilates coronary arteries via specific inhibition of cGMP-PDE.
For research use only. We do not sell to patients.
- Purity: 99.80%
- CAS No.: 150450-53-6
- Formula: C16H12ClN3O2
- Molecular Weight:313.74
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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PDE V 19 nM (IC50) |
PDE Ⅰ >100 μM (IC50) |
PDE Ⅱ >100 μM (IC50) |
PDE Ⅲ >100 μM (IC50) |
PDE Ⅳ >100 μM (IC50) |
MBCQ (compound 3d) induces relaxation of isolated porcine coronary arteries precontracted with prostaglandin F2α (PGF2α; EC50=190 nM)[1].
MBCQ (0.01-10 μM; 10 min) inhibits Carbachol (10 μM)-induced contractions in a concentration-dependent manner in rat ileal smooth muscle[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 150450-53-6
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Appearance Solid
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Molecular Weight 313.74
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Formula C16H12ClN3O2
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Color Off-white to yellow
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SMILES
ClC1=CC2=C(N=CN=C2NCC3=CC4=C(C=C3)OCO4)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (318.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (398 KB)
- English - EN (398 KB)
- Français - FR (398 KB)
- Deutsch - DE (398 KB)
- Norwegian - NO (398 KB)
- Español - ES (398 KB)
- Swedish - SV (398 KB)
- Italian - IT (398 KB)
- Portuguese - PT (398 KB)
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Handling Instructions (2659 KB)
References
[1]. Y Takase, et al. Cyclic GMP phosphodiesterase inhibitors. 2. Requirement of 6-substitution of quinazoline derivatives for potent and selective inhibitory activity. J Med Chem. 1994 Jun 24;37(13):2106-11. [Content Brief]
[2]. Jeffery D MacPherson, et al. Inhibition of phosphodiesterase 5 selectively reverses nitrate tolerance in the venous circulation J Pharmacol Exp Ther. 2006 Apr;317(1):188-95. [Content Brief]
[3]. Takeharu Kaneda, et al. Lack of cyclic nucleotide regulation of MBCQ-induced relaxation of rat ileal smooth muscle. J Smooth Muscle Res. 2003 Jun;39(3):47-54. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.1874 mL | 15.9368 mL | 31.8735 mL | 79.6838 mL |
| 5 mM | 0.6375 mL | 3.1874 mL | 6.3747 mL | 15.9368 mL | |
| 10 mM | 0.3187 mL | 1.5937 mL | 3.1874 mL | 7.9684 mL | |
| 15 mM | 0.2125 mL | 1.0625 mL | 2.1249 mL | 5.3123 mL | |
| 20 mM | 0.1594 mL | 0.7968 mL | 1.5937 mL | 3.9842 mL | |
| 25 mM | 0.1275 mL | 0.6375 mL | 1.2749 mL | 3.1874 mL | |
| 30 mM | 0.1062 mL | 0.5312 mL | 1.0625 mL | 2.6561 mL | |
| 40 mM | 0.0797 mL | 0.3984 mL | 0.7968 mL | 1.9921 mL | |
| 50 mM | 0.0637 mL | 0.3187 mL | 0.6375 mL | 1.5937 mL | |
| 60 mM | 0.0531 mL | 0.2656 mL | 0.5312 mL | 1.3281 mL | |
| 80 mM | 0.0398 mL | 0.1992 mL | 0.3984 mL | 0.9960 mL | |
| 100 mM | 0.0319 mL | 0.1594 mL | 0.3187 mL | 0.7968 mL |
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