Pancracine
Pancracine is an alkaloid. Pancracine upregulates p27, phosphorylated p38 MAPK and phosphorylated p53, while downregulating phosphorylated Akt and phosphorylated Rb. Pancracine induces G1 cell cycle arrest and Apoptosis in cells. Pancracine inhibits the replication of pseudotyped HIV-1 and Dengue virus. Pancracine can be used in research related to lung adenocarcinoma, acute lymphoblastic leukemia, cutaneous epidermoid carcinoma, human immunodeficiency virus infection and dengue virus infection.
For research use only. We do not sell to patients.
- CAS No.: 21416-14-8
- Formula: C16H17NO4
- Molecular Weight:287.31
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
|
HIV-1 |
Pancracine (1-50 μM; up to 96 h) inhibits the proliferation of A549, MCF-7, HepG2 and A2780 cells in a concentration-dependent manner, and 20 μM Pancracine completely arrests the proliferation of A549 cells for up to 96 h[1].
Pancracine (2.5-20 μM; 24-72 h (A549); 24-48 h (MOLT-4)) exerts significant antiproliferative effects on A549 and MOLT-4 cells at concentrations ≥2.5 μM, and MOLT-4 cells are more sensitive to Pancracine-induced viability loss than A549 cells[1].
Pancracine (0.5-10 μM; 24 h) exhibits selective cytotoxicity against the epidermoid cancer cell line A431[2].
Pancracine (0.05-200 μM) exhibits cytotoxicity against THP-1 acute monocytic leukemia cells, with a CC50 value of 25.93 μM[2].
Pancracine (2.5-20 μM; 24-48 h) induces G1 phase arrest in A549 cells at 5 μM for 24 h, and shifts to G2 phase arrest at 20 μM for 48 h; meanwhile, it reduces the proportion of S phase cells at higher concentrations and with longer incubation times[1].
Pancracine (10-20 μM; 24-72 h) inhibits the proliferation of A549 cells by regulating the Akt/p27/pRb and p38 MAPK/ERK signaling pathways; when the concentration is ≥10 μM and the treatment lasts for 72 h, increased phosphorylation level of p38 MAPK, decreased phosphorylation levels of ERK and pRb, as well as increased p27 level and decreased phosphorylation level of Akt are observed[1].
Pancracine (2.5-10 μM; 24-48 h) induces G1 phase arrest and increases the sub-G1 apoptotic cell population in MOLT-4 cells. Significant G1 phase shift is observed at 2.5 μM after 24 h and at 5 μM after 48 h, and the sub-G1 apoptotic cells increase in a dose-dependent manner[1].
Pancracine (5-20 μM) significantly increases the activities of caspase-3/7, -8, and -9 in MOLT-4 cells at concentrations ≥10 μM after 24 h of treatment, but does not induce caspase activation in A549 cells[1].
Pancracine (2.5-20 μM; 24 h) induces dose-dependent apoptosis in MOLT-4 cells after 24 hours, with the total proportion of apoptotic cells reaching 48% (14% early apoptosis and 34% late apoptosis) following treatment with 20 μM Pancracine[1].
Pancracine (0.5-200 μM; 72 h) potently inhibits infection by pseudotyped HIV-1GFP in THP-1 acute monocytic leukemia cells, with an EC50 of 18.51 μM, and achieves complete inhibition at a concentration of 100 μM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:human A549 lung adenocarcinoma, MCF-7 breast adenocarcinoma, HepG2 hepatocellular carcinoma, A2780 ovarian carcinoma cells
-
Concentration:1, 5, 10, 20, 50 μM
-
Incubation Time:up to 96 h
-
Result:Reduced proliferation in all tested cell lines in a concentration-dependent manner.
Halted proliferation entirely in A549 cells over the 96-h assay interval at 20 μM.
Significantly reduced A549 cell proliferation at 5 and 10 μM.
-
Cell Line:human A549 lung adenocarcinoma, MOLT-4 acute lymphoblastic leukemia cells
-
Concentration:2.5, 5, 10, 20 μM
-
Incubation Time:24-72 h (A549); 24-48 h (MOLT-4)
-
Result:Exerted significant antiproliferative effect on A549 cells at 2.5 μM after 48 and 72 h, with increasing effects at higher concentrations.
Caused slight decrease in A549 cell viability only at 10 and 20 μM.
Exerted significant antiproliferative effect on MOLT-4 cells at 2.5 μM after 24 and 48 h.
Reduced MOLT-4 viability significantly at 5 μM after 48 h and at 10 μM after 24 h.
Reduced MOLT-4 viability to ~20% at 20 μM.
-
Cell Line:human A549 lung adenocarcinoma cells
-
Concentration:2.5-20 μM (24 h); 20 μM (48 h)
-
Incubation Time:24-48 h
-
Result:Caused significant increase in A549 cells in G1 phase at 5 μM after 24 h.
Caused significant increase in G1 and G2 phase cells, with concurrent decrease in S phase cells at 20 μM after 24 h.
Caused significant increase in G2 phase cells, with reduction in G1 and S phase cells at 20 μM after 48 h.
-
Cell Line:human MOLT-4 acute lymphoblastic leukemia cells
-
Concentration:2.5, 5, 10, 20 μM
-
Incubation Time:24 h
-
Result:Resulted in early apoptotic rates of 3%, 4%, 10%, and 14% at 2.5, 5, 10, and 20 μM respectively after 24 h.
Resulted in late apoptotic rates of 2%, 3%, 14%, and 34% at 2.5, 5, 10, and 20 μM respectively after 24 h.
Resulted in 48% total apoptotic cells (14% early, 34% late) at 20 μM after 24 h.
Chemical Information
-
CAS No. 21416-14-8
-
Molecular Weight 287.31
-
Formula C16H17NO4
-
SMILES
O[C@H]1C=C2[C@]3([H])C4=CC(OCO5)=C5C=C4C[N@]([C@@]2([H])C[C@@H]1O)C3
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Koutová D, et al. Pancracine, a Montanine-Type Amaryllidaceae Alkaloid, Inhibits Proliferation of A549 Lung Adenocarcinoma Cells and Induces Apoptotic Cell Death in MOLT-4 Leukemic Cells. International journal of molecular sciences. 2021 Jun 29;22(13):7014. [Content Brief]
[2]. Masi M, et al. Cytotoxicity and Antiviral Properties of Alkaloids Isolated from Pancratium maritimum. Toxins (Basel). 2022 Apr 7;14(4):262. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pancracine Related Classifications
HY-N20721 Related Classifications
Powered by Bioz