Prochloraz (Synonyms: BTS 40542)

Name: Prochloraz
Brand: MedChemExpress
SKU: HY-B0845
Rating: 4.5 (1 reviews)

Cat. No.: HY-B0845 Purity: 99.77%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Prochloraz is an imidazole antifungal. Prochloraz is as an estrogen receptor (ER) and androgen receptor (AR) antagonist and an aromatase inhibitor with IC₅₀ values of 25 μM, 4 μM and 0.3 μM, respectively. Prochloraz is able to activate the aryl hydrocarbon receptor (AhR) having an EC₅₀ of 1 μM.

For research use only. We do not sell to patients.

Prochloraz Chemical Structure

CAS No. : 67747-09-5

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
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10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
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250 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Biological Activity
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References
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Description

IC₅₀ & Target

IC50: 25 μM (Estrogen receptor), 4 μM (Androgen receptor) and 0.3 μM (Aromatase)^[2]
EC50: 1 μM (Aryl hydrocarbon receptor (AhR))^[2]

In Vitro

Prochloraz has a strong inhibition of the microsomal cytochrome P-450 dependent 14α-demethylation of lanosterol during ergosterol biosynthesis, which leads to membrane disruption and eventually to cell death^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

In vivo Prochloraz acts as an antiandrogen in the Hershberger assay by reducing weights of reproductive organs, affecting androgen-regulated gene expressions in the prostate and increasing luteinizing hormone levels. In order to investigate the developmental effects of Prochloraz, pregnant Wistar dams were dosed perinatally with 30 mg/kg Prochloraz. Results show that Prochloraz significantly reduces plasma and testicular testosterone levels in gestational day 21 male foetuses, whereas testicular progesterone was increased. Gestational length is increased by Prochloraz^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

376.67

Formula

C₁₅H₁₆Cl₃N₃O₂

CAS No.

67747-09-5

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1C=CN=C1)N(CCC)CCOC2=C(Cl)C=C(Cl)C=C2Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (663.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6548 mL	13.2742 mL	26.5484 mL
5 mM	0.5310 mL	2.6548 mL	5.3097 mL
10 mM	0.2655 mL	1.3274 mL	2.6548 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 3

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.98 mg/mL (5.26 mM); Clear solution

This protocol yields a clear solution of ≥ 1.98 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (19.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.77%

Select Batch:

Data Sheet (274 KB) SDS (537 KB)

COA (249 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. E Antignac, et al. Prochloraz as potent inhibitor of benzo[a]pyrene metabolism and mutagenic activity in rat liver fractions. Toxicol Lett. 1990 Dec;54(2-3):309-15. [Content Brief]

[2]. Anne Marie Vinggaard, et al. Prochloraz: an imidazole fungicide with multiple mechanisms of action. Int J Androl. 2006 Feb;29(1):186-92. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6548 mL	13.2742 mL	26.5484 mL	66.3711 mL
	5 mM	0.5310 mL	2.6548 mL	5.3097 mL	13.2742 mL
	10 mM	0.2655 mL	1.3274 mL	2.6548 mL	6.6371 mL
	15 mM	0.1770 mL	0.8849 mL	1.7699 mL	4.4247 mL
	20 mM	0.1327 mL	0.6637 mL	1.3274 mL	3.3186 mL
	25 mM	0.1062 mL	0.5310 mL	1.0619 mL	2.6548 mL
	30 mM	0.0885 mL	0.4425 mL	0.8849 mL	2.2124 mL
	40 mM	0.0664 mL	0.3319 mL	0.6637 mL	1.6593 mL
	50 mM	0.0531 mL	0.2655 mL	0.5310 mL	1.3274 mL
	60 mM	0.0442 mL	0.2212 mL	0.4425 mL	1.1062 mL
	80 mM	0.0332 mL	0.1659 mL	0.3319 mL	0.8296 mL
	100 mM	0.0265 mL	0.1327 mL	0.2655 mL	0.6637 mL

Prochloraz Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Prochloraz (Synonyms: BTS 40542)

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Prochloraz Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

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View All Estrogen Receptor/ERR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Prochloraz Related Antibodies

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Complete Stock Solution Preparation Table

Prochloraz Related Classifications

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