Stigmasta-3,5-dien-7-one
Based on 1 Customer Validation
Stigmasta-3,5-dien-7-one is a steroid compound that can be isolated from Harrisonia abyssinica. Stigmasta-3,5-dien-7-one blocks the NF-κB signaling pathway via down-regulation of phospho-p38 mitogen-activated protein kinase and phosphorylation and degradation of inhibitor of NF-κB α. Stigmasta-3,5-dien-7-one reduces LPS (HY-D1056)-induced nitric oxide, PGE2, and pro-inflammatory cytokine levels in macrophages. Stigmasta-3,5-dien-7-one can be used for inflammation diseases.
For research use only. We do not sell to patients.
- Purity: 99.84%
- CAS No.: 2034-72-2
- Formula: C29H46O
- Molecular Weight:410.67
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Stigmasta-3,5-dien-7-one (5-100 μg/mL; 1 h pre-incubation prior to LPS stimulation) dose-dependently inhibits LPS-induced NO production in RAW264.7 macrophage cells, with no cytotoxicity[1].
Stigmasta-3,5-dien-7-one (50 μg/mL; 1 h pre-incubation prior to LPS stimulation) significantly inhibits LPS-induced PGE2 production in RAW264.7 macrophage cells without altering basal PGE2 levels[1].
Stigmasta-3,5-dien-7-one (50 μg/mL; 1 h pre-incubation prior to LPS stimulation) significantly inhibits LPS-induced production of TNF-α, IL-1β, IL-6, iNOS, and COX-2 protein expression in RAW264.7 macrophage cells, without altering basal cytokine levels[1].
Stigmasta-3,5-dien-7-one (50 μg/mL; 1 h pre-incubation prior to LPS stimulation) inhibits LPS-induced NF-κB activation in RAW264.7 macrophage cells by blocking IκBα phosphorylation, IκBα degradation, and NF-κB nuclear translocation[1].
Stigmasta-3,5-dien-7-one (50 μg/mL; 1 h pre-incubation prior to LPS stimulation) inhibits LPS-induced p38 MAPK phosphorylation in RAW264.7 macrophage cells, while having no effect on ERK or SAPK/JNK phosphorylation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RAW264.7 macrophage cells
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Concentration:5; 10; 25; 50; 100 μg/mL
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Incubation Time:1 h pre-incubation prior to LPS stimulation
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Result:Showed no cytotoxicity at all tested concentrations.
Maintained cell viability at or near 100% across all doses, both in the presence and absence of LPS.
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Cell Line:RAW264.7 macrophage cells
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Concentration:50 μg/mL
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Incubation Time:1 h pre-incubation prior to LPS stimulation
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Result:Significantly inhibited LPS-induced PGE2 production compared to the LPS-only group.
Had no effect on basal PGE2 levels in the absence of LPS.\n
Significantly inhibited LPS-induced production of TNF-α, IL-1β, and IL-6 compared to the LPS-only group.
Had no effect on basal cytokine levels in the absence of LPS.
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Cell Line:RAW264.7 macrophage cells
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Concentration:50 μg/mL
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Incubation Time:1 h pre-incubation prior to LPS stimulation, followed by co-incubation with LPS for experimental period
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Result:Reduced LPS-induced iNOS and COX-2 protein expression compared to the LPS-only group.
Had no effect on basal iNOS or COX-2 expression in the absence of LPS.\nInhibited LPS-induced phosphorylation and degradation of IκBα compared to the LPS-only group.
Suppressed LPS-induced nuclear translocation of NF-κB compared to the LPS-only group.
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Cell Line:RAW264.7 macrophage cells
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Concentration:50 μg/mL
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Incubation Time:1 h pre-incubation prior to 15-min LPS stimulation
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Result:Suppressed LPS-induced phosphorylation of p38 MAPK compared to the LPS-only group.
Had no significant inhibitory effect on LPS-induced phosphorylation of ERK or SAPK/JNK MAPK compared to the LPS-only group.
Chemical Information
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CAS No. 2034-72-2
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Appearance Solid
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Molecular Weight 410.67
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Formula C29H46O
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Color Off-white to light yellow
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SMILES
O=C1[C@@]2([H])[C@@]3([H])[C@@]([C@@](CC3)([H])[C@H](C)CC[C@@H](CC)C(C)C)(CC[C@]2([H])[C@@]4(C(C=CCC4)=C1)C)C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Purity & Documentation
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Data Sheet (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Park SJ, et al. Anti-inflammatory Steroid from Phragmitis rhizoma Modulates LPS-Mediated Signaling Through Inhibition of NF-κB Pathway. Inflammation. 2016;39(2):727-734. [Content Brief]
[2]. Baldé AM, et al. Steroids from Harrisonia abyssinica. Planta Med. 2000;66(1):67-69. [Content Brief]
[3]. Szakiel A, et al. Triterpenoid content of berries and leaves of bilberry Vaccinium myrtillus from Finland and Poland. J Agric Food Chem. 2012;60(48):11839-11849. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Stigmasta-3,5-dien-7-one Related Classifications
HY-N12188 Related Classifications
Powered by Bioz
- Stigmasta-3,5-dien-7-one
- 2034-72-2
- NF-κB
- TNF Receptor
- Interleukin Related
- p38 MAPK
- phospho-p38 mitogen-activated protein kinase
- RAW264.7 macrophage cells
- inhibitor of NF-κB α
- NF-κB signaling pathway
- prostaglandin E2
- macrophages
- p38 mitogen-activated protein kinase
- LPS-induced inflammation
- nitric oxide
- Inhibitor
- inhibitor
- inhibit