2. NF-κB Apoptosis Immunology/Inflammation MAPK/ERK Pathway
  3. NF-κB TNF Receptor Interleukin Related p38 MAPK
  4. Stigmasta-3,5-dien-7-one

Stigmasta-3,5-dien-7-one is a steroid compound that can be isolated from Harrisonia abyssinica. Stigmasta-3,5-dien-7-one blocks the NF-κB signaling pathway via down-regulation of phospho-p38 mitogen-activated protein kinase and phosphorylation and degradation of inhibitor of NF-κB α. Stigmasta-3,5-dien-7-one reduces LPS (HY-D1056)-induced nitric oxide, PGE2, and pro-inflammatory cytokine levels in macrophages. Stigmasta-3,5-dien-7-one can be used for inflammation diseases.

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Stigmasta-3,5-dien-7-one

Stigmasta-3,5-dien-7-one Chemical Structure

CAS No. : 2034-72-2

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Stigmasta-3,5-dien-7-one Related Antibodies

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p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

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Description

Stigmasta-3,5-dien-7-one is a steroid compound that can be isolated from Harrisonia abyssinica. Stigmasta-3,5-dien-7-one blocks the NF-κB signaling pathway via down-regulation of phospho-p38 mitogen-activated protein kinase and phosphorylation and degradation of inhibitor of NF-κB α. Stigmasta-3,5-dien-7-one reduces LPS (HY-D1056)-induced nitric oxide, PGE2, and pro-inflammatory cytokine levels in macrophages. Stigmasta-3,5-dien-7-one can be used for inflammation diseases[1][2][3].

In Vitro

Stigmasta-3,5-dien-7-one (5-100 μg/mL; 1 h pre-incubation prior to LPS stimulation) dose-dependently inhibits LPS-induced NO production in RAW264.7 macrophage cells, with no cytotoxicity[1].
Stigmasta-3,5-dien-7-one (50 μg/mL; 1 h pre-incubation prior to LPS stimulation) significantly inhibits LPS-induced PGE2 production in RAW264.7 macrophage cells without altering basal PGE2 levels[1].
Stigmasta-3,5-dien-7-one (50 μg/mL; 1 h pre-incubation prior to LPS stimulation) significantly inhibits LPS-induced production of TNF-α, IL-1β, IL-6, iNOS, and COX-2 protein expression in RAW264.7 macrophage cells, without altering basal cytokine levels[1].
Stigmasta-3,5-dien-7-one (50 μg/mL; 1 h pre-incubation prior to LPS stimulation) inhibits LPS-induced NF-κB activation in RAW264.7 macrophage cells by blocking IκBα phosphorylation, IκBα degradation, and NF-κB nuclear translocation[1].
Stigmasta-3,5-dien-7-one (50 μg/mL; 1 h pre-incubation prior to LPS stimulation) inhibits LPS-induced p38 MAPK phosphorylation in RAW264.7 macrophage cells, while having no effect on ERK or SAPK/JNK phosphorylation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Stigmasta-3,5-dien-7-one Related Antibodies

Cell Viability Assay[1]

Cell Line: RAW264.7 macrophage cells
Concentration: 5; 10; 25; 50; 100 μg/mL
Incubation Time: 1 h pre-incubation prior to LPS stimulation
Result: Showed no cytotoxicity at all tested concentrations.
Maintained cell viability at or near 100% across all doses, both in the presence and absence of LPS.

ELISA Assay[1]

Cell Line: RAW264.7 macrophage cells
Concentration: 50 μg/mL
Incubation Time: 1 h pre-incubation prior to LPS stimulation
Result: Significantly inhibited LPS-induced PGE2 production compared to the LPS-only group.
Had no effect on basal PGE2 levels in the absence of LPS.\n
Significantly inhibited LPS-induced production of TNF-α, IL-1β, and IL-6 compared to the LPS-only group.
Had no effect on basal cytokine levels in the absence of LPS.

Western Blot Analysis[1]

Cell Line: RAW264.7 macrophage cells
Concentration: 50 μg/mL
Incubation Time: 1 h pre-incubation prior to LPS stimulation, followed by co-incubation with LPS for experimental period
Result: Reduced LPS-induced iNOS and COX-2 protein expression compared to the LPS-only group.
Had no effect on basal iNOS or COX-2 expression in the absence of LPS.\nInhibited LPS-induced phosphorylation and degradation of IκBα compared to the LPS-only group.
Suppressed LPS-induced nuclear translocation of NF-κB compared to the LPS-only group.

Western Blot Analysis[1]

Cell Line: RAW264.7 macrophage cells
Concentration: 50 μg/mL
Incubation Time: 1 h pre-incubation prior to 15-min LPS stimulation
Result: Suppressed LPS-induced phosphorylation of p38 MAPK compared to the LPS-only group.
Had no significant inhibitory effect on LPS-induced phosphorylation of ERK or SAPK/JNK MAPK compared to the LPS-only group.
Molecular Weight

410.67

Formula

C29H46O
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C1[C@@]2([H])[C@@]3([H])[C@@]([C@@](CC3)([H])[C@H](C)CC[C@@H](CC)C(C)C)(CC[C@]2([H])[C@@]4(C(C=CCC4)=C1)C)C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Purity & Documentation

Purity: 99.84%

SDS (251 KB)

COA (250 KB) HNMR (263 KB) RP-HPLC (140 KB) MS (74 KB)

Handling Instructions (2659 KB)
References
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Stigmasta-3,5-dien-7-one Related Classifications

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Help & FAQs
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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Stigmasta-3,5-dien-7-one2034-72-2NF-κBTNF ReceptorInterleukin Relatedp38 MAPKNuclear factor-κBNuclear factor-kappaBTumor Necrosis Factor ReceptorTNFRILphospho-p38 mitogen-activated protein kinaseRAW264.7 macrophage cellsinhibitor of NF-κB αNF-κB signaling pathwayprostaglandin E2macrophagesp38 mitogen-activated protein kinaseLPS-induced inflammationnitric oxideInhibitorinhibitorinhibit

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