Temozolomide acid
Based on 1 Customer Validation
Temozolomide acid is a carboxylic acid derivative of Temozolomide (HY-17364) with anticancer activity. Temozolomide is a DNA alkylating agent, methylating the guanine and adenine bases of DNA, causing breaks in DNA double strand, cell cycle arrest, and eventually cell death. Temozolomide acid is promising for research of glioblastoma and brain cancer.
For research use only. We do not sell to patients.
- Purity: 99.81%
- CAS No.: 113942-30-6
- Formula: C6H5N5O3
- Molecular Weight:195.14
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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FDA Approved & Pharmacopeial Drug Library
HY-L066
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FDA-Approved Anticancer Drug Library
HY-L122
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Off-patent Drug Library
HY-L141
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Drug Repurposing Compound Library Plus
HY-L035P
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FDA-Approved Drug Library Plus
HY-L022P
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FDA-Approved Drug Library Mini
HY-L022M
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Bioactive Compound Library
HY-L001
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Anti-Brain Cancer Compound Library
HY-L188
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High-Throughput Bioactive Compound Library
HY-L205
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MCE Kinase Hinge Binder Fragment Library
HY-L907
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Cell Cycle/DNA Damage Compound Library
HY-L004
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FDA-Approved Drug Library
HY-L022
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Anti-Cancer Compound Library
HY-L025
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Anti-Aging Compound Library
HY-L034
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Drug Repurposing Compound Library
HY-L035
All DNA/RNA Synthesis Isoforms
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Biological Activity
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Cell Line
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Type | Value | Description | References |
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| HEK-293T | IC50 |
>120 μM
Compound: TMZ
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Cytotoxicity against human 293T cells assessed as reduction in cell viability after 4 days by MTS assay
Cytotoxicity against human 293T cells assessed as reduction in cell viability after 4 days by MTS assay
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[PMID: 29486949] |
Temozolomide acid (0-1000 μM, 72 h) significantly decreases the GL261 cells and BL6 cells viability with cytotoxicity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:GL261 cells and BL6 cells
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Concentration:0-1000 μM
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Incubation Time:72 h
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Result:Showed cytotoxicity at 1 mM concentration with BL6 cells viability reduced to ̴ 15%.
Chemical Information
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CAS No. 113942-30-6
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Appearance Solid
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Molecular Weight 195.14
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Formula C6H5N5O3
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Color White to off-white
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SMILES
CN1N=NC2=C(N=CN2C1=O)C(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (256.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 5.1245 mL | 25.6226 mL | 51.2453 mL | 128.1131 mL |
| 5 mM | 1.0249 mL | 5.1245 mL | 10.2491 mL | 25.6226 mL | |
| 10 mM | 0.5125 mL | 2.5623 mL | 5.1245 mL | 12.8113 mL | |
| 15 mM | 0.3416 mL | 1.7082 mL | 3.4164 mL | 8.5409 mL | |
| 20 mM | 0.2562 mL | 1.2811 mL | 2.5623 mL | 6.4057 mL | |
| 25 mM | 0.2050 mL | 1.0249 mL | 2.0498 mL | 5.1245 mL | |
| 30 mM | 0.1708 mL | 0.8541 mL | 1.7082 mL | 4.2704 mL | |
| 40 mM | 0.1281 mL | 0.6406 mL | 1.2811 mL | 3.2028 mL | |
| 50 mM | 0.1025 mL | 0.5125 mL | 1.0249 mL | 2.5623 mL | |
| 60 mM | 0.0854 mL | 0.4270 mL | 0.8541 mL | 2.1352 mL | |
| 80 mM | 0.0641 mL | 0.3203 mL | 0.6406 mL | 1.6014 mL | |
| 100 mM | 0.0512 mL | 0.2562 mL | 0.5125 mL | 1.2811 mL |
Temozolomide acid Related Classifications
HY-W018326 Related Classifications
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