2. Metabolic Enzyme/Protease
  3. Phosphodiesterase (PDE)
  4. TPN729MA

TPN729MA  (Synonyms: TPN729 maleate)

Cat. No.: HY-116808B Purity: 99.45%
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TPN729MA (TPN729 maleate) is a selective phosphodiesterase type 5 (PDE5) inhibitor with an IC50 value of 2.28 nM. TPN729MA affects erectile function and it can be used for the research of erectile dysfunction.

For research use only. We do not sell to patients.

TPN729MA Chemical Structure

TPN729MA Chemical Structure

CAS No. : 1422955-52-9

Size Price Stock Quantity
1 mg USD 129 In-stock
5 mg USD 298 In-stock
10 mg USD 478 In-stock
25 mg USD 1003 In-stock
50 mg USD 1606 In-stock
100 mg USD 2570 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of TPN729MA:

TPN729MA Related Antibodies

Top Publications Citing Use of Products

View All Phosphodiesterase (PDE) Isoform Specific Products:

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PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

TPN729MA (TPN729 maleate) is a selective phosphodiesterase type 5 (PDE5) inhibitor with an IC50 value of 2.28 nM. TPN729MA affects erectile function and it can be used for the research of erectile dysfunction[1].

IC50 & Target

PDE5

2.28 nM (IC50)

PDE6

45.2 nM (IC50)

PDE1

566 nM (IC50)

PDE4

834 nM (IC50)

PDE11

6090 nM (IC50)

In Vivo

TPN729MA (1.25, 2.5 and 5.0 mg/kg; intraduodenal injection, once) increases intracavernous pressure/blood pressure (ICP/BP) with the dose of 5.0 mg/kg at all time points and with the dose of 2.5 mg/kg at 75, 90, 105, and 120 minutes time points[1].
TPN729MA (5.0 μg/kg, i.v., once) significantly increases ICP and ICP/BP of male Beagle dogs[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats with corpora cavernosa complete exposure[1]
Dosage: 1.25, 2.5 and 5.0 mg/kg
Administration: Intraduodenal (i.d.) injection; 1.25, 2.5 and 5.0 mg/kg, once
Result: Dose-dependently increased the maximum intracavernous pressure (ICP) and ICP/blood pressure (BP).
Molecular Weight

632.73

Formula

C29H40N6O8S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC(/C=C\C(O)=O)=O.CCCOC1=C(C(NC2=C3N(N=C2CCC)C)=NC3=O)C=C(C=C1)S(N(C)CCN4CCCC4)(=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (39.51 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5805 mL 7.9023 mL 15.8045 mL
5 mM 0.3161 mL 1.5805 mL 3.1609 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.95 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (3.95 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5805 mL 7.9023 mL 15.8045 mL 39.5113 mL
5 mM 0.3161 mL 1.5805 mL 3.1609 mL 7.9023 mL
10 mM 0.1580 mL 0.7902 mL 1.5805 mL 3.9511 mL
15 mM 0.1054 mL 0.5268 mL 1.0536 mL 2.6341 mL
20 mM 0.0790 mL 0.3951 mL 0.7902 mL 1.9756 mL
25 mM 0.0632 mL 0.3161 mL 0.6322 mL 1.5805 mL
30 mM 0.0527 mL 0.2634 mL 0.5268 mL 1.3170 mL
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TPN729MA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TPN729MA1422955-52-9TPN729TPN 729TPN-729Phosphodiesterase (PDE)selectiveICPICP/BPdogratInhibitorinhibitorinhibit

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