TS-002455

Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.

Dielectrophoresis-based electrical cell sorting separates suspended cells by the motion generated when polarizable cells experience a non-uniform electric field; cell trajectory depends on cell size, medium conductivity, applied AC frequency, electric-field gradient, and cell dielectric properties, so cells with different DEP responses can be routed, trapped, levitated, or released without biochemical labeling. In practical DEP sorters, the readout is the spatial redistribution of cells into different outlets, traps, or recovered fractions; reported examples include DEP field-flow fractionation of leukocytes, breast cancer cells, CD34+ cells, and blood cells, continuous-flow hMSC/osteoblast sorting, and image-based single-cell recovery after DEP manipulation.