Cat. No.: HY-N9965

2'-Fucosyllactose

(Synonyms: 2'-FL)

5 Cited Publications

Customer Review

Based on 5 publication(s) in Google Scholar

2'-Fucosyllactose (2'-FL) is an oligosaccharide that could be derived from human milk. 2'-Fucosyllactose regulates the expression of CD14, alleviates colitis and regulates the gut microbiome. 2'-Fucosyllactose stimulates T cells to increase IFN-γ production and decreases IL-6, IL-17, and TNF-α production of cytokines.

For research use only. We do not sell to patients.

Purity: 99.97%
CAS No.: 41263-94-9
Formula: C₁₈H₃₂O₁₅
Molecular Weight:488.44

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

2'-Fucosyllactose Chemical Structure

CAS No.: 41263-94-9

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Target: IFN-γ, IL-6, IL-17, and TNF-α^[1]

All TNF Receptor Isoforms :

All Interleukin Related Isoforms :

In Vitro

2'-Fucosyllactose (2'-FL; 0-12 mg/mL; 48 h) suppress cell-associated CD14 expression and to attenuate LPS (100 μg/mL) stimulated IL-8 secretion in T84 cells^[1].
2'-Fucosyllactose (2 mg/mL; 48 h; T84 and HCT8 cells) ameliorates inflammation induced by bacterial invasion. 2'-Fucosyllactose inhibits ETEC invasion and attenuated the consequent IL-8 secretion^[1].
2'-Fucosyllactose (2 mg/mL; 48 h; T84 and HCT8 cells) induces macrophage migration inhibitory factor signal pathways that suppress inflammation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

T84 cells
Concentration:

0, 2, and 4 mg/mL
Incubation Time:

48 hours
Result:

Suppressed CD14 mRNA and reduced cell-associated CD14 protein expression.

Western Blot Analysis^[1]

Cell Line:

T84 and HCT8 cellsT84 and HCT8 cells
Concentration:

2 mg/mL
Incubation Time:

48 hours
Result:

Suppressed CD14 mRNA and reduced cell-associated CD14 protein expression.
Inhibited CD14 and NF-κB induction.
Induced iκB and SOCS2 expression and STAT3 phosphorylation.

In Vivo

2'-Fucosyllactose (2'-FL; 100 mg (200 μL); i.g.; daily, for 4 d; C57BL/6 mice with AIEC infection) inhibits AIEC infection and inflammation in vivo^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 mice (8 weeks) with AIEC (uropathogenic E. coli, and adherent-invasive E. coli) infection^[1]
Dosage:

100 mg (200 μL)
Administration:

Oral gavage; daily, for 4 days
Result:

Had colons lengths were closer to normal.
Inhibited the colonisation of the colonic mucosa by O83-positive bacteria.
Decreased CD14 expression, CD14 mRNA levels, IL-6, IL-17 and TNF-α levels in colonic.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 41263-94-9
Appearance Solid
Molecular Weight 488.44
Formula C₁₈H₃₂O₁₅
Color White to off-white
SMILES

O=C[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@H]1[C@@H]([C@H]([C@H]([C@H](O1)CO)O)O)O[C@H]2[C@H]([C@@H]([C@@H]([C@@H](O2)C)O)O)O
Synonyms

2'-FL
Structure Classification
- Saccharides
- Polysaccharides
Initial Source
- Animals
Human milk
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (5)

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Journal Impact Factor

Food Chem

Maillard reaction with milk proteins reduced the prebiotic activity of 2'-fucosyllactose in a neonatal rat model. [Abstract]

2026 Jul 1:516:149410. PMID: 42056776
J Integr Agric

2025 Jun 2.
Int Dairy J

2025 Dec 13;174:106517.
Tissue Cell

Oligosaccharides ameliorate insulin resistance and hepatic metabolism by promoting the leptin/POMC axis to accelerate short stature growth and development. [Abstract]

2025 Dec 13:99:103277. PMID: 41421172
Future Sci OA

The potential of human milk oligosaccharides in ameliorating traumatic brain injury-induced cognitive impairment in mice. [Abstract]

2026 Dec;12(1):2622241. PMID: 41622772

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (102.37 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0473 mL

10.2367 mL

20.4733 mL

5 mM

0.4095 mL

2.0473 mL

4.0947 mL

10 mM

0.2047 mL

1.0237 mL

2.0473 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. He Y, et, al. The human milk oligosaccharide 2'-fucosyllactose modulates CD14 expression in human enterocytes, thereby attenuating LPS-induced inflammation. Gut. 2016 Jan;65(1):33-46. [Content Brief]

[2]. Eiwegger T, et, al. Human milk--derived oligosaccharides and plant-derived oligosaccharides stimulate cytokine production of cord blood T-cells in vitro. Pediatr Res. 2004 Oct;56(4):536-40. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	2.0473 mL	10.2367 mL	20.4733 mL	51.1834 mL
	5 mM	0.4095 mL	2.0473 mL	4.0947 mL	10.2367 mL
	10 mM	0.2047 mL	1.0237 mL	2.0473 mL	5.1183 mL
	15 mM	0.1365 mL	0.6824 mL	1.3649 mL	3.4122 mL
	20 mM	0.1024 mL	0.5118 mL	1.0237 mL	2.5592 mL
	25 mM	0.0819 mL	0.4095 mL	0.8189 mL	2.0473 mL
	30 mM	0.0682 mL	0.3412 mL	0.6824 mL	1.7061 mL
	40 mM	0.0512 mL	0.2559 mL	0.5118 mL	1.2796 mL
	50 mM	0.0409 mL	0.2047 mL	0.4095 mL	1.0237 mL
	60 mM	0.0341 mL	0.1706 mL	0.3412 mL	0.8531 mL
	80 mM	0.0256 mL	0.1280 mL	0.2559 mL	0.6398 mL
	100 mM	0.0205 mL	0.1024 mL	0.2047 mL	0.5118 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

2'-Fucosyllactose

5 Cited Publications

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2'-Fucosyllactose

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Publications Citing Use of MedChemExpress (MCE) 2'-Fucosyllactose

All TNF Receptor Isoforms

All Interleukin Related Isoforms

Biological Activity

Chemical Information

Publications (5)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

2'-Fucosyllactose Related Classifications

HY-N9965 Related Classifications