8RK59
Based on 1 Customer Validation
8RK59, a Bodipy probe, is a potent UCHL1 (ubiquitin C-terminal hydrolase L1) inhibitor, with an IC50 close to 1 μM. 8RK59 could penetrate and label living cells. BodipyFL-alkyne is coupled to the azide of 8RK64 (HY-148254) using copper(I)-mediated click chemistry, resulting in compound 8RK59.
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- Pureté : 99.2%
- CAS No.: 2705841-53-6
- Formule: C33H39BF2N10O2S
- Masse moléculaire:688.60
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Stockage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
IC50 & Target
UCHL1 (ubiquitin C-terminal hydrolase L1)[1]
In Vitro
8RK59 (5 μM, 24 h) binds only to wild-type UCHL1 but not to catalytically inactive UCHL1, indicating that the probe binding site is the active-site cysteine[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Emission (Em)
530
Excitation (Ex)
473
Chemical Information
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CAS No. 2705841-53-6
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Appearance Solid
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Masse moléculaire 688.60
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Formule C33H39BF2N10O2S
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Color Light yellow to orange
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SMILES
O=C(N1CC2=C(N=C(S2)NC([C@@H]3CN(CC3)C#N)=O)CC1)CN(N=N4)C=C4CCCCC(C5=C(C)C=C(C)[N-]5[B+3]([F-])6[F-])=C7[N]6=C(C)C=C7C
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Protocole
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Pureté et documentation
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Fiche technique (268 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
8RK59 Related Classifications
HY-D1726 Related Classifications
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