Bithionol (sulfoxide)

Name: Bithionol (sulfoxide)
Brand: MedChemExpress
SKU: HY-17592A
Rating: 4.5 (1 reviews)

Cat. No.: HY-17592A Purity: 98.65%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Bithionol sulfoxide is an anti-infection agent for parasites. Bithionol sulfoxide has mutagenic activity. Bithionol sulfoxide can be used in the research of parasite infection, such as paragonimiasis, flukes andcestodes infection.

For research use only. We do not sell to patients.

Bithionol (sulfoxide) Chemical Structure

CAS No. : 844-26-8

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 143	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 143	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
1 g		Get quote
5 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Bithionol (sulfoxide):

Bithionol In-stock

1 Publications Citing Use of MCE Bithionol (sulfoxide)

•Patent. US20220324796A1.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Schistosome

In Vitro

Bithionol sulfoxide (0.1-10 mg/mL, 72 h) shows toxicity to Neoparamoeba spp parasites in sea water^[1].
Bithionol sulfoxide (1-1000 μg/plate) exhibits mutagenic activity, in the absence of metabolic activation, in strains TA98 and TA100 (in Salmonella/mammalian microsome test)^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Bithionol sulfoxide (oral administration) shows LD₅₀ values of 1000-5000 mg/kg (mice) and about 5000 mg/kg (rats), and increased serum AST at high doses (5000-100 mg/kg)^[3].
Bithionol sulfoxide (80 and 90 mg/kg, oral administration) is effective against natural rumen fluke infections in dairy cattle^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Dairy cattle^[4]
Dosage:	60, 80 90 mg/kg
Administration:	Oral administration
Result:	Showed approximately 70% effective against rumen flukes infection at dosages of 80 and 90 mg/kg after one week post treatment.

Molecular Weight

372.05

Formula

C₁₂H₆Cl₄O₃S

CAS No.

844-26-8

Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC(Cl)=CC(Cl)=C1O)C2=CC(Cl)=CC(Cl)=C2O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 250 mg/mL (671.95 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6878 mL	13.4391 mL	26.8781 mL
5 mM	0.5376 mL	2.6878 mL	5.3756 mL
10 mM	0.2688 mL	1.3439 mL	2.6878 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (5.59 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.65%

Select Batch:

Data Sheet (278 KB) SDS (251 KB)

COA (251 KB) HNMR (250 KB) RP-HPLC (257 KB) MS (197 KB)

Handling Instructions (2659 KB)

References

[1]. Florent RL, et al. In vitro toxicity of bithionol and bithionol sulphoxide to Neoparamoeba spp., the causative agent of amoebic gill disease (AGD). Dis Aquat Organ. 2010 Sep 17;91(3):257-62. [Content Brief]

[2]. S P Yang, et al. Treatment of paragonimiasis with bithionol and bithionol sulfoxide. Dis Chest. 1967 Aug;52(2):220-32. [Content Brief]

[3]. Lavrič A, et al. Oral toxicity of bithionol sulfoxide in mice and rats. Zbornik Veterinarske Fakultete Univerza Ljubljana 1990 Vol.27 No.1 pp.33-39 ref.22.

[4]. Chompoochan T, et al. The anthelmintic effects of bithionol sulfoxide against sheep experimentally infected with Fasciola gigantica[C]//ACIAR PROCEEDINGS. 1996: 189-194.

[5]. D.Mourot, et al. Mutagenicity of bithionol sulfoxide and its metabolites in the Salmonella/mammalian microsome test. Mutation Research/Genetic Toxicology, 1987, 188(1): 53-55.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6878 mL	13.4391 mL	26.8781 mL	67.1953 mL
	5 mM	0.5376 mL	2.6878 mL	5.3756 mL	13.4391 mL
	10 mM	0.2688 mL	1.3439 mL	2.6878 mL	6.7195 mL
	15 mM	0.1792 mL	0.8959 mL	1.7919 mL	4.4797 mL
	20 mM	0.1344 mL	0.6720 mL	1.3439 mL	3.3598 mL
	25 mM	0.1075 mL	0.5376 mL	1.0751 mL	2.6878 mL
	30 mM	0.0896 mL	0.4480 mL	0.8959 mL	2.2398 mL
	40 mM	0.0672 mL	0.3360 mL	0.6720 mL	1.6799 mL
	50 mM	0.0538 mL	0.2688 mL	0.5376 mL	1.3439 mL
	60 mM	0.0448 mL	0.2240 mL	0.4480 mL	1.1199 mL
	80 mM	0.0336 mL	0.1680 mL	0.3360 mL	0.8399 mL
	100 mM	0.0269 mL	0.1344 mL	0.2688 mL	0.6720 mL