2. Anti-infection
  3. Bacterial Antibiotic
  4. Gramicidin

Gramicidin is an antimicrobial peptide assembling as channels in membranes and increasing their permeability towards cations.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CAS No. : 1405-97-6

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Customer Review

Based on 7 publication(s) in Google Scholar

Gramicidin Related Antibodies

Top Publications Citing Use of Products

    Gramicidin purchased from MedChemExpress. Usage Cited in: PLoS Biol. 2025 Sep 17;23(9):e3002993.  [Abstract]

    Fluorescence intensity change (ΔF/F) time course of RGEPO2 with stimulation by a series of K+ buffer on HEK293FTZZ cells using 10 μg/mL Gramicidin for 15 min.

    Gramicidin purchased from MedChemExpress. Usage Cited in: EMBO J. 2024 Nov;43(22):5469-5493.  [Abstract]

    PS-primed wildtype (WT) or ZNFX1 knockout (KO) THP-1 derived macrophages and Znfx1 KO iBMDM were treated with the indicated inflammation agonists, caspase-1 and IL-1β in the supernatant (sup.) and cell lysate (Lys.) were separated by SDS-PAGE and immunoblotted with the indicated antibodies. Con control, Nig nigericin, Gram Gramicidin (1 μM; 2 h).

    Gramicidin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Jun 21;13(1):3544.  [Abstract]

    Mouse peritoneal macrophages treated with or without 500 ng/ml LPS in the presence or absence of elevated [K+]e (50 mM), Gramicidin (1.25 μM), ML133 (25 μM), PAP1 (Kv1.3 inhibitor, 1 μM), Tram34 (KCa3.1 inhibitor, 1 μM) for 6 h followed by qPCR analysis of mRNA transcription.

    Gramicidin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Jun 21;13(1):3544.  [Abstract]

    Mass isotopomer distribution analysis of metabolites derived from glucose in mouse peritoneal macrophages treated with 500 ng/ml LPS for 6 h in the presence of physiological or elevated [K+]e (50 mM) or Gramicidin (1.25 μM) in medium containing U-[13C]-glucose (25 mM) (n = 5).

    Gramicidin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Jun 21;13(1):3544.  [Abstract]

    Western blots of membrane GLUT1 expression assays in peritoneal macrophages treated with or without 500 ng/ml LPS in the presence or absence of ML133 (25 μM), elevated [K+]e (50 mM) or Gramicidin (1.25 μM) for 2 h (left panel, representative result; right panel, statistics of bands intensity).

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    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Gramicidin is an antimicrobial peptide assembling as channels in membranes and increasing their permeability towards cations.

    IC50 & Target

    Bacterial[1]
    In Vitro

    Gramicidin is an antimicrobial peptide assembling as channels in membranes and increasing their permeability towards cations. There is poor Gramicidin activity against E. coli and S. cerevisae as shown from the survival of microbes (%) over a range of Gramicidin concentrations, in contrast with the action of Gramicidin on S. aureus. The microbicidal activity occurs over a range of low Gramicidin and DODAB concentrations which are not toxic to S. cerevisae[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Gramicidin Related Antibodies
    Clinical Trial
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    Structure Classification
    Initial Source

    membrane pentadecapeptide
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  5% DMSO    40% PEG300    5% Tween-80    50% Saline

      Solubility: 10 mg/mL; Suspended solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  5% DMSO    95% Corn Oil

      Solubility: 10 mg/mL; Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.5%

    SDS (393 KB)

    COA (244 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    Microbes and dispersions are mixed and interacted for one hour before being diluted up to 20,000-fold for plating of 0.1 mL of each in triplicate and incubation (24 hours for 37°C) for CFU counting. Cell survival (%), taken as the mean±standard deviation, is plotted against DODAB and/or Gramicidin concentration. As a control for cell viability in the absence of DODAB or DODAB/Gramicidin dispersions, a standard bacterial suspension is added to 0.264 M D-glucose solution, diluted, and spreaded on the agar plate[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References
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    • If you have published this work, please enter the PubMed ID.
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    Gramicidin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Gramicidin1405-97-6BacterialAntibioticInhibitorinhibitorinhibit

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