Cat. No.: HY-75342

Methyl vanillate

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Methyl vanillate, one of the ingredients in Oryza sativa Linn., is a Wnt/β-catenin pathway activator. A benzoate ester that is the methyl ester of vanillic acid. It has a role as an antioxidant and a plant metabolite.

For research use only. We do not sell to patients.

Purity: 99.88%
CAS No.: 3943-74-6
Formula: C₉H₁₀O₄
Molecular Weight:182.17

Storage:

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Methyl vanillate Chemical Structure

CAS No.: 3943-74-6

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(In Japan)

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Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

Methyl vanillate, one of the ingredients in Oryza sativa Linn., is a Wnt/β-catenin pathway activator^[1]. A benzoate ester that is the methyl ester of vanillic acid. It has a role as an antioxidant and a plant metabolite.

IC₅₀ & Target

Human Endogenous Metabolite

All Wnt Isoforms :

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>100 μM Compound: 1bMe	Anticancer activity against human A549 cells after 24 hrs by MTT assay Anticancer activity against human A549 cells after 24 hrs by MTT assay	[PMID: 20466556]
B16-F10	IC₅₀	>100 μM Compound: 1bMe	Anticancer activity against mouse B16F10 cells after 24 hrs by MTT assay Anticancer activity against mouse B16F10 cells after 24 hrs by MTT assay	[PMID: 20466556]
Hs 683	IC₅₀	>100 μM Compound: 1bMe	Anticancer activity against human Hs683 cells after 24 hrs by MTT assay Anticancer activity against human Hs683 cells after 24 hrs by MTT assay	[PMID: 20466556]
LoVo	IC₅₀	83 μM Compound: 1bMe	Anticancer activity against human LoVo cells after 24 hrs by MTT assay Anticancer activity against human LoVo cells after 24 hrs by MTT assay	[PMID: 20466556]
MCF7	IC₅₀	>100 μM Compound: 1bMe	Anticancer activity against human MCF7 cells after 24 hrs by MTT assay Anticancer activity against human MCF7 cells after 24 hrs by MTT assay	[PMID: 20466556]
OE33	IC₅₀	>100 μM Compound: 1bMe	Anticancer activity against human OE33 cells after 24 hrs by MTT assay Anticancer activity against human OE33 cells after 24 hrs by MTT assay	[PMID: 20466556]
PC-3	IC₅₀	>100 μM Compound: 1bMe	Anticancer activity against human PC3 cells after 24 hrs by MTT assay Anticancer activity against human PC3 cells after 24 hrs by MTT assay	[PMID: 20466556]
RBL-2H3	IC₅₀	640 μM Compound: 1	Anti-allergic activity in RBL2H3 cells assessed as inhibition of anti-DNP-IgE-induced degranulation incubated for 20 mins followed by DNP-labeled human serum albumin addition measured after 1 hr by beta-hexosaminidase release assay Anti-allergic activity in RBL2H3 cells assessed as inhibition of anti-DNP-IgE-induced degranulation incubated for 20 mins followed by DNP-labeled human serum albumin addition measured after 1 hr by beta-hexosaminidase release assay	[PMID: 27324979]
T98G	IC₅₀	>100 μM Compound: 1bMe	Anticancer activity against human T98G cells after 24 hrs by MTT assay Anticancer activity against human T98G cells after 24 hrs by MTT assay	[PMID: 20466556]
U-373MG ATCC	IC₅₀	>100 μM Compound: 1bMe	Anticancer activity against human U373 cells after 24 hrs by MTT assay Anticancer activity against human U373 cells after 24 hrs by MTT assay	[PMID: 20466556]

In Vitro

Methyl vanillate (0-100 μM) increases the expression and nuclear translocation of β-catenin in calvarial osteoblasts. Methyl vanillate dose-dependently elevates ALP activity in calvarial osteoblasts^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 3943-74-6
Appearance Solid
Molecular Weight 182.17
Formula C₉H₁₀O₄
Color Off-white to light brown
SMILES

O=C(OC)C(C=C1)=CC(OC)=C1O
Structure Classification
- Phenols
- Monophenols
Initial Source
- Microorganisms
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Publications (2)

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Journal Impact Factor

Cell Death Dis

The anthelmintic drug niclosamide induces GSK-β-mediated β-catenin degradation to potentiate gemcitabine activity, reduce immune evasion ability and suppress pancreatic cancer progression. [Abstract]

2022 Feb 3;13(2):112. PMID: 35115509
Med Oncol

FAT4 activation inhibits epithelial-mesenchymal transition (EMT) by promoting autophagy in H2228/Cer cells. [Abstract]

2022 Dec 28;40(1):64. PMID: 36576661

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (548.94 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1.67 mg/mL (9.17 mM; Need ultrasonic)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.4894 mL

27.4469 mL

54.8938 mL

5 mM

1.0979 mL

5.4894 mL

10.9788 mL

10 mM

0.5489 mL

2.7447 mL

5.4894 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (13.72 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (13.72 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (13.72 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 2 mg/mL (10.98 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Cha PH, et al. Hovenia dulcis Thunb extract and its ingredient methyl vanillate activate Wnt/β-catenin pathway and increase bone mass in growing or ovariectomized mice. PLoS One. 2014 Jan 22;9(1):e85546. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	5.4894 mL	27.4469 mL	54.8938 mL	137.2345 mL
H₂O / DMSO	5 mM	1.0979 mL	5.4894 mL	10.9788 mL	27.4469 mL
DMSO	10 mM	0.5489 mL	2.7447 mL	5.4894 mL	13.7234 mL
	15 mM	0.3660 mL	1.8298 mL	3.6596 mL	9.1490 mL
	20 mM	0.2745 mL	1.3723 mL	2.7447 mL	6.8617 mL
	25 mM	0.2196 mL	1.0979 mL	2.1958 mL	5.4894 mL
	30 mM	0.1830 mL	0.9149 mL	1.8298 mL	4.5745 mL
	40 mM	0.1372 mL	0.6862 mL	1.3723 mL	3.4309 mL
	50 mM	0.1098 mL	0.5489 mL	1.0979 mL	2.7447 mL
	60 mM	0.0915 mL	0.4574 mL	0.9149 mL	2.2872 mL
	80 mM	0.0686 mL	0.3431 mL	0.6862 mL	1.7154 mL
	100 mM	0.0549 mL	0.2745 mL	0.5489 mL	1.3723 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Methyl vanillate

2 Cited Publications

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Methyl vanillate

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Publications Citing Use of MedChemExpress (MCE) Methyl vanillate

All Wnt Isoforms

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Methyl vanillate Related Classifications

Cat. No.: HY-75342