1. Anti-infection
  2. Bacterial
    Antibiotic
  3. Puromycin dihydrochloride

Puromycin dihydrochloride (Synonyms: CL13900 dihydrochloride)

製品番号: HY-B1743A 純度: 99.87%
取扱説明書

Puromycin dihydrochloride (CL13900 dihydrochloride), an aminonucleoside antibiotic, inhibits protein synthesis.

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Puromycin dihydrochloride 構造式

Puromycin dihydrochloride 構造式

CAS 番号 : 58-58-2

容量 価格(税別) 在庫状況 数量
無料サンプル (0.5-1 mg)   今すぐ申し込む  
10 mM * 1  mL in DMSO USD 55 在庫あり
Estimated Time of Arrival: December 31
10 mg USD 50 在庫あり
Estimated Time of Arrival: December 31
50 mg USD 100 在庫あり
Estimated Time of Arrival: December 31
100 mg   お問い合わせ  
200 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 11 publication(s) in Google Scholar

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製品説明

Puromycin dihydrochloride (CL13900 dihydrochloride), an aminonucleoside antibiotic, inhibits protein synthesis[1].

体外実験

Puromycin blocks protein synthesis after aminoacyl-sRNA formation, and at the same time it leads to the accumulation of small peptides. Both of these effects appear to be due to the splitting of ribosome-bound peptidyl-sRNA,4 which results in release of incomplete peptide chains.[1].
Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Puromycin has two modes of inhibitory action. The first is by acting as an acceptor substrate which attacks peptidyl-tRNA in the P site to form a nascent peptide. The second is by competing with aminoacyl-tRNA for binding to the A' site[2].
When used in minimal amounts, puromycin incorporation in neosynthesized proteins reflects directly the rate of mRNA translation in vitro. Puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling. It allows the direct evaluation of translation activity in single cells by immunofluorescence microscopy and in heterogenous populations of cells by fluorescenceactivated cell sorting[3].

分子量

544.43

分子式

C₂₂H₃₁Cl₂N₇O₅

CAS 番号

58-58-2

SMILES

OC[[email protected]@H]1[[email protected]@H](NC([[email protected]@H](N)CC2=CC=C(OC)C=C2)=O)[[email protected]]([[email protected]](N3C=NC4=C3N=CN=C4N(C)C)O1)O.[H]Cl.[H]Cl

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : ≥ 77.5 mg/mL (142.35 mM)

H2O : 50 mg/mL (91.84 mM; Need ultrasonic and warming)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8368 mL 9.1839 mL 18.3678 mL
5 mM 0.3674 mL 1.8368 mL 3.6736 mL
10 mM 0.1837 mL 0.9184 mL 1.8368 mL
*Please refer to the solubility information to select the appropriate solvent.
体内:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.59 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (3.82 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (3.82 mM); Clear solution

*All of the co-solvents are provided by MCE.
参考文献

純度: 99.87%

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Keywords:

PuromycinCL13900CL 13900CL-13900BacterialAntibioticInhibitorinhibitorinhibit

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Inquiry Information

製品名:
Puromycin dihydrochloride
製品番号:
HY-B1743A
数量:
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