2. Anti-infection
  3. Bacterial Antibiotic Parasite
  4. Puromycin dihydrochloride

Puromycin (dihydrochloride)  (Synonyms: ピューロマイシン二塩酸塩; CL13900 (dihydrochloride))

製品番号: HY-B1743A 純度: 99.93%
COA 取扱説明書 Technical Support

Puromycin dihydrochloride (CL13900 dihydrochloride), an aminonucleoside antibiotic, inhibits protein synthesis.

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CAS 番号 : 58-58-2

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>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 38 在庫あり
Solution
10 mM * 1 mL in DMSO USD 38 在庫あり
Solid
5 mg $32 在庫あり
10 mg $50 在庫あり
25 mg $80 在庫あり
50 mg $150 在庫あり
100 mg $240 在庫あり
500 mg $720 在庫あり
1 g   お問い合わせ  
5 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 387 publication(s) in Google Scholar

Other Forms of Puromycin dihydrochloride:

Puromycin (dihydrochloride) 関連抗体

Top Publications Citing Use of Products

顧客検証

Others
IF
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    Puromycin dihydrochloride purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Nov 6;15(1):9578.  [Abstract]

    Viral titers of supernatants from Huh7.0 FAM134B WT cells, Huh7.0 FAM134B KO cells, Huh7.0 FAM134B KO cells (1 μg/mL, 48 h) stably expressing FAM134BWT or Huh7.0 FAM134B KO cells stably expressing FAM134BR142A, infected with ZIKV at an MOI of 1 for 72 h, quantified by plaque assay.

    Puromycin dihydrochloride purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Jun;21(6):1192-1211.  [Abstract]

    Wild-type or mutated A549 cells subjected to the indicated treatments were stably transfected with a puromycin-inducible GFP-LC3 lentivirus at an MOI of 5. Autophagosomes levels were represented by the presence of GFP-LC3 in cells (A scale bar: 10 μm; B scale bar: 20 μm) (left panel).

    Puromycin dihydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Dec 21;83(24):4494-4508.e6.  [Abstract]

    Global protein synthesis analyzed by puromycin dihydrochloride (4 d) labeling assay upon METTL14 knockdown in HeLa cells. Silencing of EIF3A serves as a positive control.

    Puromycin dihydrochloride purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Dec 21;83(24):4494-4508.e6.  [Abstract]

    Global protein synthesis analyzed by puromycin dihydrochloride (4 d) labeling assay upon IGF2BP1-3 knockdown in HeLa cells.

    Antibiotic アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

    Parasite アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

    • 生物活性

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    製品説明

    Puromycin dihydrochloride (CL13900 dihydrochloride), an aminonucleoside antibiotic, inhibits protein synthesis[1].

    IC50 & Target

    Aminoglycoside

     

    体外実験

    Puromycin blocks protein synthesis after aminoacyl-sRNA formation, and at the same time it leads to the accumulation of small peptides. Both of these effects appear to be due to the splitting of ribosome-bound peptidyl-sRNA,4 which results in release of incomplete peptide chains.[1].
    Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Puromycin has two modes of inhibitory action. The first is by acting as an acceptor substrate which attacks peptidyl-tRNA in the P site to form a nascent peptide. The second is by competing with aminoacyl-tRNA for binding to the A' site[2].
    When used in minimal amounts, puromycin incorporation in neosynthesized proteins reflects directly the rate of mRNA translation in vitro. Puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling. It allows the direct evaluation of translation activity in single cells by immunofluorescence microscopy and in heterogenous populations of cells by fluorescenceactivated cell sorting[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Puromycin (dihydrochloride) 関連抗体
    分子量

    544.43

    分子式

    C22H31Cl2N7O5
    CAS 番号
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    OC[C@@H]1[C@@H](NC([C@@H](N)CC2=CC=C(OC)C=C2)=O)[C@H]([C@H](N3C=NC4=C3N=CN=C4N(C)C)O1)O.[H]Cl.[H]Cl
    Structure Classification
    Initial Source

    Streptomyces alboniger
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    Methanol : 125 mg/mL (229.60 mM; Need ultrasonic)

    H2O : 50 mg/mL (91.84 mM; Need ultrasonic and warming)

    DMSO : 50 mg/mL (91.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8368 mL 9.1839 mL 18.3678 mL
    5 mM 0.3674 mL 1.8368 mL 3.6736 mL
    10 mM 0.1837 mL 0.9184 mL 1.8368 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

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    体積 （開始）

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    濃度 （終了）

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    体積 （終了）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (4.59 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (4.59 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (183.68 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.93%

    COA (255 KB) HNMR (295 KB) RP-HPLC (262 KB) MS (68 KB) Elemental Analysis Report (113 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO / Methanol 1 mM 1.8368 mL 9.1839 mL 18.3678 mL 45.9196 mL
    5 mM 0.3674 mL 1.8368 mL 3.6736 mL 9.1839 mL
    10 mM 0.1837 mL 0.9184 mL 1.8368 mL 4.5920 mL
    15 mM 0.1225 mL 0.6123 mL 1.2245 mL 3.0613 mL
    20 mM 0.0918 mL 0.4592 mL 0.9184 mL 2.2960 mL
    25 mM 0.0735 mL 0.3674 mL 0.7347 mL 1.8368 mL
    30 mM 0.0612 mL 0.3061 mL 0.6123 mL 1.5307 mL
    40 mM 0.0459 mL 0.2296 mL 0.4592 mL 1.1480 mL
    50 mM 0.0367 mL 0.1837 mL 0.3674 mL 0.9184 mL
    60 mM 0.0306 mL 0.1531 mL 0.3061 mL 0.7653 mL
    80 mM 0.0230 mL 0.1148 mL 0.2296 mL 0.5740 mL
    Methanol 100 mM 0.0184 mL 0.0918 mL 0.1837 mL 0.4592 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Puromycin dihydrochloride Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Puromycin58-58-2CL13900CL 13900CL-13900BacterialAntibioticParasiteInhibitorinhibitorinhibit

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    製品名:
    Puromycin dihydrochloride
    製品番号:
    HY-B1743A
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