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  3. Puromycin dihydrochloride

Puromycin dihydrochloride  (Synonyms: CL13900 dihydrochloride)

製品番号: HY-B1743A 純度: 99.89%
COA 取扱説明書

Puromycin dihydrochloride (CL13900 dihydrochloride), an aminonucleoside antibiotic, inhibits protein synthesis.

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Puromycin dihydrochloride 構造式

Puromycin dihydrochloride 構造式

CAS 番号 : 58-58-2

容量 価格(税別) 在庫状況 数量
無料サンプル (0.1 - 0.5 mg)   今すぐ申し込む  
Solution
10 mM * 1 mL in DMSO USD 55 在庫あり
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 55 在庫あり
Estimated Time of Arrival: December 31
Solid
10 mg USD 50 在庫あり
Estimated Time of Arrival: December 31
50 mg USD 150 在庫あり
Estimated Time of Arrival: December 31
100 mg USD 240 在庫あり
Estimated Time of Arrival: December 31
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

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製品説明

Puromycin dihydrochloride (CL13900 dihydrochloride), an aminonucleoside antibiotic, inhibits protein synthesis[1].

IC50 & Target

Aminoglycoside

 

体外実験

Puromycin blocks protein synthesis after aminoacyl-sRNA formation, and at the same time it leads to the accumulation of small peptides. Both of these effects appear to be due to the splitting of ribosome-bound peptidyl-sRNA,4 which results in release of incomplete peptide chains.[1].
Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Puromycin has two modes of inhibitory action. The first is by acting as an acceptor substrate which attacks peptidyl-tRNA in the P site to form a nascent peptide. The second is by competing with aminoacyl-tRNA for binding to the A' site[2].
When used in minimal amounts, puromycin incorporation in neosynthesized proteins reflects directly the rate of mRNA translation in vitro. Puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling. It allows the direct evaluation of translation activity in single cells by immunofluorescence microscopy and in heterogenous populations of cells by fluorescenceactivated cell sorting[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

544.43

分子式

C22H31Cl2N7O5

CAS 番号
SMILES

OC[[email protected]@H]1[[email protected]@H](NC([[email protected]@H](N)CC2=CC=C(OC)C=C2)=O)[[email protected]]([[email protected]](N3C=NC4=C3N=CN=C4N(C)C)O1)O.[H]Cl.[H]Cl

Structure Classification
Source

Streptomyces alboniger

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶剤 & 溶解度
体外: 

H2O : 50 mg/mL (91.84 mM; Need ultrasonic and warming)

DMSO : 50 mg/mL (91.84 mM; Need ultrasonic)

Ethanol : 5 mg/mL (9.18 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8368 mL 9.1839 mL 18.3678 mL
5 mM 0.3674 mL 1.8368 mL 3.6736 mL
10 mM 0.1837 mL 0.9184 mL 1.8368 mL
*Please refer to the solubility information to select the appropriate solvent.
体内:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (183.68 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.59 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (3.82 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (3.82 mM); Clear solution

  • 5.

    Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.5 mg/mL (0.92 mM); Clear solution

  • 6.

    Add each solvent one by one:  10% EtOH    90% corn oil

    Solubility: 0.5 mg/mL (0.92 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are available by MCE.
純度とドキュメンテーション

純度: 99.89%

参考文献
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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製品名:
Puromycin dihydrochloride
製品番号:
HY-B1743A
数量:
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