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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Art. -Nr. Produktname Wirkung Reinheit Chemical Structure
  • HY-D1119
    AF647-NHS ester
    AF647-NHS ester is an analog of Alexa Fluor 647 (AF647). NHS ester can covalently bind to molecules with amino groups (such as proteins, antibodies, etc.). AF647 is a far-red fluorescent dye with an excitation wavelength (λex) of 635 nm (conventional fluorescence detection)/620 nm (instantaneous detection). Storage: Protect from light.
    AF647-NHS ester
  • HY-W751238
    Dimethyl methylene blue zinc chloride double salt
    		99.64%
    Dimethyl methylene blue zinc chloride double salt (DMMB) is a cationic dye. Dimethyl methylene blue zinc chloride double salt competitively binds to the active site of the PANI-Benz-Ag nanocomposite with endotoxin, releasing DMMB and increasing the fluorescence intensity. Dimethyl methylene blue zinc chloride double salt can be used as a fluorescent probe for the detection of bacteria (such as E. Coli) endotoxin. Dimethyl methylene blue zinc chloride double salt can be used for the quantification of sulfated glycosaminoglycans.
    Dimethyl methylene blue zinc chloride double salt
  • HY-D0084
    3,3'-Dihexyloxacarbocyanine iodide
    		99.62%
    3,3'-Dihexyloxacarbocyanine iodide is a carbocyanine dye which can be used to monitor changes in mitochondrial membrane potential.
    3,3'-Dihexyloxacarbocyanine iodide
  • HY-NP0132
    Griffonia Simplicifolia Lectin I Isolectin B4 (Fluorescein)
    Griffonia Simplicifolia Lectin I Isolectin B4 (GSL I Isolectin B4) Fluorescein is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Griffonia Simplicifolia Lectin I Isolectin B4 (GSL I Isolectin B4) Fluorescein is a biological material or organic compound that can be used in life science research.
    Griffonia Simplicifolia Lectin I Isolectin B4 (Fluorescein)
  • HY-D0722
    5(6)-CFDA
    		98.75%
    5(6)-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase. CF containing portion contains an additional negative charge so that it is better retained in cells, compared to fluorescein dyes.
    5(6)-CFDA
  • HY-159529
    DBCO-PEG4-Eosin 5-isothiocyanate
    DBCO-PEG4-Eosin 5-isothiocyanate (DBCO-PEG4-Eosin Y) is a fluorescent probe. DBCO-PEG4-Eosin 5-isothiocyanate contains a clickable functional group, DBCO, and four PEG units. DBCO-PEG4-Eosin 5-isothiocyanate can be used for various imaging detections.
    DBCO-PEG4-Eosin 5-isothiocyanate
  • HY-D0817
    Rhodamine 110
    		99.81%
    Rhodamine 110 is a sensitive and selective substrate for assaying proteinases in solution or inside living cells. The excitation wavelength is 498 nm and the emission wavelength is 521 nm.
    Rhodamine 110
  • HY-D0193
    Ponceau 4R
    		99.84%
    Ponceau 4R is a synthetic colorant that can be used for food coloring. Ponceau 4R is a strawberry red azo dye that can be used in a variety of foods, usually synthesized from aromatic hydrocarbons, and is stable to light, heat, and acids.
    Ponceau 4R
  • HY-D0221
    2,4,6-Tri-2-pyridinyl-1,3,5-triazine
    		99.87%
    2,4,6-Tri-2-pyridinyl-1,3,5-triazine (NSC 112125) is a colorimetric reagent for detecting iron. 2,4,6-Tri-2-pyridinyl-1,3,5-triazine forms a complex with Fe (II) and can be quantified as a measure of iron concentration by colorimetric detection at 594 nm.
    2,4,6-Tri-2-pyridinyl-1,3,5-triazine
  • HY-D2295
    Mito-FerroGreen
    		98%
    Mito-FerroGreen is a mitochondria-specific ferrous ion detection probe with an excitation wavelength of 488nm.
    Mito-FerroGreen
  • HY-D1553
    BODIPY-C12
    		99.90%
    BODIPY-C12 is a boron-dipyrromethene derivative containing a lipophilic tail (excitation 490 nm). BODIPY-C12 is a hydrophobic molecular and sensitivity to the surrounding environment and can be used to quantify viscosity, critical micelle concentration, and critical micelle temperature.
    BODIPY-C12
  • HY-D1781
    NTPAN-MI
    		98.78%
    NTPAN-MI is a fluorescent probe (excitation wavelength: 405 nm). NTPAN-MI is selectively activated after labeling unfolded proteins with exposed thiols, thereby reporting the degree of protein homeostasis.
    NTPAN-MI
  • HY-162543
    18:1 Liss Rhod PE
    		99%
    18:1 Liss Rhod (18:1 Lissamine rhodamine) PE is a fluorescent phospholipid and fluorescent probe.18:1 Liss Rhod PE admixes into phospholipid inks for large-scale monitoring of dip-pen nanolithography-generated lithographic structures via fluorescence microscopy.18:1 Liss Rhod PE undergoes phase separation or self-quenching under certain conditions in thin lipid membrane stacks.
    18:1 Liss Rhod PE
  • HY-P0028
    Phalloidin
    		99.83%
    Phalloidin is a mushroom-derived toxin which can be used to label F-actin of the cytoskeleton with fluorochrome .
    Phalloidin
  • HY-DY1002
    H2DCFDA (solution)
    H2DCFDA (DCFH-DA) (solution) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm) .
    Solvent and concentration: DMSO: 10 mM.
    The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
    H2DCFDA (solution)
  • HY-D0935A
    4-Methylumbelliferyl-β-D-glucuronide hydrate
    		99.92%
    4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm, λem=445 nm). 4-Methylumbelliferyl-β-D-glucuronide hydrate has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli.
    4-Methylumbelliferyl-β-D-glucuronide hydrate
  • HY-D0232
    Brilliant Blue R250
    Brilliant blue R250 (Brilliant Blue R), an anionic dye, is the most popular stain to detect proteins resolved in SDS-PAGE gels.
    Brilliant Blue R250
  • HY-D0056
    5-Carboxyfluorescein diacetate N-succinimidyl ester
    		99.30%
    5-Carboxyfluorescein diacetate N-succinimidyl ester is a cell permeable dye (Ex=492 nm, Em=517 nm). 5-Carboxyfluorescein diacetate N-succinimidyl ester can label cells by covalently binding to intracellular molecules. 5-Carboxyfluorescein diacetate N-succinimidyl ester is used to track lymphocyte migration and proliferation.
    5-Carboxyfluorescein diacetate N-succinimidyl ester
  • HY-NP091
    Wheat Germ Agglutinin
    Wheat Germ Agglutinin (WGA) is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Wheat Germ Agglutinin (WGA) is a biological material or organic compound that can be used in life science research.
    Wheat Germ Agglutinin
  • HY-158082A
    TRITC-dextran, MW 20000
    TRITC-dextran, MW 20000 (Tetramethyl rhodamine isothiocyanate glucan, MW 20000) is a fluorescent dye, with the molecular weight of 20 kD. TRITC-dextran MW 20000 exhibits an excitation wavelength of 555 nm. TRITC-dextran MW 20000 is vessel penetrate, which could label blood plasma to visualize the vasculature. TRITC-dextran MW 20000 is utilized in drug delivery for the stability of TRITC over a wide pH range (i.e. pH 2–11) and resistance to photo-bleaching.
    TRITC-dextran, MW 20000
Art. -Nr. Produktname / Synonyms Application Reactivity