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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-DY1046
    Green CMFDA (solution)
    Green CMFDA (solution) is a cell-permeable fluorescent probe with Em of 514 nm and Ex of 485 nm and can be used as a cell tracer. Green CMFDA can be cleaved by non-specific esterases common in living cells, producing a fluorescent compound, fluorescein, visible using a fluorescent microscope.
    Solvent and concentration: DMSO: 10 mM
    Green CMFDA (solution)
  • HY-D1352A
    Sulfo-Cyanine7 NHS ester TEA
    Sulfo-Cyanine7 NHS ester (TEA) is an amine-reactive succinimide ester. Sulfo-Cyanine7 NHS ester (TEA) reagent allows to prepare sulfo-Cyanine7-labeled biomolecules, such as proteins, with ease. Dye labeled molecules can be subsequently used for various research and agent design related experiments.
    Sulfo-Cyanine7 NHS ester TEA
  • HY-NP087
    Lens Culinaris Agglutinin
    Lens Culinaris Agglutinin (LCA) is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Lens Culinaris Agglutinin (LCA) is a biological material or organic compound that can be used in life science research.
    Lens Culinaris Agglutinin
  • HY-W089353
    8-Phenyl-BODIPY 505/515
    		99.78%
    8-Phenyl-BODIPY 505/515 is a molecular rotor based on the BODIPY scaffold, specifically used as a microviscosity probe. The working principle of 8-Phenyl-BODIPY 505/515 is based on the restriction of molecular motion by viscosity: in a high-viscosity environment, the rotational freedom of its chemical bonds is inhibited, which leads to a significant extension of fluorescence lifetime. This property makes 8-Phenyl-BODIPY 505/515 an effective tool for monitoring changes in the viscosity of intracellular microenvironments via fluorescence lifetime imaging (FLIM) technology.
    8-Phenyl-BODIPY 505/515
  • HY-D2246
    ICG-SH
    ICG-SH?is a negatively charged polymethylcyanine dye.
    ICG-SH
  • HY-D1365
    Sulfo-Cy3(Me)COOH
    Sulfo-Cy3(Me)COOH is a sulfo-Cyanine3 derivative. Cyanine3 is an orange-fluorescent label dye for protein and nucleic acid (Ex=554 nm, Em=568 nm).
    Sulfo-Cy3(Me)COOH
  • HY-D0025
    7-Amino-4-methylcoumarin-3-acetic acid
    		99.85%
    7-Amino-4-methylcoumarin-3-acetic acid (AMCA) serves as the parent structure of coumarin-based blue fluorescent dyes, and its activated form AMCA-NHS can directly conjugate with proteins (Ex/Em ≈ 350/440-460).
    7-Amino-4-methylcoumarin-3-acetic acid
  • HY-120601A
    Alizarin Red S
    Alizarin Red S (ARS) is an anthraquinone derivative dye. When combined with cations such as calcium ions, the functional group of Alizarin Red S can form a coordination bond with the cation through the oxygen atom to show orange-red fluorescence. Alizarin Red S can be used for screening of calcium compounds in synovial fluid and detecting osteoblast differentiation, and can also be used for bone staining in mice. Excitation/emission wavelength: 500/570 nm.
    Alizarin Red S
  • HY-172163C
    FITC-dextran sulfate (MW 500kDa)
    FITC-dextran sulfate, 500kDa is a sodium dextran sulfate labeled with FITC, with an average molecular weight of 500 kDa.
    FITC-dextran sulfate (MW 500kDa)
  • HY-D1336
    FAM amine, 6-isomer
    		98.12%
    FAM amine, 6-isomer is a fluorescein derivative with an amine group and contains an isomer of the fluorophore. Can be used to modify biomolecules through enzymatic transamination. Its fatty amine groups can also react with electrophiles such as activated esters. The amine can also be conjugated to carbonyl compounds (aldehydes and ketones) by reductive amination.
    FAM amine, 6-isomer
  • HY-W248115
    Pyrromethene 597
    		99.67%
    Pyrromethene 597 is a BODIPY laser dye. Pyrromethene 597 displays wide tuning range of lasing wavelengths and high photostability. Pyrromethene 597 can be used as a thermal probe.
    Pyrromethene 597
  • HY-160870
    Firefly luciferase-IN-3
    Firefly luciferase-IN-3 (CID: 17087944) is an inhibitor of ATP-dependent luciferase (Firefly luciferase) and has nM-level inhibitory activity against NanoLuc (pIC50=7.5).
    Firefly luciferase-IN-3
  • HY-D1482
    Sulfo-Cy5-tetrazine
    Sulfo Cy5-tetrazine is a tetrazine-coupled Cy5 (HY-D0821) derivative dye with fluorescence properties similar to CY5 (Ex/Em=633/647 nm). Sulfo-Cy5-tetrazine is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.
    Sulfo-Cy5-tetrazine
  • HY-157036
    INSA
    		98.82%
    INSA is a a pH-sensitive fluorescent probe. INSA can simultaneously light up mitochondria (red emission) and lysosomes (green emission) for their internal pH differences.
    INSA
  • HY-15619
    Hoechst S 769121
    Hoechst S 769121 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution.
    Hoechst S 769121
  • HY-P2500
    (Arg)9, FAM-labeled
    		99.67%
    (Arg)9, FAM-labeled, a cell-penetrating peptide (CPP), is a nona-arginine (ARG) with FAM label. CPPs have emerged as powerful tools for delivering bioactive cargoes into the cytosol of intact cells.
    (Arg)9, FAM-labeled
  • HY-D1719A
    Cypate hydrochloride
    		98.60%
    Cypate is a near-infrared fluorescent dye that belongs to the family of photosensitizers. Cypate has high photostability and optical properties, and is often used in near-infrared optical imaging, as well as optical imaging, tumor marking, and drug delivery. In addition, Cypate is also used as a molecular probe and combined with targeting molecules (such as CBT or small interfering RNA) to achieve efficient detection and imaging of specific cells or tissues.
    Cypate hydrochloride
  • HY-P10094
    β-Ala-Gly-Arg-pNA
    		99.39%
    β-Ala-Gly-Arg-pNA is a chromogenic substrate of thrombin with pNA a strong absorbance at 405 nm.
    β-Ala-Gly-Arg-pNA
  • HY-D1693
    FBBBE
    		99.0%
    FBBBE is used to detect the production of H2O2 by cells. FBBBE can be triggered by intracellular H2O2 and converted to fluorescein, resulting in an increase in intracellular fluorescence (Ex=4480 nm, Em=512 nm).
    FBBBE
  • HY-DY1057
    TMA-DPH (solution)
    TMA-DPH (solution) is a hydrophobic fluorescent membrane probe (Ex=355 nm; Em=430 nm). TMA-DPH is able to anchor on the cell surface and localize to different regions of the phospholipid bilayer. By analyzing the fluorescence polarization values of TMA-DPH in the plasma membrane and membrane substructures, the fluidity of the cell membrane can be determined.
    Solvent and concentration: DMSO: 10 mM
    TMA-DPH (solution)
Cat. No. Product Name / Synonyms Application Reactivity