Cat. No.: HY-32736

Triptonide

(Synonyms: NSC 165677; PG 492)

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

Triptonide (NSC 165677) is a natural product identified in Tripterygium wilfordii Hook F.. Triptonide is a Wnt signaling inhibitor with an IC₅₀ of appropriately 0.3 nM. Triptonide has immunosuppression, anti-inflammatory, anti-fertility, neuroprotective and anti-lymphoma effects.

For research use only. We do not sell to patients.

Purity: 99.75%
CAS No.: 38647-11-9
Formula: C₂₀H₂₂O₆
Molecular Weight:358.39

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Triptonide Chemical Structure

CAS No.: 38647-11-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

IC50: 0.3 nM (Wnt)^[1]

All Wnt Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.0035 μg/mL Compound: 2	Cytotoxicity against human A549 lung tumor cells Cytotoxicity against human A549 lung tumor cells	[PMID: 16455242]
A549	IC₅₀	3.5 ng/mL Compound: 2, triptonide	Cytotoxicity against human A549 cells Cytotoxicity against human A549 cells	[PMID: 18321701]
A549	IC₅₀	0.053 μM Compound: 2	Cytotoxicity against human A549 cells Cytotoxicity against human A549 cells	[PMID: 21470864]
AGS	IC₅₀	<20 nM Compound: 8b	Cytotoxicity against human AGS cells incubated for 72 hrs by Alamar Blue method Cytotoxicity against human AGS cells incubated for 72 hrs by Alamar Blue method	[PMID: 33289552]
ASPC1	IC₅₀	<20 nM Compound: 8b	Antiproliferative activity against human ASPC1 incubated for 72 hrs by alamar blue assay Antiproliferative activity against human ASPC1 incubated for 72 hrs by alamar blue assay	[PMID: 33289552]
BXPC-3	IC₅₀	<20 nM Compound: 8b	Antiproliferative activity against human BXPC-3 incubated for 72 hrs by alamar blue assay Antiproliferative activity against human BXPC-3 incubated for 72 hrs by alamar blue assay	[PMID: 33289552]
CFPAC-1	IC₅₀	<20 nM Compound: 8b	Antiproliferative activity against human CFPAC-1 incubated for 72 hrs by alamar blue assay Antiproliferative activity against human CFPAC-1 incubated for 72 hrs by alamar blue assay	[PMID: 33289552]
DU-145	IC₅₀	0.01 μM Compound: 8b	Antiproliferative activity against human DU-145 cells incubated for 72 hrs by CCK8 assay Antiproliferative activity against human DU-145 cells incubated for 72 hrs by CCK8 assay	[PMID: 33289552]
HGC-27	IC₅₀	<20 nM Compound: 8b	Cytotoxicity against human HGC-27 cells incubated for 72 hrs by Alamar Blue method Cytotoxicity against human HGC-27 cells incubated for 72 hrs by Alamar Blue method	[PMID: 33289552]
HT-29	IC₅₀	<0.00046 μg/mL Compound: 2	Cytotoxicity against human HT29 colon tumor cells Cytotoxicity against human HT29 colon tumor cells	[PMID: 16455242]
HT-29	IC₅₀	<0.46 ng/mL Compound: 2	Cytotoxicity against human HT29 colon tumor cells Cytotoxicity against human HT29 colon tumor cells	[PMID: 16455242]
HT-29	IC₅₀	<0.46 ng/mL Compound: 2, triptonide	Cytotoxicity against human HT29 cells Cytotoxicity against human HT29 cells	[PMID: 18321701]
HT-29	IC₅₀	0.0084 μM Compound: 2	Cytotoxicity against human HT-29 cells Cytotoxicity against human HT-29 cells	[PMID: 21470864]
Jurkat	IC₅₀	4 nM Compound: 8b	Antiproliferative activity against human Jurkat cells incubated for 3 to 6 days by alamar blue assay Antiproliferative activity against human Jurkat cells incubated for 3 to 6 days by alamar blue assay	[PMID: 33289552]
LNCaP	IC₅₀	10 nM Compound: 8b	Antiproliferative activity against human LNCaP cells incubated for 72 hrs by CCK8 assay Antiproliferative activity against human LNCaP cells incubated for 72 hrs by CCK8 assay	[PMID: 33289552]
MCF7	IC₅₀	0.03 μM Compound: Triptonide	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 28759877]
MDA-MB-231	IC₅₀	0.03 μM Compound: Triptonide	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 28759877]
MGC-803	IC₅₀	<20 nM Compound: 8b	Cytotoxicity against human MGC-803 cells incubated for 72 hrs by Alamar Blue method Cytotoxicity against human MGC-803 cells incubated for 72 hrs by Alamar Blue method	[PMID: 33289552]
NCI-N87	IC₅₀	<20 nM Compound: 8b	Cytotoxicity against human NCI-N87 cells incubated for 72 hrs by Alamar Blue method Cytotoxicity against human NCI-N87 cells incubated for 72 hrs by Alamar Blue method	[PMID: 33289552]
PANC-1	IC₅₀	47 nM Compound: Triptonide	Antiproliferative activity against human PANC1 cells assessed as reduction in cell viability after 72 hrs Antiproliferative activity against human PANC1 cells assessed as reduction in cell viability after 72 hrs	[PMID: 31889668]
PANC-1	IC₅₀	<20 nM Compound: 8b	Cytotoxicity against human Panc-1 cells incubated for 72 hrs by alamar blue assay Cytotoxicity against human Panc-1 cells incubated for 72 hrs by alamar blue assay	[PMID: 33289552]
PC-3	IC₅₀	0.6 μM Compound: 2	Antiproliferative activity against human PC3 cells after 72 hrs by SRB assay Antiproliferative activity against human PC3 cells after 72 hrs by SRB assay	[PMID: 20833543]
PC-3	IC₅₀	0.026 μM Compound: 2	Cytotoxicity against human PC3 cells assessed as inhibition of cell proliferation by sulforhodamine B assay Cytotoxicity against human PC3 cells assessed as inhibition of cell proliferation by sulforhodamine B assay	[PMID: 25467158]
PC-3	IC₅₀	0.02 μM Compound: Triptonide	Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 28759877]
PC-3	IC₅₀	25 nM Compound: Triptonide	Antiproliferative activity against human PC3 cells assessed as reduction in cell viability after 72 hrs by CCK8 assay Antiproliferative activity against human PC3 cells assessed as reduction in cell viability after 72 hrs by CCK8 assay	[PMID: 31889668]
PC-3	IC₅₀	0.012 μM Compound: 8b	Antiproliferative activity against human PC-3 cells incubated for 72 hrs by CCK8 assay Antiproliferative activity against human PC-3 cells incubated for 72 hrs by CCK8 assay	[PMID: 33289552]
Raji	IC₅₀	4 nM Compound: 8b	Antiproliferative activity against human Raji cells incubated for 3 to 6 days by alamar blue assay Antiproliferative activity against human Raji cells incubated for 3 to 6 days by alamar blue assay	[PMID: 33289552]
RKO	IC₅₀	15 nM Compound: 8b	Cytotoxicity against human RKO cells incubated for 72 hrs by Cell Titer-Glo luminescence assay Cytotoxicity against human RKO cells incubated for 72 hrs by Cell Titer-Glo luminescence assay	[PMID: 33289552]
SGC-7901	IC₅₀	<20 nM Compound: 8b	Cytotoxicity against human SGC-7901 cells incubated for 72 hrs by Alamar Blue method Cytotoxicity against human SGC-7901 cells incubated for 72 hrs by Alamar Blue method	[PMID: 33289552]
SK-OV-3	IC₅₀	0.008 μM Compound: 2	Antiproliferative activity against human SKOV3 cells after 72 hrs by SRB assay Antiproliferative activity against human SKOV3 cells after 72 hrs by SRB assay	[PMID: 20833543]
SK-OV-3	IC₅₀	0.008 μM Compound: Triptonide	Cytotoxicity against human SKOV3 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human SKOV3 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 28759877]
SW1990	IC₅₀	<20 nM Compound: 8b	Antiproliferative activity against human SW1990 incubated for 72 hrs by alamar blue assay Antiproliferative activity against human SW1990 incubated for 72 hrs by alamar blue assay	[PMID: 33289552]
SW480	IC₅₀	17 nM Compound: 8b	Cytotoxicity against human SW480 cells incubated for 72 hrs by Cell Titer-Glo luminescence assay Cytotoxicity against human SW480 cells incubated for 72 hrs by Cell Titer-Glo luminescence assay	[PMID: 33289552]
U-251	IC₅₀	0.031 μM Compound: 2	Cytotoxicity against human U251 cells assessed as inhibition of cell proliferation by sulforhodamine B assay Cytotoxicity against human U251 cells assessed as inhibition of cell proliferation by sulforhodamine B assay	[PMID: 25467158]

In Vitro

Triptonide blocks Wnt/β-catenin signaling via C-terminal transactivation domain of β-catenin, and promots apoptosis in Wnt-dependent cancer cells^[1].
Triptonide potently inhibits the proliferation of human B-lymphoma Raji and T-lymphoma Jurkat cells with IC₅₀ of 5.7 nM and 4.8 nM, respectively^[2].
Triptonide (2.5-10 nM; 6 days) significantly suppresses B-lymphoma cell colony-forming capability^[2].
Triptonide (20 nM; 3 days) promotes apoptosis through activation of PARP and caspase 3, but reduction of BCL2 protein levels in the lymphoma cells^[2].
Triptonide (5-10 nM; 72 hours) markedly reduces both total and phosphorylated Lyn proteins, and diminishes Lyn downstream ERK and ATK signal pathways^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:

B-lymphoma Raji cells, T-lymphoma Jurkat cells
Concentration:

0-80 nM
Incubation Time:

3 days, 6 days
Result:

Inhibited lymphoma cell tumorigenic capability in a dose-dependent manner.

Apoptosis Analysis^[2]

Cell Line:

Raji cells
Concentration:

5 nM, 10 nM, 20 nM
Incubation Time:

3 days
Result:

Did not significantly induce apoptosis at the effective tumor growth-inhibitory (2.5-10 nM); moderately induced lymphoma cell apoptosis (20 nM).

Western Blot Analysis^[2]

Cell Line:

Raji cells
Concentration:

5 nM, 10 nM, 20 nM
Incubation Time:

3 days
Result:

Did not vigorously activated pro-apoptotic proteins PARP and caspase 3 in lymphoma cells (5-10 nM); significantly activated PARP and caspase 3 (20 nM); significantly reduced anti-apoptotic BCL2 levels.

RT-PCR^[2]

Cell Line:

Raji cells
Concentration:

5 nM, 10 nM
Incubation Time:

72 hours
Result:

Significantly diminished Lyn mRNA levels in the lymphoma cells.

In Vivo

Triptonide (5 mg/kg; i.p.; daily; for 34 days) exerts a strong anti-lymphoma effect in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Eight-week-old female NOD/SCID mice (18-22 g), with 3 x 10⁷ Raji cells xenograft^[2]
Dosage:

5 mg/kg
Administration:

Intraperitoneal injection, daily, for 34 days
Result:

Potently inhibited lymphoma cell growth and tumorigenic capability.

Chemical Information

CAS No. 38647-11-9
Appearance Solid
Molecular Weight 358.39
Formula C₂₀H₂₂O₆
Color White to off-white
SMILES

O=C([C@@]1([C@H]2O1)C(C)C)[C@]34O[C@H]3C[C@@]([H])(C5=C(CC6)C(OC5)=O)[C@@]6(C)[C@]47[C@H]2O7
Synonyms

NSC 165677; PG 492
Structure Classification
- Terpenoids
- Diterpenoids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (4)

All RT-PCRHistological Imaging/Staining

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Journal Impact Factor

Nat Commun

Triptonide is a reversible non-hormonal male contraceptive agent in mice and non-human primates. [Abstract]

2021 Feb 23;12(1):1253. PMID: 33623031
Clin Cancer Res

CDK9 inhibition by dinaciclib is a therapeutic vulnerability in epithelioid hemangioendothelioma. [Abstract]

2024 Sep 13;30(18):4179-4189. PMID: 39052240
Int J Mol Sci

CHIR99021-Treated Osteocytes with Wnt Activation in 3D-Printed Module Form an Osteogenic Microenvironment for Enhanced Osteogenesis and Vasculogenesis. [Abstract]

2023 Mar 22;24(6):6008. PMID: 36983081

Triptonide purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2023 Mar 22;24(6):6008. [Abstract]

qPCR to detect the expression of Wnt target genes in MLO-Y4 cells treated with Triptonide (Trip) (50 nM).

Triptonide purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2023 Mar 22;24(6):6008. [Abstract]

Oil red O staining treated with Triptonide (Trip) (50 nM).
Antiviral Res

Repurposing screen using a robust human rhinovirus infectious clone identifies pyrvinium pamoate with antiviral activity. [Abstract]

2026 Jun:250:106417. PMID: 42025967

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (139.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7903 mL

13.9513 mL

27.9026 mL

5 mM

0.5581 mL

2.7903 mL

5.5805 mL

10 mM

0.2790 mL

1.3951 mL

2.7903 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

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Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (284 KB)
SDS (584 KB)
- English - EN (584 KB)
- Français - FR (584 KB)
- Deutsch - DE (584 KB)
- Norwegian - NO (584 KB)
- Español - ES (584 KB)
- Swedish - SV (584 KB)
- Italian - IT (584 KB)
- Korean - KR (584 KB)
- Portuguese - PT (584 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Jessica Chinison, et al. Triptonide Effectively Inhibits Wnt/β-Catenin Signaling via C-terminal Transactivation Domain of β-catenin. Sci Rep. 2016; 6: 32779. [Content Brief]

[2]. Ping Yang, et al. Triptonide acts as a novel potent anti-lymphoma agent with low toxicity mainly through inhibition of proto-oncogene Lyn transcription and suppression of Lyn signal pathway. Toxicol LettPing Yang. 2017 Aug 15;278:9-17. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.7903 mL	13.9513 mL	27.9026 mL	69.7564 mL
	5 mM	0.5581 mL	2.7903 mL	5.5805 mL	13.9513 mL
	10 mM	0.2790 mL	1.3951 mL	2.7903 mL	6.9756 mL
	15 mM	0.1860 mL	0.9301 mL	1.8602 mL	4.6504 mL
	20 mM	0.1395 mL	0.6976 mL	1.3951 mL	3.4878 mL
	25 mM	0.1116 mL	0.5581 mL	1.1161 mL	2.7903 mL
	30 mM	0.0930 mL	0.4650 mL	0.9301 mL	2.3252 mL
	40 mM	0.0698 mL	0.3488 mL	0.6976 mL	1.7439 mL
	50 mM	0.0558 mL	0.2790 mL	0.5581 mL	1.3951 mL
	60 mM	0.0465 mL	0.2325 mL	0.4650 mL	1.1626 mL
	80 mM	0.0349 mL	0.1744 mL	0.3488 mL	0.8720 mL
	100 mM	0.0279 mL	0.1395 mL	0.2790 mL	0.6976 mL

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Triptonide

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Publications Citing Use of MedChemExpress (MCE) Triptonide

All Wnt Isoforms

Biological Activity

Chemical Information

Publications (4)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Triptonide Related Classifications

HY-32736 Related Classifications