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Art. -Nr.: HY-171859

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FC-14367

FC-14367 is a PROTAC degrader targeting the HIV-1 Nef protein. FC-14367 forms a ternary complex between HIV-1 Nef and CRBN, inducing ubiquitination and proteolytic degradation of Nef. FC-14367 restores the expression of CD4 and MHC-I on the cell surface and inhibits Nef-dependent enhancement of HIV-1 replication. FC-14367 can be used in studies related to HIV-1 infection.
(Pink: Target protein ligand; Blue: Cereblon ligand (HY-138793); Black: linker (HY-W015967)).

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

CAS. Nr.: 3066893-65-7
Formel: C₃₈H₃₅ClFN₇O₆
Molecular Weight:740.18

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (282 KB)

FC-14367 Chemical Structure

CAS. Nr.: 3066893-65-7

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Preis

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Menge

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

FC-14367 is a PROTAC degrader targeting the HIV-1 Nef protein. FC-14367 forms a ternary complex between HIV-1 Nef and CRBN, inducing ubiquitination and proteolytic degradation of Nef. FC-14367 restores the expression of CD4 and MHC-I on the cell surface and inhibits Nef-dependent enhancement of HIV-1 replication. FC-14367 can be used in studies related to HIV-1 infection^[1]. (Pink: Target protein ligand; Blue: Cereblon ligand (HY-138793); Black: linker (HY-W015967)).

IC₅₀ & Target^[1]

HIV-1 Nef

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In Vitro

FC-14367 (90 s association phase, 180 s dissociation phase) binds to diverse M-group HIV-1 Nef variants, SIV Nef_mac239, and the human Cereblon Thalidomide (HY-14658)-binding domain with nanomolar affinity^[1].
FC-14367 (10 μM; 24 h) significantly induces ubiquitylation of HIV-1 Nef in 293T cells at 10 μM^[1].
FC-14367 (3 μM; 48 h) reduces HIV-1 Nef protein levels in CEM/Nef-eGFP T cells to ~65% of control levels after 48 h of incubation^[1].
FC-14367 (1 μM; 4 days) suppresses wild-type HIV-1 NL4-3 replication in activated donor PBMCs at 1 μM to levels equivalent to the Nef-defective mutant, while maintaining near-100% cell viability relative to DMSO controls^[1].
FC-14367 (3 μM; 24 h) induces degradation of HIV-1 Nef-eGFP in CEM/Nef-eGFP T cells, restoring cell-surface CD4 and MHC-I expression to ~75% and ~60% of control levels, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

Doxycycline-inducible CEM/Nef-eGFP T cells
Concentration:

3 μM
Incubation Time:

48 h
Result:

Reduced Nef protein levels to ~65% of DMSO control levels, as measured by Nef-to-Actin band intensity ratios.

Chemical Information

CAS. Nr. 3066893-65-7
Molecular Weight 740.18
Formel C₃₈H₃₅ClFN₇O₆
SMILES

O=C1NC(C(CC1)N2C(C3=C(C2)C(OCC(N4CCC(CC4)C5=NN(C6=NC7=C(N6)C=CC(Cl)=C7)C(O)=C5CCC8=CC=C(C=C8)F)=O)=CC=C3)=O)=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Emert-Sedlak LA, et al. PROTAC-mediated degradation of HIV-1 Nef efficiently restores cell-surface CD4 and MHC-I expression and blocks HIV-1 replication. Cell chemical biology. 2024 Apr 18;31(4):658-668.e14. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise

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FC-14367

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