MSA-2-Pt
MSA-2-Pt, platinum salt-modified MSA-2 (HY-136927), is a STING agonist. MSA-2-Pt inducing cell
death by platinum and activating the STING pathway by MSA-2. MSA-2-Pt direct activates STING pathway, induces phosphorylation of TBK1, IRF3, and NF-κB p65. MSA-2-Pt enhances tumor infiltration of CD4+ and CD8+ T cells, and induces tumor cell death and apoptosis in mouse colon carcinoma and melanoma models.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- CAS. Nr.: 3028778-96-0
- Formel: C28H32N2O10PtS2
- Molecular Weight:815.78
-
Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biologische Aktivität
-
Chemical Information
- Reinheit & Dokumentation
- Verweise
-
Help & FAQs
Biologische Aktivität
death by platinum and activating the STING pathway by MSA-2. MSA-2-Pt direct activates STING pathway, induces phosphorylation of TBK1, IRF3, and NF-κB p65. MSA-2-Pt enhances tumor infiltration of CD4+ and CD8+ T cells, and induces tumor cell death and apoptosis in mouse colon carcinoma and melanoma models[1].
|
TBK1 |
IRF3 |
MSA-2-Pt (25-300 μM; 12-24 h) induces dose-dependent cell death in mouse colon adenocarcinoma MC38 cells, with significant cytotoxicity observed at concentrations ≥75 μM after 24 h[1].
MSA-2-Pt (10-50 μM; 24 h) activates IFN-β secretion in mouse macrophage RAW264.7 cells[1].
MSA-2-Pt (10-50 μM; 3 h) activates the STING signaling pathway in mouse macrophage RAW264.7 cells, inducing phosphorylation of STING, TBK1, IRF3, and NF-κB p65 at concentrations of 10, 25, and 50 μM after 3 h[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:mouse colon adenocarcinoma MC38 cells
-
Concentration:25 μM, 50 μM, 75 μM, 100 μM, 200 μM, 300 μM
-
Incubation Time:12; 24 h;
-
Result:Induced significant MC38 cell death starting at 75 μM, with a dose-dependent reduction in cell viability.
Reduced cell viability to ~20% or lower at 100 μM and above.
Confirmed significant pyroptotic cell death in MC38 cells treated with 75 μM for 12 h and 24 h via microscopic imaging.
-
Cell Line:mouse macrophage RAW264.7 cells
-
Concentration:10 μM, 25 μM, 50 μM
-
Incubation Time:3 h
-
Result:Increased levels of phosphorylated P65 (p-P65) compared to control.
Induced phosphorylation of STING, TBK1, and IRF3 in a concentration-dependent manner.
MSA-2-Pt (150 μg; i.t.; three doses on day 9, 11, 15) exhibits significant antitumor efficacy in poorly immunogenic B16F10 melanoma-bearing mice, with 22% of treated mice achieving tumor-free status and significantly extended survival[1].
MSA-2-Pt (150 μg; i.t.; single dose) activates the STING pathway (via elevated serum IFN-β), enhances tumor infiltration of CD4+ and CD8+ T cells, and induces tumor cell death and apoptosis in MC38 colon carcinoma-bearing mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Colon carcinoma C57BL/6 mice (female, 6 weeks old, subcutaneous injection of 2×105 MC38 cells)[1]
-
Dosage:150 μg
-
Administration:intratumorly injection; three doses on day 13, 15, 19
-
Result:Significantly reduced tumor growth relative to the control group.
Achieved tumor-free status in 7 out of 9 treated mice.
Significantly increased survival compared to the control group.
-
Animal Model:Melanoma C57BL/6 mice (female, 6 weeks old, subcutaneous injection of 2×105 B16F10 cells)[1]
-
Dosage:150 μg
-
Administration:intratumorly injection; three doses
-
Result:Significantly reduced tumor growth relative to the control group.
Achieved tumor-free status in 2 out of 9 treated mice.
Significantly increased survival compared to the control group.
-
Animal Model:Colon carcinoma C57BL/6 mice (female, 6 weeks old, subcutaneous injection of 2×105 MC38 cells)[1]
-
Dosage:150 μg
-
Administration:intratumorly injection; single dose
-
Result:Increased serum IFN-β levels significantly relative to the control group.
Increased the ratio of CD4+ T cells of total cells and CD8+ T cells of total cells in tumor tissue relative to the control group.
Induced significant tumor cell death observed via H&E staining.
Induced a significantly higher proportion of tumor cell apoptosis observed via TUNEL staining.
Chemical Information
-
CAS. Nr. 3028778-96-0
-
Molecular Weight 815.78
-
Formel C28H32N2O10PtS2
-
SMILES
O=C(CCC(C1=CC2=C(C=C(C(OC)=C2)OC)S1)=O)[O-][Pt+2]([NH3])([NH3])[O-]C(CCC(C3=CC4=C(C=C(C(OC)=C4)OC)S3)=O)=O
-
Versand
Room temperature in continental US; may vary elsewhere.
-
Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
-
Data Sheet (275 KB)
-
SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
MSA-2-Pt Related Classifications
HY-162133 Related Classifications
Powered by Bioz