Cat. No.: HY-12031A

U0126

Name: U0126
Brand: MedChemExpress
SKU: HY-12031A
Price: 120.00 USD
Availability: InStock
Rating: 5 (231 reviews)

231 Cited Publications

Customer Review

Based on 231 publication(s) in Google Scholar

U0126 is a potent, non-ATP competitive and selective MEK1 and MEK2 inhibitor, with IC₅₀s of 72 nM and 58 nM, respectively. U0126 is an autophagy and mitophagy inhibitor.

For research use only. We do not sell to patients.

Purity : 98.57%
CAS No.: 109511-58-2
Formula: C₁₈H₁₆N₆S₂
Molecular Weight:380.49

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

U0126 Chemical Structure

CAS No.: 109511-58-2

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (231)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
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Biological Activity

Description

U0126 is a potent, non-ATP competitive and selective MEK1 and MEK2 inhibitor, with IC₅₀s of 72 nM and 58 nM, respectively. U0126 is an autophagy and mitophagy inhibitor^[1]^[2]^[3]^[4].

IC₅₀ & Target

^[1]

MEK2

60 nM (IC₅₀)

MEK1

70 nM (IC₅₀)

All MEK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	1.1 μM Compound: U0126	Antiproliferative activity against human A375 cells assessed as cell viability incubated for 72 hrs by cell titer-glo luminescent cell viability assay (Rvb = > 100 uM) Antiproliferative activity against human A375 cells assessed as cell viability incubated for 72 hrs by cell titer-glo luminescent cell viability assay (Rvb = > 100 uM)	[PMID: 31252285]
A549	IC₅₀	32.2 μM Compound: U0126	Antiproliferative activity against human A549 cells assessed as cell viability incubated for 72 hrs by cell titer-glo luminescent cell viability assay (Rvb = > 100 uM) Antiproliferative activity against human A549 cells assessed as cell viability incubated for 72 hrs by cell titer-glo luminescent cell viability assay (Rvb = > 100 uM)	[PMID: 31252285]
COS-7	IC₅₀	1 μM Compound: U-0126	Inhibitory concentration against AP-1 transcription in COS-7 cells Inhibitory concentration against AP-1 transcription in COS-7 cells	[PMID: 15006386]
DLD-1	IC₅₀	1.14 μM Compound: 40; U0126	Anticancer activity against human DLD-1 cells assessed as reduction in cell viability incubated for 24 hrs by Cell Titer-Glo assay Anticancer activity against human DLD-1 cells assessed as reduction in cell viability incubated for 24 hrs by Cell Titer-Glo assay	[PMID: 33445154]
HCT-116	GI₅₀	19.4 μM Compound: U-0126	Ability of compound to inhibit anchorage independent colony formation (soft agar growth assay) in HCT116 cells Ability of compound to inhibit anchorage independent colony formation (soft agar growth assay) in HCT116 cells	[PMID: 15225706]
HCT-116	IC₅₀	0.94 μM Compound: 40; U0126	Anticancer activity against human HCT-116 cells assessed as reduction in cell viability incubated for 24 hrs by Cell Titer-Glo assay Anticancer activity against human HCT-116 cells assessed as reduction in cell viability incubated for 24 hrs by Cell Titer-Glo assay	[PMID: 33445154]
HeLa	IC₅₀	0.29 μM Compound: U-0126	Inhibition of EGF-stimulated Elk1-luciferase reporter assay in HeLa cells Inhibition of EGF-stimulated Elk1-luciferase reporter assay in HeLa cells	[PMID: 15225706]
HL-60	IC₅₀	0.3 μM Compound: U0126	Antiproliferative activity against human HL60 cells assessed as cell viability incubated for 72 hrs by cell titer-glo luminescent cell viability assay (Rvb = > 100 uM) Antiproliferative activity against human HL60 cells assessed as cell viability incubated for 72 hrs by cell titer-glo luminescent cell viability assay (Rvb = > 100 uM)	[PMID: 31252285]
MDCK	CC₅₀	>50 μM Compound: 79; U0126	Cytotoxicity against MDCK cells Cytotoxicity against MDCK cells	[PMID: 33539089]
Sf21	IC₅₀	0.21 μM Compound: U0126	Inhibition of recombinant N-terminal GST-tagged human BRAF (433-726 residues) expressed in baculovirus infected sf21 insect cells/recombinant human full length N-terminal GST-tagged MEK1 expressed in Escherichia coli cascading pathway assessed as reductio Inhibition of recombinant N-terminal GST-tagged human BRAF (433-726 residues) expressed in baculovirus infected sf21 insect cells/recombinant human full length N-terminal GST-tagged MEK1 expressed in Escherichia coli cascading pathway assessed as reductio	[PMID: 31252285]
Sf9	IC₅₀	50 μM Compound: U-0126	Inhibition of His-tagged human PRAK expressed in Sf9 cells Inhibition of His-tagged human PRAK expressed in Sf9 cells	[PMID: 10998351]
Sf9	IC₅₀	60 μM Compound: U-0126	Inhibition of human PKBalpha expressed in SF9 cells Inhibition of human PKBalpha expressed in SF9 cells	[PMID: 10998351]

In Vitro

Treatment with U0126 efficiently reduces progeny virus titers of all tested strains in A549 cells. While nM concentrations of U0126 are efficient to reduce H1N1v and H5N1 (MB1), μM concentrations of U0126 are required to reduce the virus titer of H5N1 (GSB) and H7N7. The EC₅₀ values for U0126-EtOH against H1N1v are 1.2±0.4 μM in A549 cells and 74.7±1.0 μM in MDCKII cells^[2].
Rat hepatocarcinoma cells (FAO) stimulated by fetal calf serum (FCS) exhibits a significant proportion in S phase (32.62%) whereas U0126 strongly decreases the proportion of cells in S phase (9.92%) and increases the proportion of cells in G₀-G₁ phase and to a lesser extent in G₂/M^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

A549 and MDCK II cells.
Concentration:

0.001-1000 μM.
Incubation Time:

48 h.
Result:

The EC₅₀ values for U0126 against H1N1v were 1.2 ± 0.4 μM in A549 cells and 74.7 ± 1.0 μM in MDCKII cells

In Vivo

Mice are treated daily with U0126-EtOH (U0126; i.p., 10.5 mg/kg). In control experiment, tumor sizes are constant or slightly increase all over the kinetic. At the opposite, in all U0126-EtOH experiments, engraftment and early tumor growth are significantly decreased. Furthermore, a 60-70% reduction in the volume of tumors treated with U0126-EtOH is obtained 9 days after injection and thereafter^[3].
Rats are subjected to 120 minutes transient middle cerebral artery occlusion (tMCAO) and thereafter treated with the U0126-EtOH (U0126; i.p., 30 mg/kg) at 0 and 24 hours of reperfusion. After treatment with U0126-EtOH, the vasoconstriction to S6c is markedly reduced^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Athymic female nude mice (SWISS, nu/nu)^[3].
Dosage:

10.5 mg/kg.
Administration:

Intraperitoneal injection daily.
Result:

Inhibited tumor growth.

Animal Model:

Twelve-week-old female Wistar rats (250 to 265 g) ^[4].
Dosage:

30 mg/kg.
Administration:

Intraperitoneally.
Result:

The vasoconstriction to S6c is markedly reduced.

Chemical Information

CAS No. 109511-58-2
Appearance Solid
Molecular Weight 380.49
Formula C₁₈H₁₆N₆S₂
Color Off-white to light yellow
SMILES

NC1=CC=CC=C1S/C(N)=C(C#N)/C(C#N)=C(N)/SC2=CC=CC=C2N
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (231)

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Journal Impact Factor

Nature

A mechanism to initiate emergency type 2 myelopoiesis. [Abstract]

2026 May;653(8113):212-220. PMID: 41813898
Cell Metab

Adipokine IL-11/IL-11Ra constrains sphingolipid metabolism to limit the thermogenic capacity of beige adipocytes. [Abstract]

2026 May 14:S1550-4131(26)00151-8. PMID: 42140185
Immunity

The chemokine CCL1 triggers an AMFR-SPRY1 pathway that promotes differentiation of lung fibroblasts into myofibroblasts and drives pulmonary fibrosis. [Abstract]

2021 Sep 14;54(9):2042-2056.e8. PMID: 34407391
Cancer Commun (Lond)

Yap methylation-induced FGL1 expression suppresses anti-tumor immunity and promotes tumor progression in KRAS-driven lung adenocarcinoma. [Abstract]

2024 Nov;44(11):1350-1373. PMID: 39340215

U0126 purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2024 Nov;44(11):1350-1373. [Abstract]

Ubiquitination assays in A549 cells showed that U0126 (2 μmol/L, 24 h) augments SET1A ubiquitination.

U0126 purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2024 Nov;44(11):1350-1373. [Abstract]

U0126 (1-20 μmol/L, 24 h) significantly reduced FGL1 levels in KRAS-mutant lung cancer cells H358.
Nat Methods

Reconstruction of dynamic mammary mini gland in vitro for normal physiology and oncogenesis. [Abstract]

2023 Dec;20(12):2021-2033. PMID: 37919421
Nat Immunol

Type A cholesterol-dependent cytolysins translocate to the trans-Golgi network for NLRP3 inflammasome activation. [Abstract]

2025 Oct;26(10):1673-1685. PMID: 40913097

U0126 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1673-1685. [Abstract]

U0126 (20 μM, 30 min) and Mirdametinib enhanced LDH release in rPFO-treated HeLa cells.

U0126 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1673-1685. [Abstract]

U0126 (20 μM, 30 min) and Mirdametinib enhanced rPFO-induced TGN remodeling and NLRP3 recruitment in HeLa NLRP3-GFP cells.

U0126 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1673-1685. [Abstract]

U0126 (20 μM, 30 min) and Mirdametinib enhanced rPFO-induced TGN remodeling and NLRP3 recruitment in HeLa NLRP3-GFP cells.
Clin Mol Hepatol

Taurocholic Acid Promotes Hepatic Stellate Cell Activation via S1PR2/p38 MAPK/YAP Signaling under Cholestatic Conditions. [Abstract]

2023 Apr;29(2):465-481. PMID: 36800698
Nat Commun

INSIG1/2 succination mediated by the moonlighting function of ADSL promotes lipogenesis and liver tumorigenesis. [Abstract]

2026 Mar 15;17(1):4002. PMID: 41833955
Nat Commun

The CXCL8/MAPK/hnRNP-K axis enables susceptibility to infection by EV-D68, rhinovirus, and influenza virus in vitro. [Abstract]

2025 Feb 17;16(1):1715. PMID: 39962077
Nat Commun

Deficiency of the HGF/Met pathway leads to thyroid dysgenesis by impeding late thyroid expansion. [Abstract]

2024 Apr 11;15(1):3165. PMID: 38605010
Exp Hematol Oncol

Combined inhibition of MET and VEGF enhances therapeutic efficacy of EGFR TKIs in EGFR-mutant non-small cell lung cancer with concomitant aberrant MET activation. [Abstract]

2024 Oct 1;13(1):97. PMID: 39354638
Exp Mol Med

MRTF-A-NF-κB/p65 axis-mediated PDL1 transcription and expression contributes to immune evasion of non-small-cell lung cancer via TGF-β. [Abstract]

2021 Sep;53(9):1366-1378. PMID: 34548615
J Adv Res

Ursolic acid reduces Aβ-driven aggression via Gab1-mediated autophagy and dorsal hippocampal circuit modulation in Alzheimer's disease. [Abstract]

2026 Jan 2:S2090-1232(25)01037-9. PMID: 41485573
J Adv Res

The purinergic receptor P2rx7 mediated ATP sensing is required to prevent bone aging by directing mitochondrial fitness of MSCs. [Abstract]

2025 Jun 13:S2090-1232(25)00435-7. PMID: 40518112
Neuron

C5aR1+ microglia exacerbate neuroinflammation and cerebral edema in acute brain injury. [Abstract]

2025 Nov 18:S0896-6273(25)00803-7. PMID: 41260217
J Mater Sci Technol

2024 Jan 24.
Redox Biol

PP4 modulates macrophage-neutrophil crosstalk to restrict CCL5 -driven NETosis in sepsis. [Abstract]

2026 Apr:91:104093. PMID: 41723906
Redox Biol

Reactivation of MAPK-SOX2 pathway confers ferroptosis sensitivity in KRASG12C inhibitor resistant tumors. [Abstract]

2024 Dec:78:103419. PMID: 39527862
Mol Cell

OXCT1 succinylation and activation by SUCLA2 promotes ketolysis and liver tumor growth. [Abstract]

2025 Feb 20;85(4):843-856.e6. PMID: 39862868
Theranostics

ILT4 inhibition prevents TAM- and dysfunctional T cell-mediated immunosuppression and enhances the efficacy of anti-PD-L1 therapy in NSCLC with EGFR activation. [Abstract]

2021 Jan 19;11(7):3392-3416. PMID: 33537094
Genes Dis

2025 Nov 6.
Biomark Res

PAQR5 drives the malignant progression and shapes the immunosuppressive microenvironment of hepatocellular carcinoma by activating the NF-κB signaling. [Abstract]

2025 May 7;13(1):70. PMID: 40336138
Genes Dis

Sulfuretin exerts diversified functions in the processing of amyloid precursor protein. [Abstract]

2020 Nov 21;8(6):867-881. PMID: 34522714
Interdiscip Med

2026 May 18.
J Exp Clin Cancer Res

Overexpressed integrin alpha 2 inhibits the activation of the transforming growth factor β pathway in pancreatic cancer via the TFCP2-SMAD2 axis. [Abstract]

2022 Feb 22;41(1):73. PMID: 35193647
J Exp Clin Cancer Res

Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8+ T cell exclusion. [Abstract]

2021 Jan 4;40(1):4. PMID: 33390170
Adv Sci (Weinh)

Hepatocyte-Derived FGF1 Alleviates Isoniazid and Rifampicin-Induced Liver Injury by Regulating HNF4α-Mediated Bile Acids Synthesis. [Abstract]

2025 Feb;12(7):e2408688. PMID: 39731358
Adv Sci (Weinh)

Osteoblast-Derived ECM1 Promotes Anti-Androgen Resistance in Bone Metastatic Prostate Cancer. [Abstract]

2025 Jan;12(2):e2407662. PMID: 39563492
Adv Sci (Weinh)

TRIM24 Cooperates with Ras Mutation to Drive Glioma Progression through snoRNA Recruitment of PHAX and DNA-PKcs. [Abstract]

2024 Aug;11(29):e2400023. PMID: 38828688
Adv Sci (Weinh)

Opsin-Free Activation of Bmp Receptors by a Femtosecond Laser. [Abstract]

2024 Mar;11(10):e2308072. PMID: 38239021
Cell Rep Med

Targeting the noncanonical function of metabolic enzyme PHGDH in driving PD-L1 expression and cancer immune evasion. [Abstract]

2026 Mar 26:102704. PMID: 41905348
Cell Rep Med

Zeaxanthin augments CD8+ effector T cell function and immunotherapy efficacy. [Abstract]

2025 Sep 16;6(9):102324. PMID: 40897177
Cell Rep Med

MFGE8 induces anti-PD-1 therapy resistance by promoting extracellular vesicle sorting of PD-L1. [Abstract]

2025 Feb 18;6(2):101922. PMID: 39842432
Brain

NINJ1 hypomethylation enhances endothelial dysfunction in brain arteriovenous malformations. [Abstract]

2026 May 26:awag189. PMID: 42189175
Cardiovasc Res

Endothelial CCRL2 induced by disturbed flow promotes atherosclerosis via chemerin-dependent β2 integrin activation in monocytes. [Abstract]

2023 Aug 7;119(9):1811-1824. PMID: 37279540
Cell Death Dis

STUB1-induced polyubiquitination of SIK3 in alveolar type 2 epithelial cells alleviates severity and outcomes of acute lung injury. [Abstract]

2026 May 4;17(1):589. PMID: 42082453
Cell Death Dis

Lactate transmission from hypoxic tumor cells promotes macrophage senescence and M2 polarization via the DNMT1-NHE7 axis to accelerate endometrial cancer progression. [Abstract]

2026 Jan 30;17(1):185. PMID: 41617670
Pharmacol Res

Losartan attenuates sepsis-induced cardiomyopathy by regulating macrophage polarization via TLR4-mediated NF-κB and MAPK signaling. [Abstract]

2022 Sep 28;185:106473. PMID: 36182039
Cell Death Dis

Lenvatinib for effectively treating antiangiogenic drug-resistant nasopharyngeal carcinoma. [Abstract]

2022 Aug 19;13(8):724. PMID: 35985991
Cell Death Dis

Effects of short-chain fatty acids in inhibiting HDAC and activating p38 MAPK are critical for promoting B10 cell generation and function. [Abstract]

2021 Jun 7;12(6):582. PMID: 34099635
Cell Death Dis

SLC7A2 deficiency promotes hepatocellular carcinoma progression by enhancing recruitment of myeloid-derived suppressors cells. [Abstract]

2021 Jun 2;12(6):570. PMID: 34108444
Cell Death Dis

LncRNA CD27-AS1 promotes acute myeloid leukemia progression through the miR-224-5p/PBX3 signaling circuit. [Abstract]

2021 May 18;12(6):510. PMID: 34006845
Cancer Lett

CD24 promotes metastasis and chemoresistance by directly targeting Arf6-ERK pathway in esophageal squamous cell carcinoma. [Abstract]

2024 Jul 10:594:216994. PMID: 38801885
J Immunother Cancer

Spatial and single-cell transcriptomics reveal cellular heterogeneity and a novel cancer-promoting Treg cell subset in human clear-cell renal cell carcinoma. [Abstract]

2025 Jan 4;13(1):e010183. PMID: 39755578
J Immunother Cancer

Targeting CD93 on monocytes revitalizes antitumor immunity by enhancing the function and infiltration of CD8+ T cells. [Abstract]

2024 Oct 23;12(10):e010148. PMID: 39448202
Cell Commun Signal

Targeting the ERK1/2 and p38 MAPK pathways attenuates Golgi tethering factor golgin-97 depletion-induced cancer progression in breast cancer. [Abstract]

2025 Jan 13;23(1):22. PMID: 39800687
eGastroenterology

Gut cannabinoid receptor 1 regulates alcohol binge-induced intestinal permeability. [Abstract]

2025 Feb 12;3(1):e100173. PMID: 40124989
Cell Death Discov

Non-enzymatic function of QSOX2 directly regulates the JUNB-ITGB4 axis and enhanced resistance to osimertinib in EGFR-mutation lung adenocarcinoma. [Abstract]

2026 Apr 1;12(1):215. PMID: 41922310
Cell Death Discov

Nr1d1 inhibition mitigates intermittent hypoxia-induced pulmonary hypertension via Dusp1-mediated Erk1/2 deactivation and mitochondrial fission attenuation. [Abstract]

2024 Oct 29;10(1):459. PMID: 39472573
Cell Death Discov

Ribosomal protein L22-like1 (RPL22L1) mediates sorafenib sensitivity via ERK in hepatocellular carcinoma. [Abstract]

2022 Aug 17;8(1):365. PMID: 35973992
Acta Biomater

Laminin alpha 4 promotes bone regeneration by facilitating cell adhesion and vascularization. [Abstract]

2021 May:126:183-198. PMID: 33711525
Cell Death Discov

TGR5-HNF4α axis contributes to bile acid-induced gastric intestinal metaplasia markers expression. [Abstract]

2020 Jul 6:6:56. PMID: 32655894
Nat Struct Mol Biol

ERK-USP9X-coupled regulation of thymidine kinase 1 promotes both its enzyme activity-dependent and its enzyme activity-independent functions for tumor growth. [Abstract]

2025 May;32(5):853-863. PMID: 39824978
J Orthop Translat

Inhibiting the REV-ERBα expression protects against mechanical overloading-induced cartilage clock disruption and osteoarthritis progression. [Abstract]

2025 Jun 18:53:112-125. PMID: 40606845
J Orthop Translat

SHP2-mediated ROS activation induces chondrocyte paraptosis in osteoarthritis and is attenuated by low-intensity pulsed ultrasound. [Abstract]

2025 Apr 25:52:233-248. PMID: 40337549
J Transl Med

LIPH contributes to glycolytic phenotype in pancreatic ductal adenocarcinoma by activating LPA/LPAR axis and maintaining ALDOA stability. [Abstract]

2023 Nov 21;21(1):838. PMID: 37990271
Proc Natl Acad Sci U S A

2026 Jul 7;123(27):e2523789123. PMID: 42384696
Chin Med J (Engl)

Arsenic trioxide preconditioning attenuates hepatic ischemia- reperfusion injury in mice: Role of ERK/AKT and autophagy. [Abstract]

2025 Jan 17. PMID: 39820060
Oncogene

NRG1 regulates Fra-1 transcription and metastasis of triple-negative breast cancer cells via the c-Myc ubiquitination as manipulated by ERK1/2-mediated Fbxw7 phosphorylation. [Abstract]

2022 Feb;41(6):907-919. PMID: 34992218
Oncogene

LIMK1 nuclear translocation promotes hepatocellular carcinoma progression by increasing p-ERK nuclear shuttling and by activating c-Myc signalling upon EGF stimulation. [Abstract]

2021 Apr;40(14):2581-2595. PMID: 33686242
New Phytol

The Snf5-Hsf1 transcription module synergistically regulates stress responses and pathogenicity by maintaining ROS homeostasis in Sclerotinia sclerotiorum. [Abstract]

2024 Feb;241(4):1794-1812. PMID: 38135652
Neural Regen Res

Eph receptor A4 regulates motor neuron ferroptosis in spinal cord ischemia/reperfusion injury in rats. [Abstract]

2023 Oct;18(10):2219-2228. PMID: 37056141
EMBO J

Ephrin A1 functions as a ligand of EGFR to promote EMT and metastasis in gastric cancer. [Abstract]

2025 Mar;44(5):1464-1487. PMID: 39838173
Cell Mol Gastroenterol Hepatol

Sorafenib Promotes Treg Cell Differentiation To Compromise Its Efficacy via VEGFR/AKT/Foxo1 Signaling in Hepatocellular Carcinoma. [Abstract]

2025;19(5):101454. PMID: 39743020
Antioxidants (Basel)

Lemon Peel Water Extract: A Novel Material for Retinal Health, Protecting Retinal Pigment Epithelial Cells against Dynamin-Related Protein 1-Mediated Mitochondrial Fission by Blocking ROS-Stimulated Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase Pathway. [Abstract]

2024 Apr 27;13(5):538. PMID: 38790643
Antioxidants (Basel)

Characterization of the Inducible and Slow-Releasing Hydrogen Sulfide and Persulfide Donor P*: Insights into Hydrogen Sulfide Signaling. [Abstract]

2021 Jun 29;10(7):1049. PMID: 34209813
Free Radic Biol Med

Decreased expression of IDH1 by chronic unpredictable stress suppresses proliferation and accelerates senescence of granulosa cells through ROS activated MAPK signaling pathways. [Abstract]

2021 Jun:169:122-136. PMID: 33865962
Cell Rep

IFNα-induced BST2+ tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling. [Abstract]

2024 Apr 23;43(4):114088. PMID: 38602878
Curr Res Food Sci

Apigenin and its octoic acid diester attenuated glycidol-induced autophagic-dependent apoptosis via inhibiting the ERK/JNK/p38 signaling pathways in human umbilical vein endothelial cells (HUVECs). [Abstract]

2023 Jan 16:6:100447. PMID: 36699118
Cell Prolif

ERK/Drp1-dependent mitochondrial fission contributes to HMGB1-induced autophagy in pulmonary arterial hypertension. [Abstract]

2021 Jun;54(6):e13048. PMID: 33948998
J Ginseng Res

2026 Jul 21.
Br J Pharmacol

Sevoflurane protects against testicular ischaemia-reperfusion injury in rats. [Abstract]

2026 Mar 24. PMID: 41877502
J Pineal Res

Melatonin promotes the proliferation of primordial germ cell-like cells derived from porcine skin-derived stem cells: A mechanistic analysis. [Abstract]

2022 Nov;73(4):e12833. PMID: 36106819
Chin Med

Ferulic acid in combination with ginsenoside Rb1 alleviates myocardial no-reflow by inhibiting platelet HMGB1 release and NET formation. [Abstract]

2026 Jan 8;21(1):16. PMID: 41501834
Clin Sci

NELL2 modulates cell proliferation and apoptosis via ERK pathway in the development of benign prostatic hyperplasia. [Abstract]

2021 Jul 16;135(13):1591-1608. PMID: 34195782
J Invest Dermatol

Advanced Glycation End Products Promote Melanogenesis by Activating NLRP3 Inflammasome in Human Dermal Fibroblasts. [Abstract]

2022 Oct;142(10):2591-2602.e8. PMID: 35421403
Front Immunol

Macrophage Polarization Modulated by Porcine Circovirus Type 2 Facilitates Bacterial Coinfection. [Abstract]

2021 Jul 28:12:688294. PMID: 34394082
Neurosci Bull

IL-33 Regulates the Phenotypic Transformation of Reactive Astrocytes via PENK-ERK/MAPK Pathway in Parkinson's Disease. [Abstract]

2026 Jan 3. PMID: 41483148
JCI Insight

Injury-induced FoxM1 expression in mouse kidney drives epithelial proliferation by a Cyclin F dependent mechanism. [Abstract]

2024 Jun 25;9(15):e175416. PMID: 38916959
JCI Insight

FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis. [Abstract]

2022 May 23;7(10):e157874. PMID: 35439170
J Agric Food Chem

Molecular Mechanism Study of Dietary Fiber Lignin Intervention in Ulcerative Colitis through GPR37. [Abstract]

2024 Jun 7. PMID: 38845410
Ecotoxicol Environ Saf

Chrysene contribution to bronchial asthma: Activation of TRPA1 disrupts bronchial epithelial barrier via ERK pathway. [Abstract]

2024 Nov 1:286:117095. PMID: 39395376
Biochem Pharmacol

Bosentan confers cardioprotection against cisplatin toxicity: Involvement of β-arrestin-linked ETA receptor signaling. [Abstract]

2026 Aug;250(Pt 2):118027. PMID: 42069228
Biochem Pharmacol

Tirzepatide ameliorates cisplatin-induced acute kidney injury by restoring NAMPT/NAD + homeostasis and enhancing Pink1-Parkin-mediated mitophagy. [Abstract]

2026 Aug;250(Pt 2):118040. PMID: 42114682
Biochem Pharmacol

Targeting neuropilin-1 attenuates 4-hydroxytamoxifen-induced stemness and sensitizes ERα-re-expressing TNBC to tamoxifen. [Abstract]

2026 Jun 12;251(Pt 2):118163. PMID: 42285368
Cell Biosci

LBH deficiency influences the therapeutic effects of regulatory B cells through the regulation of IL-10 production via the Rap1 pathway. [Abstract]

2026 May 24. PMID: 42178582
Biochem Pharmacol

Jolkinolide B synergistically potentiates the antitumor activity of GPX4 inhibitors via inhibiting TrxR1 in cisplatin-resistant bladder cancer cells. [Abstract]

2024 May:223:116194. PMID: 38583812
Biochem Pharmacol

Downregulation of PDZK1 by TGF-β1 promotes renal fibrosis via inducing epithelial-mesenchymal transition of renal tubular cells. [Abstract]

2024 Feb:220:116015. PMID: 38158021
Cell Mol Life Sci

Cordycepin delays postovulatory aging of oocytes through inhibition of maternal mRNAs degradation via DCP1A polyadenylation suppression. [Abstract]

2023 Nov 25;80(12):372. PMID: 38001238
Biochem Pharmacol

Differential regulation of histamine H1 receptor-mediated ERK phosphorylation by Gq proteins and arrestins. [Abstract]

2023 Jul:213:115595. PMID: 37201878
Biochem Pharmacol

Lenvatinib inhibited HCC cell migration and invasion through regulating the transcription and ubiquitination of UHRF1 and DNMT1. [Abstract]

2023 Apr:210:115489. PMID: 36893815

U0126 purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2023 Apr:210:115489. [Abstract]

U0126 (20 uM; pretreat for 2 h) rescues the loss of UHRF1/DNMT1 expression and the increase of E-Cadherin expression that induced by Lenvatinib in Huh7 cells.
Cell Mol Life Sci

TGF-β1 promotes epithelial-to-mesenchymal transition and stemness of prostate cancer cells by inducing PCBP1 degradation and alternative splicing of CD44. [Abstract]

2021 Feb;78(3):949-962. PMID: 32440711
Food Funct

Genistein suppresses allergic contact dermatitis through regulating the MAP2K2/ERK pathway. [Abstract]

2021 May 21;12(10):4556-4569. PMID: 33908440
Drug Des Devel Ther

Design, Synthesis, and Biological Evaluation of Novel Hydroxytyrosol Derivatives as Protectors for Vascular Endothelium Against Lipid Overload. [Abstract]

2025 Apr 1:19:2433-2452. PMID: 40190807
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Aquaculture

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Endocr Relat Cancer

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bioRxiv

2026 Jan 24.
Hong Kong University of Science and Technology

2025.
bioRxiv

2025 Sep 29.
bioRxiv

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Research Square Preprint

2024 Apr 19.
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SSRN

2023 Sep 18.
Research Square Preprint

2023 May 31.
bioRxiv

2023 Apr 18.
Oxid Med Cell Longev

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2022 Mar 10:2022:1863098. PMID: 35368866
Research Square Preprint

2021 Nov.
Research Square Preprint

2021 Apr.
Research Square Preprint

2021 Feb.
Biomed Pharmacother

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Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (262.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6282 mL

13.1409 mL

26.2819 mL

5 mM

0.5256 mL

2.6282 mL

5.2564 mL

10 mM

0.2628 mL

1.3141 mL

2.6282 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Molecular Weight Molecular Weight

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Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.57 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Favata MF, et al. Identification of a novel inhibitor of mitogen-activated protein kinase kinase. J Biol Chem. 1998 Jul 17;273(29):18623-32. [Content Brief]

[2]. Droebner K, et al. Antiviral activity of the MEK-inhibitor U0126 against pandemic H1N1v and highly pathogenic avian influenza virus in vitro and in vivo. Antiviral Res. 2011, 92(2), 195-203. [Content Brief]

[3]. Bessard A, et al. RNAi-mediated ERK2 knockdown inhibits growth of tumor cells in vitro and in vivo. Oncogene. 2008 Sep 11;27(40):5315-25. [Content Brief]

[4]. Ahnstedt H, et al. U0126 attenuates cerebral vasoconstriction and improves long-term neurologic outcome after stroke in female rats. J Cereb Blood Flow Metab. 2015 Mar;35(3):454-60. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6282 mL	13.1410 mL	26.2819 mL	65.7048 mL
	5 mM	0.5256 mL	2.6282 mL	5.2564 mL	13.1410 mL
	10 mM	0.2628 mL	1.3141 mL	2.6282 mL	6.5705 mL
	15 mM	0.1752 mL	0.8761 mL	1.7521 mL	4.3803 mL
	20 mM	0.1314 mL	0.6570 mL	1.3141 mL	3.2852 mL
	25 mM	0.1051 mL	0.5256 mL	1.0513 mL	2.6282 mL
	30 mM	0.0876 mL	0.4380 mL	0.8761 mL	2.1902 mL
	40 mM	0.0657 mL	0.3285 mL	0.6570 mL	1.6426 mL
	50 mM	0.0526 mL	0.2628 mL	0.5256 mL	1.3141 mL
	60 mM	0.0438 mL	0.2190 mL	0.4380 mL	1.0951 mL
	80 mM	0.0329 mL	0.1643 mL	0.3285 mL	0.8213 mL
	100 mM	0.0263 mL	0.1314 mL	0.2628 mL	0.6570 mL

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U0126

Publications Citing Use of MedChemExpress (MCE) U0126

All MEK Isoforms