BRD4-IN-4
Based on 1 Customer Validation
BRD4-IN-4 (Compound 1) is a BRD4 inhibitor (IC50=6.83 μM). BRD4-IN-4 selectively inhibits MV4-11 cell line proliferation and arrests cell at G1 phase. BRD4-IN-4 can be used for research of MLL leukemia.
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- Purity : 96.0%
- CAS No.: 304685-40-3
- 화학식: C17H18N2O3S
- 분자량:330.40
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보관:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
IC50 & Target
IC50: 6.83 μM (BRD4)[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MV4-11 | IC50 |
11.67 μM
Compound: 1
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Antiproliferative activity against human MV4-11 cells after 72 hrs by CellTiter-Glo luminescent assay
Antiproliferative activity against human MV4-11 cells after 72 hrs by CellTiter-Glo luminescent assay
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[PMID: 29730189] |
Chemical Information
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CAS No. 304685-40-3
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Appearance Solid
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분자량 330.40
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화학식 C17H18N2O3S
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Color Brown to reddish brown
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SMILES
O=C1C2=CC=CC3=C(S(=O)(N4CCCC4)=O)C=CC(N1CC)=C32
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
용액&용해도
In Vitro:
DMSO : 100 mg/mL (302.66 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
순도&문서
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0266 mL | 15.1332 mL | 30.2663 mL | 75.6659 mL |
| 5 mM | 0.6053 mL | 3.0266 mL | 6.0533 mL | 15.1332 mL | |
| 10 mM | 0.3027 mL | 1.5133 mL | 3.0266 mL | 7.5666 mL | |
| 15 mM | 0.2018 mL | 1.0089 mL | 2.0178 mL | 5.0444 mL | |
| 20 mM | 0.1513 mL | 0.7567 mL | 1.5133 mL | 3.7833 mL | |
| 25 mM | 0.1211 mL | 0.6053 mL | 1.2107 mL | 3.0266 mL | |
| 30 mM | 0.1009 mL | 0.5044 mL | 1.0089 mL | 2.5222 mL | |
| 40 mM | 0.0757 mL | 0.3783 mL | 0.7567 mL | 1.8916 mL | |
| 50 mM | 0.0605 mL | 0.3027 mL | 0.6053 mL | 1.5133 mL | |
| 60 mM | 0.0504 mL | 0.2522 mL | 0.5044 mL | 1.2611 mL | |
| 80 mM | 0.0378 mL | 0.1892 mL | 0.3783 mL | 0.9458 mL | |
| 100 mM | 0.0303 mL | 0.1513 mL | 0.3027 mL | 0.7567 mL |
BRD4-IN-4 Related Classifications
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