Bromotubercidin
Based on 1 Customer Validation
Bromotubercidin is a RNA synthesis inhibitor. Bromotubercidin induces biphasic decreases in vasopressin biosynthesis and causes progressive reduction in vasopressin biosynthesis.
For research use only. We do not sell to patients.
- CAS No.: 21193-80-6
- Formula: C11H13BrN4O4
- Molecular Weight:345.15
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HFF | IC50 |
1.2 μM
Compound: 1b
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Tested for cytotoxicity in human foreskin fibroblasts at time of HCMV plaque enumeration
Tested for cytotoxicity in human foreskin fibroblasts at time of HCMV plaque enumeration
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[PMID: 8254613] |
| HFF | IC50 |
4.5 μM
Compound: 1n
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Cytotoxicity against uninfected human foreskin fibroblast(HFF cells)
Cytotoxicity against uninfected human foreskin fibroblast(HFF cells)
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[PMID: 2846837] |
| HFF | IC50 |
4.5 μM
Compound: Br-Tub
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Cytotoxic activity against HFF cells.
Cytotoxic activity against HFF cells.
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[PMID: 2163454] |
| KB | IC50 |
2.3 μM
Compound: 1n
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Cytotoxicity against human neoplastic cell line(KB cells)
Cytotoxicity against human neoplastic cell line(KB cells)
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[PMID: 2846837] |
| KB | IC50 |
3 μM
Compound: 1b
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Tested for the inhibition of KB cell growth
Tested for the inhibition of KB cell growth
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[PMID: 8254613] |
| KB | IC50 |
3 μM
Compound: 5-bromotubercidin
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Cytotoxicity of compound was determined by assaying cell growth in human neoplastic cell line(KB).
Cytotoxicity of compound was determined by assaying cell growth in human neoplastic cell line(KB).
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[PMID: 1310744] |
| L1210 | IC50 |
4 μM
Compound: 1b
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Tested for in vitro cell growth inhibition of L1210 cells
Tested for in vitro cell growth inhibition of L1210 cells
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[PMID: 8254613] |
| L1210 | IC50 |
4 μM
Compound: 5-bromotubercidin
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Concentration required to decrease the growth rate to 50% of control was evaluated by determining their ability to inhibit growth of L1210 cells in vitro.
Concentration required to decrease the growth rate to 50% of control was evaluated by determining their ability to inhibit growth of L1210 cells in vitro.
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[PMID: 1310744] |
| L1210 | IC50 |
4 μM
Compound: Br-Tub
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In vitro inhibition of L1210 cell growth to half of the control rate
In vitro inhibition of L1210 cell growth to half of the control rate
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[PMID: 2163454] |
| Neoplastic cell line | IC50 |
2.3 μM
Compound: Br-Tub
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Cytotoxic activity against KB cells.
Cytotoxic activity against KB cells.
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[PMID: 2163454] |
Chemical Information
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CAS No. 21193-80-6
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Appearance Solid
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Molecular Weight 345.15
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Formula C11H13BrN4O4
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SMILES
O[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(N)=C3C(Br)=C2)O[C@@H]1CO
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (289.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.24 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.24 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8973 mL | 14.4865 mL | 28.9729 mL | 72.4323 mL |
| 5 mM | 0.5795 mL | 2.8973 mL | 5.7946 mL | 14.4865 mL | |
| 10 mM | 0.2897 mL | 1.4486 mL | 2.8973 mL | 7.2432 mL | |
| 15 mM | 0.1932 mL | 0.9658 mL | 1.9315 mL | 4.8288 mL | |
| 20 mM | 0.1449 mL | 0.7243 mL | 1.4486 mL | 3.6216 mL | |
| 25 mM | 0.1159 mL | 0.5795 mL | 1.1589 mL | 2.8973 mL | |
| 30 mM | 0.0966 mL | 0.4829 mL | 0.9658 mL | 2.4144 mL | |
| 40 mM | 0.0724 mL | 0.3622 mL | 0.7243 mL | 1.8108 mL | |
| 50 mM | 0.0579 mL | 0.2897 mL | 0.5795 mL | 1.4486 mL | |
| 60 mM | 0.0483 mL | 0.2414 mL | 0.4829 mL | 1.2072 mL | |
| 80 mM | 0.0362 mL | 0.1811 mL | 0.3622 mL | 0.9054 mL | |
| 100 mM | 0.0290 mL | 0.1449 mL | 0.2897 mL | 0.7243 mL |
Bromotubercidin Related Classifications
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