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Cat. No.: HY-182293

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BTK-IN-48

BTK-IN-48 is a BTK inhibitor with an IC₅₀ of 1.14 μM. BTK-IN-48 inhibits recombinant BTK and c-Src, and exerts moderate inhibitory effects on LCK, BMX/ETK, FLT3 and PIM1. BTK-IN-48 can be used in the research of B-cell malignancies and autoimmune diseases.

For research use only. We do not sell to patients.

CAS No.: 3114078-55-3
Formula: C₁₉H₁₅N₃O₃
Molecular Weight:333.34

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

BTK-IN-48 Chemical Structure

CAS No.: 3114078-55-3

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Src Isoforms :

All Pim Isoforms :

In Vitro

BTK-IN-48 (Compound 6b) potently inhibits recombinant BTK with an IC₅₀ of 1.14 μM, and achieves an inhibition rate of 88.6% at a single concentration of 10 μM^[1].
BTK-IN-48 (10 μM) potently inhibits recombinant BTK and c-Src at a concentration of 10 μM, and exerts moderate inhibitory effects on LCK, BMX/ETK, FLT3 and PIM1^[1].
BTK-IN-48 (0-40 μM; 4 days) exerts mild growth inhibition on RPMI-1788 and RPMI-7666 normal B cells, but does not induce significant cell death^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

RPMI-1788, RPMI-7666 cells
Concentration:

0, 10, 20, 30, 40 μM
Incubation Time:

4 days
Result:

Reduced viability of RPMI-1788 cells to ~60% of untreated controls at 40 μM.
Reduced viability of RPMI-7666 cells to ~85% of untreated controls at 40 μM.
Did not cause significant increase in ToPro3-positive (dead) cells across all tested concentrations, with cell death remaining below 5% for both cell lines.

Chemical Information

CAS No. 3114078-55-3
Molecular Weight 333.34
Formula C₁₉H₁₅N₃O₃
SMILES

COC(C=C1)=CC=C1N(C(C2=CC=C(O)C=C2O)=N3)C4=C3C=CC=N4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Kim Y, et al. Discovery of novel imidazo[4,5-b]pyridine derivatives as noncovalent reversible Bruton's tyrosine kinase inhibitors. Bioorg Med Chem Lett. 2026 Mar;132:130497. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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Biological Activity
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BTK-IN-48

All Src Isoforms

All Pim Isoforms

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

BTK-IN-48 Related Classifications

HY-182293 Related Classifications