Aristeromycin
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Aristeromycin, an adenosine analog, is an antibiotic and a potent S-adenosylhomocysteine hydrolase (AHCY) inhibitor.
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- Pureté: 99.10%
- CAS No.: 19186-33-5
- Formule: C11H15N5O3
- Masse moléculaire:265.27
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
S-adenosylhomocysteine hydrolase[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| P388 | IC50 |
0.5 μg/mL
Compound: C-Ado
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Cytotoxicity against P388 mouse leukemia cells
Cytotoxicity against P388 mouse leukemia cells
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[PMID: 1433208] |
| Vero | CC50 |
2 μM
Compound: 1
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Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability measured after 72 hrs by MTS assay
Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability measured after 72 hrs by MTS assay
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[PMID: 31244113] |
| Vero | CC50 |
2.4 μM
Compound: 1
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Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability measured after 96 hrs by MTS assay
Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability measured after 96 hrs by MTS assay
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[PMID: 31244113] |
| Vero | EC50 |
>50 μM
Compound: 1
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Inhibition of RNA-dependent RNA polymerase in MERS-CoV EMC/2012 infected in African green monkey Vero cells assessed as reduction in virus-induced cytopathic effect measured after 72 hrs post-infection by MTS assay
Inhibition of RNA-dependent RNA polymerase in MERS-CoV EMC/2012 infected in African green monkey Vero cells assessed as reduction in virus-induced cytopathic effect measured after 72 hrs post-infection by MTS assay
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[PMID: 31244113] |
| Vero | EC50 |
0.64 μM
Compound: 1
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Inhibition of RNA-dependent RNA polymerase in ZIKV SL0612 infected in African green monkey Vero cells assessed as reduction in virus-induced cytopathic effect measured after 96 hrs post-infection by MTS assay
Inhibition of RNA-dependent RNA polymerase in ZIKV SL0612 infected in African green monkey Vero cells assessed as reduction in virus-induced cytopathic effect measured after 96 hrs post-infection by MTS assay
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[PMID: 31244113] |
| Vero C1008 | EC50 |
>50 μM
Compound: 1
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Inhibition of RNA-dependent RNA polymerase in SARS-CoV Frankfurt1 infected in African green monkey Vero E6 cells assessed as reduction in virus-induced cytopathic effect measured after 72 hrs post-infection by MTS assay
Inhibition of RNA-dependent RNA polymerase in SARS-CoV Frankfurt1 infected in African green monkey Vero E6 cells assessed as reduction in virus-induced cytopathic effect measured after 72 hrs post-infection by MTS assay
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[PMID: 31244113] |
| Vero C1008 | EC50 |
0.8 μM
Compound: 1
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Inhibition of RNA-dependent RNA polymerase in CHIKV LS3 infected in African green monkey Vero E6 cells assessed as reduction in virus-induced cytopathic effect measured after 96 hrs post-infection by MTS assay
Inhibition of RNA-dependent RNA polymerase in CHIKV LS3 infected in African green monkey Vero E6 cells assessed as reduction in virus-induced cytopathic effect measured after 96 hrs post-infection by MTS assay
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[PMID: 31244113] |
| Vero C1008 | CC50 |
≥2.4 μM
Compound: 1
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Cytotoxicity against African green monkey Vero E6 cells assessed as reduction in cell viability measured after 72 hrs by MTS assay
Cytotoxicity against African green monkey Vero E6 cells assessed as reduction in cell viability measured after 72 hrs by MTS assay
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[PMID: 31244113] |
The IC50 value of Aristeromycin against AHCY is 38.5 nM at 50 μM S-adenosylhomocysteine (SAH) (approximately equal to the Km: 48 μM), but 271 nM at 1000 μM SAH (20× Km). With 60 min of preincubation, the mean IC50 value of Aristeromycin at 50 μM SAH is 12.7 nM[1].
Aristeromycin has IC50 values of 3.2 μM for LNCaP-FGC cell growth and 0.88 μM for LNCaP-hr cell growth[1].
At least in part, Aristeromycin can regulate oncogenic EZH2 expression by inducing miR-26a[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 19186-33-5
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Appearance Solid
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Masse moléculaire 265.27
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Formule C11H15N5O3
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Color White to off-white
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SMILES
O[C@@H]1[C@H](O)[C@@H](CO)C[C@H]1N2C3=NC=NC(N)=C3N=C2
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Structure Classification
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Initial Source
S. citricolor
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
DMSO : 50 mg/mL (188.49 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (9.42 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Uchiyama N, et al. Aristeromycin and DZNeP cause growth inhibition of prostate cancer via induction of mir-26a. Eur J Pharmacol. 2017 Oct 5;812:138-146. [Content Brief]
[2]. Ishikura T, et al. Inhibition of S-adenosylhomocysteine hydrolase by purine nucleoside analogues. Nucleic Acids Symp Ser. 1983;(12):119-22. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.7697 mL | 18.8487 mL | 37.6974 mL | 94.2436 mL |
| 5 mM | 0.7539 mL | 3.7697 mL | 7.5395 mL | 18.8487 mL | |
| 10 mM | 0.3770 mL | 1.8849 mL | 3.7697 mL | 9.4244 mL | |
| 15 mM | 0.2513 mL | 1.2566 mL | 2.5132 mL | 6.2829 mL | |
| 20 mM | 0.1885 mL | 0.9424 mL | 1.8849 mL | 4.7122 mL | |
| 25 mM | 0.1508 mL | 0.7539 mL | 1.5079 mL | 3.7697 mL | |
| 30 mM | 0.1257 mL | 0.6283 mL | 1.2566 mL | 3.1415 mL | |
| 40 mM | 0.0942 mL | 0.4712 mL | 0.9424 mL | 2.3561 mL | |
| 50 mM | 0.0754 mL | 0.3770 mL | 0.7539 mL | 1.8849 mL | |
| 60 mM | 0.0628 mL | 0.3141 mL | 0.6283 mL | 1.5707 mL | |
| 80 mM | 0.0471 mL | 0.2356 mL | 0.4712 mL | 1.1780 mL | |
| 100 mM | 0.0377 mL | 0.1885 mL | 0.3770 mL | 0.9424 mL |
Aristeromycin Related Classifications
HY-112639 Related Classifications
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