HJC0152
Based on 3 publication(s) in Google Scholar
HJC0152 is a signal transducers and activators of transcription 3 (STAT3) inhibitor.
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- Pureté : 99.75%
- CAS No.: 1420290-99-8
- Formule: C15H14Cl3N3O4
- Masse moléculaire:406.65
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Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) HJC0152
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Customer Validation & Images
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WB
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IF
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RT-PCR
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In Vivo Efficacy Study
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Histological Imaging/Staining
Activité biologique
Description
IC50 & Target
[1]|
STAT3 |
In Vitro
HJC0152 (compound 11) significantly inhibits cell proliferation and induces apoptosis accompanying cellular morphological changes at concentrations of 1, 5, and 10 μM. Results show that treatment with 10 μM HJC0152 decreases the STAT3 promoter activity in MDA-MB-231 cells by approximately 32%, and increasing the dose of HJC0152 to 20 μM further decreases STAT3 promoter activity by 62% as compare with control. Total STAT3 is reduced after treatment with HJC0152. HJC0152 induces cleaved caspase-3 and down regulates cyclin D1 in MDA-MB-231 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1420290-99-8
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Appearance Solid
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Masse moléculaire 406.65
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Formule C15H14Cl3N3O4
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Color White to yellow
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SMILES
O=C(NC1=CC=C([N+]([O-])=O)C=C1Cl)C2=CC(Cl)=CC=C2OCCN.[H]Cl
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (3)
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Journal Impact Factor
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Most Recent
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Int J Mol Sci
STAT3 Increases CVB3 Replication and Acute Pancreatitis and Myocarditis Pathology via Impeding Nuclear Translocation of STAT1 and Interferon-Stimulated Gene Expression. [Abstract]2024 Aug 19;25(16):9007. PMID: 39201692
HJC0152 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Aug 19;25(16):9007. [Abstract]
Nucleic or cytoplasmic p-STAT1 and STAT1 expressions were analyzed by immunoblotting analysis treated with HJC0152 (10 μM) for 48 h.
HJC0152 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Aug 19;25(16):9007. [Abstract]
IF assay to analyze nuclear translocation of p-STAT1 in STAT3 inhibitor-treated cells. Acinar 266-6 cells were mock-infected or infected with CVB3 and fixed 12 h p.i. The p-STAT1 expression and localizations were analyzed by confocal microscopy treated with HJC0152 (10 μM) for 48 h.
HJC0152 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Aug 19;25(16):9007. [Abstract]
mRNA levels of ISGs (OAS, MXA, ISG15, ISG56, Viperin and CXCL10 and GAPDH) from HJC0152 (10 μM)-treated cells were analyzed by RT-QPCR.
HJC0152 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Aug 19;25(16):9007. [Abstract]
Survival curve and weight loss curve in STAT3 inhibitor (HJC0152) (12.5 mg/kg, i.p.)-treated mice were followed by 7 dpi.
HJC0152 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Aug 19;25(16):9007. [Abstract]
Representative hematoxylin–eosin (H&E)-stained pancreas and heart sections from infected mice treated with HJC0152 (12.5 mg/kg, i.p.).
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Fish Shellfish Immunol
Butyrate induces STAT3/HIF-1α/IL-22 signaling via GPCR and HDAC3 inhibition to activate autophagy in head kidney macrophages from turbot (Scophthalmus maximus L.). [Abstract]2023 Dec:143:109214. PMID: 37977544 -
Naunyn Schmiedebergs Arch Pharmacol
Corynoline promotes apoptosis and inhibits proliferation of glioblastoma via regulating the STAT3/Bcl-2 signalling pathway. [Abstract]2025 Dec 11. PMID: 41379317
Solvant et solubilité
In Vitro:
DMSO : 20 mg/mL (49.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (4.92 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2 mg/mL (4.92 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 10 mg/mL (24.59 mM); Suspended solution; Need ultrasonic
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4591 mL | 12.2956 mL | 24.5912 mL | 61.4779 mL |
| 5 mM | 0.4918 mL | 2.4591 mL | 4.9182 mL | 12.2956 mL | |
| 10 mM | 0.2459 mL | 1.2296 mL | 2.4591 mL | 6.1478 mL | |
| 15 mM | 0.1639 mL | 0.8197 mL | 1.6394 mL | 4.0985 mL | |
| 20 mM | 0.1230 mL | 0.6148 mL | 1.2296 mL | 3.0739 mL | |
| 25 mM | 0.0984 mL | 0.4918 mL | 0.9836 mL | 2.4591 mL | |
| 30 mM | 0.0820 mL | 0.4099 mL | 0.8197 mL | 2.0493 mL | |
| 40 mM | 0.0615 mL | 0.3074 mL | 0.6148 mL | 1.5369 mL |
HJC0152 Related Classifications
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