USP7-IN-9
Based on 1 Customer Validation
USP7-IN-9 is a highly potent ubiquitin-specific protease 7 (USP7) inhibitor with an IC50 value of 40.8 nM. USP7-IN-9 can induce apoptosis and arrest cell progression at G0/G1 and S phases in RS4; 11 cells. USP7-IN-9 reduces the protein levels of oncoproteins MDM2 and DNMT1 and increases the protein levels of tumor suppressors p53 and p21.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté: 98.10%
- CAS No.: 2444374-01-8
- Formule: C32H33ClF6N6O8
- Masse moléculaire:779.08
-
Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
-
Solvant et solubilité
- Pureté et documentation
- Références
-
Help & FAQs
-
Apoptosis Compound Library
HY-L003
-
Cell Cycle/DNA Damage Compound Library
HY-L004
-
Anti-Cancer Compound Library
HY-L025
-
Anti-Aging Compound Library
HY-L034
-
Ubiquitination Compound Library
HY-L050
-
Heterocyclic Compound Library
HY-L138
-
Metabolic Enzyme Compound Library
HY-L146
-
Protease Inhibitor Library
HY-L147
-
Highly Selective Inhibitors Library
HY-L158
-
Highly Selective Activators Library
HY-L159
-
Cell Death Library
HY-L162
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Bioactive Compound Library
HY-L001
-
Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
-
Cell Proliferation Compound Library
HY-L201
-
High-Throughput Bioactive Compound Library
HY-L205
-
Posttranslational Modification Library
HY-L226
Activité biologique
IC50: 40.8 nM (USP7)[1]
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
32.8 μM
Compound: L55
|
Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 3 days by cell-titer glo assay
Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 3 days by cell-titer glo assay
|
[PMID: 32497973] |
| Huh-7 | IC50 |
3.6 μM
Compound: L55
|
Antiproliferative activity against human HuH-7 cells assessed as reduction in cell viability incubated for 3 days by cell-titer glo assay
Antiproliferative activity against human HuH-7 cells assessed as reduction in cell viability incubated for 3 days by cell-titer glo assay
|
[PMID: 32497973] |
| LNCaP | IC50 |
29.6 nM
Compound: L55
|
Antiproliferative activity against human LNCaP cells assessed as reduction in cell viability incubated for 6 days by cell-titer glo assay
Antiproliferative activity against human LNCaP cells assessed as reduction in cell viability incubated for 6 days by cell-titer glo assay
|
[PMID: 32497973] |
| NB-4 | IC50 |
5 μM
Compound: L55
|
Antiproliferative activity against human NB-4 cells assessed as reduction in cell viability incubated for 3 days by cell-titer glo assay
Antiproliferative activity against human NB-4 cells assessed as reduction in cell viability incubated for 3 days by cell-titer glo assay
|
[PMID: 32497973] |
| RS4-11 | IC50 |
41.6 nM
Compound: L55
|
Antiproliferative activity against human RS4-11 cells assessed as reduction in cell viability incubated for 3 days by cell-titer glo assay
Antiproliferative activity against human RS4-11 cells assessed as reduction in cell viability incubated for 3 days by cell-titer glo assay
|
[PMID: 32497973] |
USP7-IN-9 (compound L55) (0-50 μM; 3 or 6 days) exhibits inhibitory activity against cancer cells[1].
USP7-IN-9 (1 μM; 0-72 hours) reduces the proportions of G2/M cells, and does not change the proportions of G0/G1 and S cells[1].
USP7-IN-9 (0.1-1 μM; 24 hours) reduces the protein levels of MDM2 and DNMT1 in a dose-dependent manner, and increases the protein levels of p53 and p21[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:LNCaP, RS4; 11, HCT 116, NB4, K562 and HuH-7 cells[1]
-
Concentration:0-50 μM
-
Incubation Time:LNCaP, 6 days; RS4; 11, HCT 116, HuH-7, K562 and NB4, 3 days
-
Result:Exhibited high inhibitory activity against LNCaP and RS4; 11 cells, with IC50s of 29.6 nM and 41.6 nM, respectively, and weak inhibitory activity on HCT 116, NB4, K562 and HuH-7 cells.
-
Cell Line:RS4; 11 cells[1]
-
Concentration:1 μM
-
Incubation Time:0, 24, 48 and 72 hours
-
Result:Reduced the proportions of G2/M cells, while the proportions of G0/G1 and S cells were not apparently altered.
-
Cell Line:RS4; 11 cells[1]
-
Concentration:0.1, 0.3 and 1 μM
-
Incubation Time:24 hours
-
Result:Reduced the protein levels of MDM2 and DNMT1 in a dose-dependent manner, and increased the protein levels of p53 and p21.
Chemical Information
-
CAS No. 2444374-01-8
-
Appearance Solid
-
Masse moléculaire 779.08
-
Formule C32H33ClF6N6O8
-
Color Light yellow to yellow
-
SMILES
OC1(CN(C=NC2=C(C3=CC=C(C[NH3+])C=C3)N(C)N=C24)C4=O)CC[NH+](CC5=CC=C(C(OC)=O)C=C5Cl)CC1.O=C([O-])C(F)(F)F.O=C([O-])C(F)(F)F
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvant et solubilité
DMSO : 100 mg/mL (128.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
-
Fiche technique (273 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2836 mL | 6.4178 mL | 12.8357 mL | 32.0891 mL |
| 5 mM | 0.2567 mL | 1.2836 mL | 2.5671 mL | 6.4178 mL | |
| 10 mM | 0.1284 mL | 0.6418 mL | 1.2836 mL | 3.2089 mL | |
| 15 mM | 0.0856 mL | 0.4279 mL | 0.8557 mL | 2.1393 mL | |
| 20 mM | 0.0642 mL | 0.3209 mL | 0.6418 mL | 1.6045 mL | |
| 25 mM | 0.0513 mL | 0.2567 mL | 0.5134 mL | 1.2836 mL | |
| 30 mM | 0.0428 mL | 0.2139 mL | 0.4279 mL | 1.0696 mL | |
| 40 mM | 0.0321 mL | 0.1604 mL | 0.3209 mL | 0.8022 mL | |
| 50 mM | 0.0257 mL | 0.1284 mL | 0.2567 mL | 0.6418 mL | |
| 60 mM | 0.0214 mL | 0.1070 mL | 0.2139 mL | 0.5348 mL | |
| 80 mM | 0.0160 mL | 0.0802 mL | 0.1604 mL | 0.4011 mL | |
| 100 mM | 0.0128 mL | 0.0642 mL | 0.1284 mL | 0.3209 mL |
USP7-IN-9 Related Classifications
HY-146887 Related Classifications
Powered by Bioz