Sirtinol
Based on 14 publication(s) in Google Scholar
Sirtinol is a sirtuin (SIRT) inhibitor, with IC50s of 48 μM, 57.7 μM and 131 μM for ySir2, hSIRT2 and hSIRT2, respectively.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 98.0%
- CAS. Nr.: 410536-97-9
- Formel: C26H22N2O2
- Molecular Weight:394.47
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
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Publications Citing Use of MedChemExpress (MCE) Sirtinol
More- Cell Death Dis. 2018 May 1;9(5):559. [Abstract]
- Acta Pharmacol Sin. 2021 Nov;42(11):1834-1846. [Abstract]
- Neural Regen Res. 2021 Dec;16(12):2465-2474. [Abstract]
- Life Sci. 2021 Jun 1:274:119299. [Abstract]
- J Cell Mol Med. 2020 Sep;24(17):10027-10041. [Abstract]
- J Funct Foods. November 2021, 104686.
- Diabetol Metab Syndr. 2025 Nov 14;17(1):427. [Abstract]
- Mol Cell Biochem. 2018 Feb;439(1-2):213-223. [Abstract]
- Cell Cycle. 2020 Sep;19(17):2216-2225. [Abstract]
- Acta Histochem. 2022 Dec 16;125(1):151989. [Abstract]
- Graefes Arch Clin Exp Ophthalmol. 2020 Feb;258(2):335-344. [Abstract]
- Acta Cardiol Sin. 2024 Mar;40(2):225-234. [Abstract]
- Research Square Print. 2022 Jul.
- Research Square Preprint. 2020 Dec.
Customer Validation & Images
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RT-PCR
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WB
Biologische Aktivität
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ySir2 48 μM (IC50) |
hSIRT2 57.7 μM (IC50) |
hSIRT1 131 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
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| Fibroblast | IC50 |
>25 μM
Compound: Sirtinol
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Toxicity in neonatal foreskin fibroblasts
Toxicity in neonatal foreskin fibroblasts
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[PMID: 18212103] |
| Hs 683 | IC50 |
33.9 μM
Compound: 2
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Antiproliferative activity against human Hs683 cells after 72 hrs by MTT assay
Antiproliferative activity against human Hs683 cells after 72 hrs by MTT assay
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[PMID: 28475330] |
| Neutrophil | IC50 |
>10 μM
Compound: 1
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Inhibition of O2 generation in fMLP/CB-induced human neutrophils by cell based assay
Inhibition of O2 generation in fMLP/CB-induced human neutrophils by cell based assay
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[PMID: 37939864] |
| Neutrophil | IC50 |
1.92 μM
Compound: 1
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Inhibition of elastase release in fMLP/CB-induced human neutrophils by cell based assay
Inhibition of elastase release in fMLP/CB-induced human neutrophils by cell based assay
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[PMID: 37939864] |
| U-373MG ATCC | IC50 |
39.3 μM
Compound: 2
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Antiproliferative activity against human U373 cells after 72 hrs by MTT assay
Antiproliferative activity against human U373 cells after 72 hrs by MTT assay
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[PMID: 28475330] |
Sirtinol reduces the growth of MCF-7 cells in a concentration- and time-dependent manner. The IC50 values of sirtinol are 48.6 μM and 43.5 μM after 24 and 48 h of treatment, respectively. Sirtinol significantly decreases SIRT1 expression and increases the acetylated p53 level[1]. Sirtinol attenuates the proliferation and induces apoptosis of nonsmall cell lung cancer (NSCLC) H1299 cells and causes the significantly increased level of FoxO3a, a proapoptotic transcription factor targeted by Sirt1[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 410536-97-9
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Appearance Solid
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Molecular Weight 394.47
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Formel C26H22N2O2
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Color Light yellow to yellow
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SMILES
O=C(NC(C1=CC=CC=C1)C)C2=CC=CC=C2/N=C/C3=C4C=CC=CC4=CC=C3O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (14)
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Journal Impact Factor
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Most Recent
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Cell Death Dis
Checkpoint suppressor 1 suppresses transcriptional activity of ERα and breast cancer cell proliferation via deacetylase SIRT1. [Abstract]2018 May 1;9(5):559. PMID: 29752474
Sirtinol purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 May 1;9(5):559. [Abstract]
The inhibitory effect of CHES1 on transcription activity of ERα Is diminished in MCF7 cells when treated with Sirtinol (the SIRT1 inhibitor).
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Acta Pharmacol Sin
SL010110, a lead compound, inhibits gluconeogenesis via SIRT2-p300-mediated PEPCK1 degradation and improves glucose homeostasis in diabetic mice. [Abstract]2021 Nov;42(11):1834-1846. PMID: 33574568 -
Neural Regen Res
Hippocampal insulin resistance and the Sirtuin 1 signaling pathway in diabetes-induced cognitive dysfunction. [Abstract]2021 Dec;16(12):2465-2474. PMID: 33907035 -
Life Sci
Nicotinamide mononucleotide attenuates isoproterenol-induced cardiac fibrosis by regulating oxidative stress and Smad3 acetylation. [Abstract]2021 Jun 1:274:119299. PMID: 33675899 -
J Cell Mol Med
UVA influenced the SIRT1-miR-27a-5p-SMAD2-MMP1/COL1/BCL2 axis in human skin primary fibroblasts. [Abstract]2020 Sep;24(17):10027-10041. PMID: 32790210 -
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Diabetol Metab Syndr
Electroacupuncture regulates Th17/Treg cell balance through SIRT1 mediated anti-inflammatory pathway to improve insulin resistance induced obesity. [Abstract]2025 Nov 14;17(1):427. PMID: 41239500 -
Mol Cell Biochem
Resveratrol induces apoptosis and inhibits adipogenesis by stimulating the SIRT1-AMPKα-FOXO1 signalling pathway in bovine intramuscular adipocytes. [Abstract]2018 Feb;439(1-2):213-223. PMID: 28819881
Sirtinol purchased from MedChemExpress. Usage Cited in: Mol Cell Biochem. 2018 Feb;439(1-2):213-223. [Abstract]
Western blot analysis in cells treated with 10 μM Sirtinol prior to incubation with 200 μM Resveratrol (RES) calculated from density values of specific protein bands/GAPDH density values and expressed as a percentage of the control.
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Cell Cycle
NaSH increases SIRT1 activity and autophagy flux through sulfhydration to protect SH-SY5Y cells induced by MPP~. [Abstract]2020 Sep;19(17):2216-2225. PMID: 32787548 -
Acta Histochem
Regulation of SIRT1-TLR2/TLR4 pathway in cell communication from macrophages to hepatic stellate cells contribute to alleviates hepatic fibrosis by Luteoloside. [Abstract]2022 Dec 16;125(1):151989. PMID: 36529079 -
Graefes Arch Clin Exp Ophthalmol
SIRT1 is required for the neuroprotection of resveratrol on retinal ganglion cells after retinal ischemia-reperfusion injury in mice. [Abstract]2020 Feb;258(2):335-344. PMID: 31900639 -
Acta Cardiol Sin
Sirt1 Inhibits Atrial Fibrosis by Downregulating the Expression of the Transforming Growth Factor-β1/Smad Pathway. [Abstract]2024 Mar;40(2):225-234. PMID: 38532813 -
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Lösungsmittel & Löslichkeit
DMSO : 11.67 mg/mL (29.58 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (2.54 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protokoll
Sirtinol is dissolved in 100% DMSO at concentration of 10 mM and stored at −20°C until use. The cell proliferation of H1299 cells is determined by trypan blue dye exclusion assay. Human nonsmall cell lung cancer (NSCLC) cells are seeded in 12-well plates and treated with indicated concentrations of sirtinol (0, 10, 20, and 50 μM) for 24 h and 48 h, respectively. After incubation, the cells are stained by 0.2% trypan blue and counted by Countess[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice: 30 male mice (20–25 g, 7–8 weeks old) are used in this model. Briefly, mice are randomly divided into five groups; then mice are intraperitoneally injected with 50 μL DMSO (vehicle group) or sirtinol (2.5 or 5.0 mg/kg body weight). After 1 h, paw inflammation is induced . The thickness of the paw is measured before and after HNE or saline injection[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Reinheit & Dokumentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Wang J, et al. Sirtinol, a class III HDAC inhibitor, induces apoptotic and autophagic cell death in MCF-7 human breast cancer cells. Int J Oncol. 2012 Sep;41(3):1101-1109. [Content Brief]
[2]. Fong Y, et al. The antiproliferative and apoptotic effects of sirtinol, a sirtuin inhibitor on human lung cancer cells by modulating Akt/β-catenin-Foxo3a axis. ScientificWorldJournal. 2014;2014:937051. [Content Brief]
[3]. Mai A, et al. Design, synthesis, and biological evaluation of sirtinol analogues as class III histone/protein deacetylase (Sirtuin) inhibitors. J Med Chem. 2005 Dec 1;48(24):7789-95. [Content Brief]
[4]. Tsai YF, et al. Sirtinol inhibits neutrophil elastase activity and attenuates lipopolysaccharide-mediated acute lung injury in mice. Sci Rep. 2015 Feb 10;5:8347. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.5350 mL | 12.6752 mL | 25.3505 mL | 63.3762 mL |
| 5 mM | 0.5070 mL | 2.5350 mL | 5.0701 mL | 12.6752 mL | |
| 10 mM | 0.2535 mL | 1.2675 mL | 2.5350 mL | 6.3376 mL | |
| 15 mM | 0.1690 mL | 0.8450 mL | 1.6900 mL | 4.2251 mL | |
| 20 mM | 0.1268 mL | 0.6338 mL | 1.2675 mL | 3.1688 mL | |
| 25 mM | 0.1014 mL | 0.5070 mL | 1.0140 mL | 2.5350 mL |
Sirtinol Related Classifications
HY-13515 Related Classifications
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