Art. -Nr.: HY-128359

ACBI1

11 Cited Publications

Kundenbewertung

Based on 11 publication(s) in Google Scholar

ACBI1 is a potent and cooperative SMARCA2, SMARCA4 and PBRM1 degrader with DC₅₀s of 6, 11 and 32 nM, respectively. ACBI1 is a PROTAC degrader. ACBI1 shows anti-proliferative activity. ACBI1 induces apoptosis.
(Pink: SMARCA2 and SMARCA4 and PBRM1 ligand (HY-44012); Blue: VHL ligand (HY-125905); Black: linker).

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The ACBI1 was designed by Boehringer Ingelheim and could be obtained free of charge through the Boehringer Ingelheim open innovation portal opnMe.com, associated with its negative control.

Reinheit: 99.92%
CAS. Nr.: 2375564-55-7
Formel: C₄₉H₅₈FN₉O₇S
Molecular Weight:936.10

Speicherung:

-20°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

ACBI1 Chemical Structure

CAS. Nr.: 2375564-55-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (11)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

ACBI1 is a potent and cooperative SMARCA2, SMARCA4 and PBRM1 degrader with DC₅₀s of 6, 11 and 32 nM, respectively. ACBI1 is a PROTAC degrader. ACBI1 shows anti-proliferative activity. ACBI1 induces apoptosis^[1]. (Pink: SMARCA2 and SMARCA4 and PBRM1 ligand (HY-44012); Blue: VHL ligand (HY-125905); Black: linker).

IC₅₀ & Target^[1]

VHL

SMARCA2

SMARCA4

PBRM1

Alle PROTACs Isoform-spezifische Produkte anzeigen :

Alle SWI/SNF Complex Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
MV4-11	IC₅₀	28 nM Compound: 25	Antiproliferative activity against human MV4-11 cells assessed as growth inhibition Antiproliferative activity against human MV4-11 cells assessed as growth inhibition	[PMID: 32858471]

In Vitro

ACBI1 (1-10000 nM； 3-7 days) shows anti-proliferative activity^[1].
ACBI1 (0.3 μM; 100 h) induces apoptosis in SK-MEL-5 cells^[1].
ACBI1 is composed of a bromodomain ligand, a linker, and the E3 ubiquitin ligase von Hippel-Lindau^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

MV-4-11, NCI-H1568 cells
Concentration:

0-1000 nM
Incubation Time:

3-7 days
Result:

Showed anti-proliferative activity with IC₅₀ of 29, 68 nM for MV-4-11, NCI-H1568 cells, respectively.

Apoptosis Analysis^[1]

Cell Line:

SK-MEL-5 cells
Concentration:

0.3 µM
Incubation Time:

100 h
Result:

Induced apoptosis in SK-MEL-5 cells.

Chemical Information

CAS. Nr. 2375564-55-7
Appearance Solid
Molecular Weight 936.10
Formel C₄₉H₅₈FN₉O₇S
Color White to yellow
SMILES

CC(N=CS1)=C1C2=CC=C(CNC([C@@H]3C[C@@H](O)CN3C([C@H](C(C)(C)C)NC(C4(CC4)F)=O)=O)=O)C(OCCOC5=CC=C(CN6CCN(C7=CC(C8=C(O)C=CC=C8)=NN=C7N)CC6)C=C5)=C2
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Publications (11)

All IFCell Proliferation/Viability AssayBio/Physico-chemical AssayWBCell Imaging/Staining

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Journal Impact Factor

Nat Genet

Pharmacological disruption of mSWI/SNF complex activity restricts SARS-CoV-2 infection. [Abstract]

2023 Mar;55(3):471-483. PMID: 36894709

ACBI1 purchased from MedChemExpress. Usage Cited in: Nat Genet. 2023 Mar;55(3):471-483. [Abstract]

ACBI1 (1.25, 2.5 μM; 2 days) inhibits ACE2 expression in both Vero E6 and Huh7.5 cells.
Nat Commun

PCGF1-PRC1 links chromatin repression with DNA replication during hematopoietic cell lineage commitment. [Abstract]

2022 Nov 28;13(1):7159. PMID: 36443290
Adv Sci (Weinh)

Arid1a Deficiency Drives Aristolochic Acid-Induced Liver Tumorigenesis through Ctnnb1 Mutation and Defective Nucleotide Excision Repair. [Abstract]

2025 Oct 24:e13981. PMID: 41133886
Nat Struct Mol Biol

Aberrant gene activation in synovial sarcoma relies on SSX specificity and increased PRC1.1 stability. [Abstract]

2023 Nov;30(11):1640-1652. PMID: 37735617

ACBI1 purchased from MedChemExpress. Usage Cited in: Nat Struct Mol Biol. 2023 Nov;30(11):1640-1652. [Abstract]

Immunofluorescence of MBD constructs (V5, cyan) and SS18-SSX1 (HA), SMARCA2 or SMARCC1 (magenta) in HS-SY-II cells not treated (MBD-Luc) or treated with DMSO or 500 nM ACBI1 (MBD-KDM2B).

ACBI1 purchased from MedChemExpress. Usage Cited in: Nat Struct Mol Biol. 2023 Nov;30(11):1640-1652. [Abstract]

Immunofluorescence of HEK293T cells expressing eGFP-SS18-SSX1 (cyan) treated with DMSO (top) or 500 nM ACBI1 (bottom) stained for BAF subunits (magenta) SMARCA2 (BRM, left), ARID1A (middle) or SMARCC1 (right). Images are representative of two biological replicates.

ACBI1 purchased from MedChemExpress. Usage Cited in: Nat Struct Mol Biol. 2023 Nov;30(11):1640-1652. [Abstract]

Western Blot of whole cell extracts from HS-SY-II or HEK293T cells collected 72 h without or with 500 nM ACBI1 treatment.

ACBI1 purchased from MedChemExpress. Usage Cited in: Nat Struct Mol Biol. 2023 Nov;30(11):1640-1652. [Abstract]

H3K27ac immunofluorescence of HEK293T cells expressing eGFP-EWSR1-SSX1 (left) or eGFP-MN1-SSX1 (right) treated with DMSO (top) or 500 nM ACBI1 (bottom) for SMARCA2 (BRM, left), ARID1A (middle) or SMARCC1 (right). Merge channels display eGFP (cyan) and BAF H3K27ac (magenta) overlays. Images are representative of three biological replicates.
Oncogenesis

The SWI/SNF ATPase BRG1 facilitates multiple pro-tumorigenic gene expression programs in SMARCB1-deficient cancer cells. [Abstract]

2022 Jun 1;11(1):30. PMID: 35650187

ACBI1 purchased from MedChemExpress. Usage Cited in: Oncogenesis. 2022 Jun 1;11(1):30. [Abstract]

G401 cells were treated with 250 nM ACBI1 for the indicated times and protein lysates were probed for expression of BRG1, pBAF-specific subunits PBRM1, ARID2, and BRD7, and MYC. DMSO control is matched to longest treatment. GAPDH is used as a loading control.

ACBI1 purchased from MedChemExpress. Usage Cited in: Oncogenesis. 2022 Jun 1;11(1):30. [Abstract]

G401 cells in c were allowed to grow for 4 days in the presence of ACBI1 or DMSO control and cells counted at this timepoint.

ACBI1 purchased from MedChemExpress. Usage Cited in: Oncogenesis. 2022 Jun 1;11(1):30. [Abstract]

Total number of differentially expressed genes that were increased in expression ("up") or decreased ("down") in response to 24 h ACBI1 treatment. FDR < 0.05 was used for determining significantly changed genes.
Structure

PROTAC-mediated activation, rather than degradation, of a nuclear receptor reveals complex ligand-receptor interaction network. [Abstract]

2024 Dec 5;32(12):2352-2363.e8. PMID: 39389062
Epigenetics Chromatin

Unraveling the cohesin-chromatin interface: identifying protein interactions that modulate chromosome structure and function. [Abstract]

2025 Jun 2;18(1):31. PMID: 40452075
bioRxiv

BAF complex-independent gene activation by SS18::SSX. [Abstract]

2026 Jan 27:2026.01.26.701739. PMID: 41659547
bioRxiv

Oncogenic virus hijacks SOX18 pioneer function to enhance viral persistence. [Abstract]

2025 Jul 17:2025.06.28.662102. PMID: 40631164
bioRxiv

2025 April 16.
bioRxiv

2025 March 03.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : ≥ 100 mg/mL (106.83 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.0683 mL

5.3413 mL

10.6826 mL

5 mM

0.2137 mL

1.0683 mL

2.1365 mL

10 mM

0.1068 mL

0.5341 mL

1.0683 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (2.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (2.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Farnaby W, et al. BAF complex vulnerabilities in cancer demonstrated via structure-based PROTAC design. Nat Chem Biol. 2019 Jul;15(7):672-680. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.0683 mL	5.3413 mL	10.6826 mL	26.7065 mL
	5 mM	0.2137 mL	1.0683 mL	2.1365 mL	5.3413 mL
	10 mM	0.1068 mL	0.5341 mL	1.0683 mL	2.6707 mL
	15 mM	0.0712 mL	0.3561 mL	0.7122 mL	1.7804 mL
	20 mM	0.0534 mL	0.2671 mL	0.5341 mL	1.3353 mL
	25 mM	0.0427 mL	0.2137 mL	0.4273 mL	1.0683 mL
	30 mM	0.0356 mL	0.1780 mL	0.3561 mL	0.8902 mL
	40 mM	0.0267 mL	0.1335 mL	0.2671 mL	0.6677 mL
	50 mM	0.0214 mL	0.1068 mL	0.2137 mL	0.5341 mL
	60 mM	0.0178 mL	0.0890 mL	0.1780 mL	0.4451 mL
	80 mM	0.0134 mL	0.0668 mL	0.1335 mL	0.3338 mL
	100 mM	0.0107 mL	0.0534 mL	0.1068 mL	0.2671 mL

Keywords

ACBI1

11 Cited Publications

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ACBI1

PROTAC Library

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) ACBI1

Alle PROTACs Isoform-spezifische Produkte anzeigen

Alle SWI/SNF Complex Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (11)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

ACBI1

PROTAC Library

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) ACBI1

Alle PROTACs Isoform-spezifische Produkte anzeigen

Alle SWI/SNF Complex Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (11)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

ACBI1 Related Classifications

HY-128359 Related Classifications