Art. -Nr.: HY-B2060

Tiamulin

(Synonyms: Thiamutilin)

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

Tiamulin (Thiamutilin) is a diterpenic antibiotic that is widely used in pigs and poultry for the control of infectious diseases. Tiamulin is effectively used in the study of airsacculitis, which is primarily caused by Mycoplasma spp.

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Reinheit: 99.74%
CAS. Nr.: 55297-95-5
Formel: C₂₈H₄₇NO₄S
Molecular Weight:493.74

Speicherung:

Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Tiamulin Chemical Structure

CAS. Nr.: 55297-95-5

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle Antibiotic Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	IC₅₀	>10 μM Compound: Tiamulin	Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 35220163]
HGC-27	IC₅₀	>10 μM Compound: Tiamulin	Cytotoxicity against human HGC-27 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against human HGC-27 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 35220163]
HT-29	IC₅₀	>10 μM Compound: Tiamulin	Cytotoxicity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 35220163]
MCF7	IC₅₀	>10 μM Compound: Tiamulin	Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 35220163]
U-87MG ATCC	IC₅₀	>10 μM Compound: Tiamulin	Cytotoxicity against human U87 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against human U87 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 35220163]

In Vitro

Tiamulin is a semisynthetic derivative of the diterpene antibiotic Pleuromutilin and is effectively used in the study of airsacculitis, which is primarily caused by Mycoplasma spp^[1].
Tiamulin is highly active in vitro against Mycoplasma strains (M. gallisepticum, M. synoviae, M. meleagridis, and M. iowae), Spirochaetes (Brachyspira hyodysenteriae, Brachyspira innocens, B. pilosicoli, B. intermedia), gram-positive bacteria (staphylococci, streptococci, Clostridia, Arcanobacterium spp), but less active against gram-negative bacteria (Pasteurella, Klebsiella, Haemophilus, Fusobacterium, Campylobacter, Bacteroides spp.)^[1].
Tiamulin binds with the rRNA in the peptidyl transferase slot on the ribosome, in which it prevents the correct positioning of the CCA ends of tRNA for peptide transferase and subsequent protein production^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Tiamulin is highly effective in the treatment of avian intestinal spirochaetosis in breeder and layer hens at 25 mg/kg of BW per day over 5 d in artificial infection studies with B. pilosicoli and B. intermedia, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 55297-95-5
Appearance Oil
Molecular Weight 493.74
Formel C₂₈H₄₇NO₄S
Color Light yellow to brown
SMILES

O=C(O[C@H]1[C@@]([C@H](C)CC2)(C)[C@@](C(CC3)=O)([H])[C@]32[C@@H](C)[C@H](O)[C@](C)(C=C)C1)CSCCN(CC)CC
Synonyms

Thiamutilin
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Pathogens

Mycoplasma bovis Invades Non-Phagocytic Cells by Clathrin-Dependent Endocytic Pathways and Escapes from Phagocytic Vesicles. [Abstract]

2024 Nov 15;13(11):1003. PMID: 39599557

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (202.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0254 mL

10.1268 mL

20.2536 mL

5 mM

0.4051 mL

2.0254 mL

4.0507 mL

10 mM

0.2025 mL

1.0127 mL

2.0254 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 5 mg/mL (10.13 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (10.13 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Islam KM, et al. The activity and compatibility of the antibiotic tiamulin with other drugs in poultry medicine--A review. Poult Sci. 2009 Nov;88(11):2353-9. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.0254 mL	10.1268 mL	20.2536 mL	50.6339 mL
	5 mM	0.4051 mL	2.0254 mL	4.0507 mL	10.1268 mL
	10 mM	0.2025 mL	1.0127 mL	2.0254 mL	5.0634 mL
	15 mM	0.1350 mL	0.6751 mL	1.3502 mL	3.3756 mL
	20 mM	0.1013 mL	0.5063 mL	1.0127 mL	2.5317 mL
	25 mM	0.0810 mL	0.4051 mL	0.8101 mL	2.0254 mL
	30 mM	0.0675 mL	0.3376 mL	0.6751 mL	1.6878 mL
	40 mM	0.0506 mL	0.2532 mL	0.5063 mL	1.2658 mL
	50 mM	0.0405 mL	0.2025 mL	0.4051 mL	1.0127 mL
	60 mM	0.0338 mL	0.1688 mL	0.3376 mL	0.8439 mL
	80 mM	0.0253 mL	0.1266 mL	0.2532 mL	0.6329 mL
	100 mM	0.0203 mL	0.1013 mL	0.2025 mL	0.5063 mL

Keywords

Tiamulin

1 Cited Publications

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Tiamulin

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Publications Citing Use of MedChemExpress (MCE) Tiamulin

Alle Antibiotic Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Tiamulin

Anti-Infection Compound Library

Antibacterial Compound Library

FDA Approved & Pharmacopeial Drug Library

Antibiotics Library

Cysteine Targeted Covalent Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product-like Compound Library

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) Tiamulin

Alle Antibiotic Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Tiamulin Related Classifications

HY-B2060 Related Classifications