Cat. No.: HY-N0155

Nobiletin

27 Cited Publications

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Based on 27 publication(s) in Google Scholar

Nobiletin is a poly-methoxylated flavone from the citrus peel that improves memory loss. Nobiletin is a retinoid acid receptor-related orphan receptors (RORs) agonist. Nobiletin can reduce reactive oxygen species (ROS) levels in differentiated C2C12 myotubes and has anti-inflammation and anti-cancer properties, including anti-angiogenesis, anti-proliferation, anti-metastasis and induced apoptosis.

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Pureté: 99.79%
CAS No.: 478-01-3
Formule: C₂₁H₂₂O₈
Masse moléculaire:402.39

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

Nobiletin Chemical Structure

CAS No.: 478-01-3

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Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (27)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Bioz

Activité biologique

Description

IC₅₀ & Target

Retinoid acid receptor-related orphan receptors (RORs)^[1]; reactive oxygen species (ROS)^[1]; apoptosis^[2]

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Cellular Effect

Cell Line	Type	Value	Description	References
3T3-L1	IC₅₀	50 μM Compound: 4, Nobiletin	Anticorpulence activity against mouse 3T3L1 cells assessed as inhibition of lipid droplet accumulation Anticorpulence activity against mouse 3T3L1 cells assessed as inhibition of lipid droplet accumulation	[PMID: 19054677]
A2780/Taxol	IC₅₀	9 μM Compound: 48	Antiproliferative activity against human A2780/Taxol cells overexpressing ABCB1 assessed as cell growth inhibition by MTT assay Antiproliferative activity against human A2780/Taxol cells overexpressing ABCB1 assessed as cell growth inhibition by MTT assay	[PMID: 35751979]
A549/TR	IC₅₀	9 μM Compound: 48	Antiproliferative activity against human A549/Taxol cells overexpressing ABCB1 assessed as cell growth inhibition by MTT assay Antiproliferative activity against human A549/Taxol cells overexpressing ABCB1 assessed as cell growth inhibition by MTT assay	[PMID: 35751979]
CWR22R	IC₅₀	36.53 μM Compound: 44	Antiproliferative activity against human 22Rv1 cells incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human 22Rv1 cells incubated for 24 hrs by CCK-8 assay	[PMID: 36459083]
DU-145	IC₅₀	144.5 μM Compound: 44	Antiproliferative activity against human DU-145 cells incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human DU-145 cells incubated for 24 hrs by CCK-8 assay	[PMID: 36459083]
HL-60	IC₅₀	10 ng/mL Compound: Nobiletin	Growth inhibition of human HL60 cells after 72 hrs by MTT assay Growth inhibition of human HL60 cells after 72 hrs by MTT assay	[PMID: 31398616]
HL-60	IC₅₀	41.5 μM Compound: I	Antiproliferative activity against HL60 after 24 hrs Antiproliferative activity against HL60 after 24 hrs	[PMID: 17391969]
HT-29	IC₅₀	24 μM Compound: 4, Nobiletin	Anticancer activity against human HT-29 cells after 72 hrs by MTT assay Anticancer activity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 19054677]
KB	ED₅₀	3 μg/mL Compound: NSC-76751	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 469554]
LNCaP C4-2	IC₅₀	30.3 μM Compound: 44	Antiproliferative activity against human C4-2 cells incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human C4-2 cells incubated for 24 hrs by CCK-8 assay	[PMID: 36459083]
MCF7	IC₅₀	4.4 μM Compound: 20	Inhibition of BCRP expressed in MCF-7 MX cells using Hoechst 33342 staining Inhibition of BCRP expressed in MCF-7 MX cells using Hoechst 33342 staining	[PMID: 21354800]
MDCK	IC₅₀	4.9 μM Compound: 20	Inhibition of BCRP expressed in MDCK cells using Hoechst 33342 staining Inhibition of BCRP expressed in MDCK cells using Hoechst 33342 staining	[PMID: 21354800]
Monocyte	IC₅₀	10 μM Compound: Nobiletin	Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA	[PMID: 10096854]
NIH-3T3-G185	IC₅₀	11.5 μM Compound: Nobiletin	TP_TRANSPORTER: inhibition of Daunorubicin efflux in NIH-3T3-G185 cells TP_TRANSPORTER: inhibition of Daunorubicin efflux in NIH-3T3-G185 cells	[PMID: 11743742]
PC-3	IC₅₀	122.6 μM Compound: 44	Antiproliferative activity against human PC-3 cells incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human PC-3 cells incubated for 24 hrs by CCK-8 assay	[PMID: 36459083]

In Vitro

Nobiletin (0-100 μM; 24 hours; U2OS and HOS cells) treatment progressively reduces protein expressions of MMP-2 and MMP-9. In U2OS and HOS cells, Nobiletin considerably reduces the phosphorylation of p-IKKα/β and p-IκBα, and protein expression of NF-κB in the cell nuclear fraction with the concomitant increase of the NF-κB expression in the cytosolic fraction. Nobiletin down-regulates the p-CREB and the SP-1 expressions in the nuclear fraction, whereas Nobiletin does not affect c-Jun and c-Fos expressions^[1].
Nobiletin (0-100 μM; 24 hours; U2OS and HOS cells) treatment significantly reduces mRNA expressions of MMP-2 and MMP-9 dose-dependently in U2OS and HOS cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

U2OS and HOS cells
Concentration:

0 μM, 25 μM, 50 μM, 75 μM, 100 μM
Incubation Time:

24 hours
Result:

Progressively reduced protein expressions of MMP-2 and MMP-9. Considerably reduced the phosphorylation of p-IKKα/β and p-IκBα, and protein expression of NF-κB in the cell nuclear fraction with the concomitant increase of the NF-κB expression in the cytosolic fraction. Down-regulated the p-CREB and the SP-1 expressions in the nuclear fraction in U2OS and HOS cells.

RT-PCR^[1]

Cell Line:

U2OS and HOS cells
Concentration:

0 μM, 25 μM, 50 μM, 75 μM, 100 μM
Incubation Time:

24 hours
Result:

Significantly reduced mRNA expressions of MMP-2 and MMP-9 dose-dependently in U2OS and HOS cells.

In Vivo

Nobiletin (0.1% of regular diet; oral administration; daily; for 20 weeks; male C57BL/6 mice) treatment restores glucose homeostasis and promots energy expenditure and circadian activity in aged mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

20- to 22-month-old male C57BL/6 mice^[2]
Dosage:

0.1% of regular diet
Administration:

Oral administration; daily; for 20 weeks
Result:

Fully restored glucose tolerance in aged mice, and increased basal body temperature and cold tolerance in aged mice. Led to a twofold increase in distance run/day on voluntary wheels compared with aged regular diet-fed mice during the active phase.

Chemical Information

CAS No. 478-01-3
Appearance Solid
Masse moléculaire 402.39
Formule C₂₁H₂₂O₈
Color White to light yellow
SMILES

O=C1C=C(C2=CC=C(OC)C(OC)=C2)OC3=C(OC)C(OC)=C(OC)C(OC)=C13
Structure Classification
- Flavonoids
- Flavones
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (27)

All Cell Proliferation/Viability AssayFlow CytometryBio/Physico-chemical AssayWB

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Journal Impact Factor

Nat Aging

Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice. [Abstract]

2024 Sep;4(9):1231-1248. PMID: 38951692
Acta Pharm Sin B

Chrysin serves as a novel inhibitor of DGK α/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). [Abstract]

2021 Jan;11(1):143-155. PMID: 33532186
Int J Biol Sci

Nobiletin suppresses cholangiocarcinoma proliferation via inhibiting GSK3β. [Abstract]

2022 Sep 11;18(15):5698-5712. PMID: 36263164

Nobiletin purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Sep 11;18(15):5698-5712. [Abstract]

The viability of primary hepatocyte was assessed after treatment with different doses of NBT (Nobiletin, 0-200 μM) for 48h.

Nobiletin purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Sep 11;18(15):5698-5712. [Abstract]

The viability of TFK1 cells and RBE cells was assessed after treatment with different doses of NBT (Nobiletin, 0-100 μM) and at different times (0-48 h).

Nobiletin purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Sep 11;18(15):5698-5712. [Abstract]

Representative results of cell cycle and quantitative analyses after 48 hours of NBT (Nobiletin, 0-100 μM) treatment.

Nobiletin purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Sep 11;18(15):5698-5712. [Abstract]

The intersection of target proteins of NBT (Nobiletin) and CCA.

Nobiletin purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Sep 11;18(15):5698-5712. [Abstract]

The expression of G0/G1 cell cycle signal regulators, Cyclin D1 and CDK4 were examined by western blot after NBT (Nobiletin, 0-100 μM) treatment for 24 hours and 48 hours.
Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530
Ind Crops Prod

2025 Dec 11;239:122458.
J Agric Food Chem

Tangeretin Inhibits BACE1 Activity and Attenuates Cognitive Impairments in AD Model Mice. [Abstract]

2022 Feb 9;70(5):1536-1546. PMID: 35084179
J Ethnopharmacol

Nobiletin from Citrus reticulata Blanco alleviates pulmonary fibrosis through inhibiting the PI3K/AKT pathway and epithelial-mesenchymal transition. [Abstract]

2025 May 10:119965. PMID: 40354836
Food Funct

Nobiletin protects against ferroptosis to alleviate sepsis-associated acute liver injury by modulating the gut microbiota. [Abstract]

2023 Aug 14;14(16):7692-7704. PMID: 37545398
Nutrients

Nobiletin Mitigates D-Galactose-Induced Memory Impairment via Improving Hippocampal Neurogenesis in Mice. [Abstract]

2023 May 8;15(9):2228. PMID: 37432372
Nutrients

Nobiletin Improves D-Galactose-Induced Aging Mice Skeletal Muscle Atrophy by Regulating Protein Homeostasis. [Abstract]

2023 Apr 7;15(8):1801. PMID: 37111020

Nobiletin purchased from MedChemExpress. Usage Cited in: Nutrients. 2023 Apr 7;15(8):1801. [Abstract]

Nobiletin (Nob; 100 mg/kg/day; s.c.; 10 weeks) markedly reduces D-gal (D-galactose)-induced increases in the ratio of p-FOXO 3a/FOXO and the expression of MAFbx and MuRF1 in mice.
Eur J Pharmacol

Nobiletin ameliorates intervertebral disc degeneration by upregulating HIF-1α to inhibit endoplasmic reticulum stress-induced apoptosis. [Abstract]

2026 Jan 12:1011:178480. PMID: 41397628
Lipids Health Dis

Nobiletin alleviates atherosclerosis by inhibiting lipid uptake via the PPARG/CD36 pathway. [Abstract]

2024 Mar 11;23(1):76. PMID: 38468335
Sci Rep

Nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation. [Abstract]

2021 Jun 3;11(1):11796. PMID: 34083641
Food Sci Nutr

Nobiletin Ameliorates Skeletal Muscle Performance in D-Galactose-Induced Aging Mice by Boosting Aerobic Metabolism. [Abstract]

2026 Jan 9;14(1):e71416. PMID: 41523277
J Pharm Pharmacol

Nobiletin attenuates monocrotaline-induced pulmonary arterial hypertension through PI3K/Akt/STAT3 pathway. [Abstract]

2023 Aug 1;75(8):1100-1110. PMID: 37158759
Biol Reprod

Nobiletin-induced partial abrogation of deleterious effects of AKT inhibition on preimplantation bovine embryo development in vitro†. [Abstract]

2021 Dec 20;105(6):1427-1442. PMID: 34617564
J Nat Med

Nobiletin alleviates hypoxia-induced pulmonary hypertension by inhibiting calcium-sensing receptor. [Abstract]

2025 Sep;79(5):1154-1166. PMID: 40581895
Theriogenology

Nobiletin as a novel agent to enhance porcine in vitro embryo development and quality. [Abstract]

2024 Jul 15:223:36-46. PMID: 38669840
Biochem Biophys Res Commun

Nobiletin enhances mitochondrial function by regulating SIRT1/PGC-1α signaling in porcine oocytes during in vitro maturation. [Abstract]

2024 Apr 30:706:149747. PMID: 38479243
Kidney Blood Press Res

Nobiletin from Xiaoyu Xiezhuo Decoction: Restoring Mitochondrial Dynamics and Alleviating Renal Ischemia-Reperfusion Injury via M1 Macrophage Modulation. [Abstract]

2026 Mar 17:1-18. PMID: 41843713
Reprod Fert Develop

2021, 33(2): 135-135.
Reproduction, Fertility and Development

2019, 32, 164-165.
Reprod Fert Develop

2018, 31(1): 212-212.
SSRN

2025 Dec 31.
Research Square Print

2023 Feb 1.
E3S Web of Conferences

233, 02010 (2021).

Solvant et solubilité

In Vitro:

DMSO : 50 mg/mL (124.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4852 mL

12.4258 mL

24.8515 mL

5 mM

0.4970 mL

2.4852 mL

4.9703 mL

10 mM

0.2485 mL

1.2426 mL

2.4852 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (5.17 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (5.17 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Cheng HL, et al. Nobiletin inhibits human osteosarcoma cells metastasis by blocking ERK and JNK-mediated MMPs expression. Oncotarget. 2016 Jun 7;7(23):35208-23. [Content Brief]

[2]. Nohara K, et al. Nobiletin fortifies mitochondrial respiration in skeletal muscle to promote healthy aging against metabolic challenge. Nat Commun. 2019 Aug 28;10(1):3923. [Content Brief]

[3]. Takito J, et al. Nerve growth factor enhances the CRE-dependent transcriptional activity activated by nobiletin in PC12 cells. Can J Physiol Pharmacol. 2016 Jul;94(7):728-33. [Content Brief]

[4]. He B, et al. The Small Molecule Nobiletin Targets the Molecular Oscillator to Enhance Circadian Rhythms and Protect against Metabolic Syndrome. Cell Metab. 2016 Apr 12;23(4):610-21. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4852 mL	12.4258 mL	24.8515 mL	62.1288 mL
	5 mM	0.4970 mL	2.4852 mL	4.9703 mL	12.4258 mL
	10 mM	0.2485 mL	1.2426 mL	2.4852 mL	6.2129 mL
	15 mM	0.1657 mL	0.8284 mL	1.6568 mL	4.1419 mL
	20 mM	0.1243 mL	0.6213 mL	1.2426 mL	3.1064 mL
	25 mM	0.0994 mL	0.4970 mL	0.9941 mL	2.4852 mL
	30 mM	0.0828 mL	0.4142 mL	0.8284 mL	2.0710 mL
	40 mM	0.0621 mL	0.3106 mL	0.6213 mL	1.5532 mL
	50 mM	0.0497 mL	0.2485 mL	0.4970 mL	1.2426 mL
	60 mM	0.0414 mL	0.2071 mL	0.4142 mL	1.0355 mL
	80 mM	0.0311 mL	0.1553 mL	0.3106 mL	0.7766 mL
	100 mM	0.0249 mL	0.1243 mL	0.2485 mL	0.6213 mL

Keywords

Nobiletin

27 Cited Publications

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Nobiletin

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Publications Citing Use of MedChemExpress (MCE) Nobiletin

Voir tous les produits spécifiques à Isoform ROR

Activité biologique

Chemical Information

Publications (27)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Nobiletin Related Classifications

Cat. No.: HY-N0155