1. Cell Cycle/DNA Damage
    Metabolic Enzyme/Protease
  2. HSP
  3. Col003

Col003 

Cat. No.: HY-124817 Purity: 99.30%
Handling Instructions

Col003 is a selective and potent inhibitor of Hsp47 and competitively binds to the collagen binding site on Hsp47 (IC50=1.8 μM). Col003 discourages the interaction of Hsp47 with collagen and inhibits collagen secretion by destabilizing the collagen triple helix. Col003 can be used for the investigation of fibrosis

For research use only. We do not sell to patients.

Col003 Chemical Structure

Col003 Chemical Structure

CAS No. : 328565-16-8

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Based on 1 publication(s) in Google Scholar

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Description

Col003 is a selective and potent inhibitor of Hsp47 and competitively binds to the collagen binding site on Hsp47 (IC50=1.8 μM). Col003 discourages the interaction of Hsp47 with collagen and inhibits collagen secretion by destabilizing the collagen triple helix. Col003 can be used for the investigation of fibrosis[1]

IC50 & Target[1]

HSP47

1.8 μM (IC50)

In Vitro

Col003 (4.4-40 μM) is cleaved from Col001 (AK-778). Col003 has an inhibitory effect on the interaction of Hsp47 with collagen in Hsp47 KO −/− MEFs[1].
Col003 (0.01-100 μM) exhibits dose-dependent effects on the interactions of Hsp47 with collagen. The IC50 value is 1.8 μM for Hsp47[1].
Col003 (100 μM; 20-60 mins) has an nhibitory effects on collagen secretion and accumulation. It inhibits collagen secretion by wild-type MEFs and the secretion is not abolished completely[1].
Col003 (100 μM) degrades α,α′-dipyridyl collagen secretion completely by incubation with trypsin at a high temperature (50 °C for 15 min), but it resistant to trypsin digestion at 37 °C[1].
 .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: Wild-type MEFs
Concentration: 100 μM
Incubation Time: After 50 °C or 37 °C for 15 min
Result: Affected correctly folded triple helix structures and α,α′-dipyridyl on collagen secretion.
Molecular Weight

257.24

Formula

C₁₄H₁₁NO₄

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (194.37 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8874 mL 19.4371 mL 38.8742 mL
5 mM 0.7775 mL 3.8874 mL 7.7748 mL
10 mM 0.3887 mL 1.9437 mL 3.8874 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.5 mg/mL (9.72 mM); Clear solution; Need ultrasonic

*All of the co-solvents are provided by MCE.
References

Purity: 99.30%

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Col003
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