COX-2-IN-66
COX-2-IN-66 is a cyclooxygenase-2 (COX-2) inhibitor with an IC50 of 8.43 μM. COX-2-IN-66 reduces release of pro-inflammatory cytokines IL-6 and TNF-α. COX-2-IN-66 exhibits acceptable cellular tolerability, with cytokine-modulating concentrations remaining below its cytotoxic threshold. COX-2-IN-66 can be used for the research of inflammation.
For research use only. We do not sell to patients.
- Formula: C23H23NO2
- Molecular Weight:345.43
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
|
COX-2 8.43 μM (IC50) |
COX-1 32.47 μM (IC50) |
IL-6 |
COX-2-IN-66 (Compound 35e) potently inhibits cell-free COX-2 enzymatic activity with an IC50 of 8.43 μM[1].
COX-2-IN-66 inhibits cell-free COX-1 enzymatic activity with an IC50 of 32.47 μM, yielding a COX-1/COX-2 selectivity index of 3.85[1].
COX-2-IN-66 (3.125-100 μM; 24 h) exhibits cytotoxicity in THP-1 macrophages with an IC50 of 67.9 μM[1].
COX-2-IN-66 (2.1-33.7 μM; 22 h) reduces IL-6 and TNF-α release in LPS (HY-D1056)-stimulated THP-1 macrophages with IC50 values of 35.1 μM and 42.3 μM, respectively, under non-cytotoxic conditions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:THP-1 macrophages
-
Concentration:3.125, 6.25, 25, 50, 100 μM
-
Incubation Time:24 h
-
Result:Showed a cytotoxic IC50 of 67.9 μM in THP-1 macrophages, which was higher than the concentrations required for cytokine modulation.
-
Cell Line:LPS-stimulated THP-1 macrophages
-
Concentration:2.1, 4.2, 8.4, 16.8, 33.7 μM; 35.1 μM
-
Incubation Time:22 h
-
Result:Reduced IL-6 and TNF-α release in a concentration-dependent manner, with estimated IC50 values of 35.1 μM for IL-6 and 42.3 μM for TNF-α.
These concentrations were below the cytotoxic IC50 in THP-1 cells.
Chemical Information
-
Molecular Weight 345.43
-
Formula C23H23NO2
-
SMILES
COC1=CC=C(C(C2=CC=C(C)C=C2)N3CCC4=C3C=CC=C4)C(O)=C1
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz