COX-2-IN-66 

COX-2-IN-66 is a cyclooxygenase-2 (COX-2) inhibitor with an IC₅₀ of 8.43 μM. COX-2-IN-66 reduces release of pro-inflammatory cytokines IL-6 and TNF-α. COX-2-IN-66 exhibits acceptable cellular tolerability, with cytokine-modulating concentrations remaining below its cytotoxic threshold. COX-2-IN-66 can be used for the research of inflammation^[1].

COX-2-IN-66 (Compound 35e) potently inhibits cell-free COX-2 enzymatic activity with an IC₅₀ of 8.43 μM^[1].
COX-2-IN-66 inhibits cell-free COX-1 enzymatic activity with an IC₅₀ of 32.47 μM, yielding a COX-1/COX-2 selectivity index of 3.85^[1].
COX-2-IN-66 (3.125-100 μM; 24 h) exhibits cytotoxicity in THP-1 macrophages with an IC₅₀ of 67.9 μM^[1].
COX-2-IN-66 (2.1-33.7 μM; 22 h) reduces IL-6 and TNF-α release in LPS (HY-D1056)-stimulated THP-1 macrophages with IC₅₀ values of 35.1 μM and 42.3 μM, respectively, under non-cytotoxic conditions^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

345.43

C₂₃H₂₃NO₂

COC1=CC=C(C(C2=CC=C(C)C=C2)N3CCC4=C3C=CC=C4)C(O)=C1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Pacheco JA, et al. Binding pose analysis and scaffold optimization of petasis products for selective COX-2 inhibition. Eur J Med Chem. Published online April 20, 2026.