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Cat. No.: HY-N21802

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Dendrocandin U

Dendrocandin U is a bibenzyl compound found in Dendrobium officinale that exhibits anti-inflammatory activity and α-glucosidase inhibitory effect (IC₅₀ = 9.46 mM). Dendrocandin U inhibits the expression of TLR4 and MyD88 in the TLR4/MyD88/NF-kB pathway, suppresses the phosphorylation of NF-kB p65 and I-kBα, and blocks the nuclear translocation of NF-kB p65. Dendrocandin U inhibits M1 polarization, NO secretion, and TNF-α release in alveolar macrophages, and reduces inflammatory morphological changes in macrophages. Dendrocandin U promotes neurite outgrowth in PC12 cells. Dendrocandin U can be used for research on inflammation-related diseases and type 2 diabetes.

For research use only. We do not sell to patients.

CAS No.: 1922084-93-2
Formula: C₂₆H₂₈O₈
Molecular Weight:468.50

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Dendrocandin U Chemical Structure

CAS No.: 1922084-93-2

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[1]^[2]

NF-κB

TLR4

iNOS

α‑glucosidase

TNF-α

All Toll-like Receptor (TLR) Isoforms :

All NF-κB Isoforms :

All NO Synthase Isoforms :

All TNF Receptor Isoforms :

In Vitro

Dendrocandin U (compound 1) (0.01-80 μM; 24 h) shows no cytotoxicity against MH-S cells at concentrations below 20 μM^[1].
Dendrocandin U (0.01-20 μM; 2 h pre-incubation) inhibits NO secretion in LPS/IFN-γ-stimulated MH-S cells^[1].
Dendrocandin U (5-20 μM; 2 h pre-incubation) inhibits TNF-α release in LPS/IFN-γ-stimulated MH-S cells at 10 μM^[1].
Dendrocandin U (20 μM; 2 h pre-incubation) attenuates M1-like morphological changes in LPS/IFN-γ-stimulated MH-S cells; inhibits the TLR4/MyD88/NF-κB signaling pathway and nuclear translocation of p-NF-κB p65 in stimulated MH-S cells^[1].
Dendrocandin U (10-20 μM; 2 h pre-incubation) inhibits the expression of M1 markers CD86 and iNOS in stimulated MH-S cells^[1].
Dendrocandin U (compound 11) is a weak α-glucosidase inhibitor with an IC₅₀ of 9.46 mM^[2].
Dendrocandin U (compound 2) (10 μM; 72 h) shows weak neurite outgrowth-promoting activity in PC12 cells, with a differentiation rate of 9.46%^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

MH-S alveolar macrophages
Concentration:

0.01, 0.1, 1, 5, 10, 20, 40, 80 μM
Incubation Time:

24 h
Result:

Did not exhibit obvious cytotoxicity at doses below 20 μM.

ELISA Assay^[1]

Cell Line:

MH-S alveolar macrophages
Concentration:

5, 10, 20 μM
Incubation Time:

2 h (pre-incubation); 24 h (stimulated with LPS (1 μg/mL) + IFN-γ (20 ng/mL))
Result:

Inhibited the release of TNF-α at 10 μM.

Immunofluorescence^[1]

Cell Line:

MH-S alveolar macrophages
Concentration:

0, 20 μM
Incubation Time:

2 h (pre-incubation); 6 h (stimulated with LPS (1 μg/mL) + IFN-γ (20 ng/mL))
Result:

Decreased the number of pseudopodia and spines on the cell surface, indicating it alleviates the inflammatory morphological changes induced by LPS and IFN-γ.

Western Blot Analysis^[1]

Cell Line:

MH-S alveolar macrophages
Concentration:

0, 10, 20 μM
Incubation Time:

2 h (pre-treatment); 24 h (stimulated with LPS (1 μg/mL) + IFN-γ (20 ng/mL))
Result:

Inhibited the expression of iNOS at 10 μM and 20 μM.

Western Blot Analysis^[1]

Cell Line:

MH-S alveolar macrophages
Concentration:

0, 20 μM
Incubation Time:

2 h (pre-treatment); 24 h (stimulated with LPS (1 μg/mL) + IFN-γ (20 ng/mL))
Result:

Significantly inhibited the expression of TLR4 (P <0.05), MyD88 (P<0.01), p-I-κBα (P<0.05), and p-NF-κB p65 (P < 0.01) at 20 μM.\nDecreased the nuclear p-NF-κB p65 (Ser536) protein level (P < 0.05), while the expression of p-NF-κB p65 (Ser536) in the cytoplasm was not affected (P > 0.05).

Immunofluorescence^[1]

Cell Line:

MH-S alveolar macrophages
Concentration:

0, 20 μM
Incubation Time:

2 h (pre-incubation); 2 h (stimulated with LPS (1 μg/mL) + IFN-γ (20 ng/mL))
Result:

Decreased the fluorescence intensity of nuclear p-NF-κB p65 (Ser536).

Chemical Information

CAS No. 1922084-93-2
Molecular Weight 468.50
Formula C₂₆H₂₈O₈
SMILES

C(O)[C@H]1[C@@H](OC=2C(O1)=C(OC)C=C(CCC3=CC=C(O)C=C3)C2)C4=CC(OC)=C(O)C(OC)=C4
Structure Classification
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Piao X M, et al. Dendrocandin U from Dendrobium officinale Kimura et Migo Inhibits M1 Polarization in Alveolar Macrophage by Suppressing NF‑κB Signaling Pathway. Chemistry & Biodiversity, 2023, 20(11): e202300999.

[2]. Chu C, et al. Antidiabetic constituents of Dendrobium officinale as determined by high-resolution profiling of radical scavenging and α-glucosidase and α-amylase inhibition combined with HPLC-PDA-HRMS-SPE-NMR analysis. Phytochemistry Letters, 2019, 31: 47-52.

[3]. Yang L, et al. Two new dendrocandins with neurite outgrowth-promoting activity from Dendrobium officinale. Journal of Asian natural products research. 2015;17(2):125-31.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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Dendrocandin U

All Toll-like Receptor (TLR) Isoforms

All NF-κB Isoforms

All NO Synthase Isoforms

All TNF Receptor Isoforms