Cat. No.: HY-129461

Dibenzyl trisulfide

(Synonyms: DTS)

Customer Review

Based on 1 Customer Validation

Dibenzyl trisulfide (DTS) is an active ingredient that can be isolated from Petiveria alliacea L.. Dibenzyl trisulfide inhibits cell proliferation and migration. Dibenzyl trisulfide decreased the mRNA and protein expression of BAK-1 and LTA. Dibenzyl trisulfide induces lysosomal membrane permeabilization and cathepsin B release.

For research use only. We do not sell to patients.

Purity: 98.24%
CAS No.: 6493-73-8
Formula: C₁₄H₁₄S₃
Molecular Weight:278.46

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

Dibenzyl trisulfide Chemical Structure

CAS No.: 6493-73-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- Clinical Trial
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All Cathepsin Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	0.4 μM Compound: 4	Antitumor activity against human A2780 cell line by RTCES assay Antitumor activity against human A2780 cell line by RTCES assay	[PMID: 16828553]
HeLa	IC₅₀	2.5 μM Compound: 4	Antitumor activity against human HeLa cell line by RTCES assay Antitumor activity against human HeLa cell line by RTCES assay	[PMID: 16828553]
HepG2	IC₅₀	>100 μM Compound: 4	Antitumor activity against human HepG2 cell line by RTCES assay Antitumor activity against human HepG2 cell line by RTCES assay	[PMID: 16828553]
HT-1080	IC₅₀	1.9 μM Compound: 4	Antitumor activity against human HT1080 cell line by RTCES assay Antitumor activity against human HT1080 cell line by RTCES assay	[PMID: 16828553]
Jurkat	IC₅₀	0.35 μM Compound: 4	Antitumor activity against human jurkat cell line by MTT assay Antitumor activity against human jurkat cell line by MTT assay	[PMID: 16828553]
MCF7	IC₅₀	6.6 μM Compound: 4	Antitumor activity against human MCF7 cell line by RTCES assay Antitumor activity against human MCF7 cell line by RTCES assay	[PMID: 16828553]
MDA-MB-231	IC₅₀	2.4 μM Compound: 4	Antitumor activity against human M231 cell line by RTCES assay Antitumor activity against human M231 cell line by RTCES assay	[PMID: 16828553]
NCI-H460	IC₅₀	5.1 μM Compound: 4	Antitumor activity against human H460 cell line by RTCES assay Antitumor activity against human H460 cell line by RTCES assay	[PMID: 16828553]
OVCAR-4	IC₅₀	1.4 μM Compound: 4	Antitumor activity against human OVACR4 cell line by RTCES assay Antitumor activity against human OVACR4 cell line by RTCES assay	[PMID: 16828553]

In Vitro

Dibenzyl trisulfide (0.1-50 µM; 2 weeks) inhibits CRL-2335 cell proliferation and migration^[1].
Dibenzyl trisulfide (10, 50 µM; 48 h) decreased the mRNA and protein expression of BAK-1 and LTA in a dose-dependent manner^[1].
Dibenzyl trisulfide (10 µM; 48 h) induces lysosomal membrane permeabilization and cathepsin B release in CRL-2335 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

CRL-2335 cells
Concentration:

0.1, 0.5, 1, 10, 50 µM
Incubation Time:

2 weeks
Result:

Inhibited proliferation and migration of CRL-2335 cell in a dose-dependent manner.

RT-PCR^[1]

Cell Line:

CRL-2335 cells
Concentration:

0, 10, 50 µM
Incubation Time:

48 h
Result:

Decreased the mRNA expression of BAK-1, GADD45a, and LTA in a dose-dependent manner.

Immunofluorescence^[1]

Cell Line:

CRL-2335 cells
Concentration:

0, 10, 50 µM
Incubation Time:

48 h
Result:

Decreased the protein expression of BAK-1 and LTA but not GADD45a.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 6493-73-8
Appearance Solid
Molecular Weight 278.46
Formula C₁₄H₁₄S₃
Color White to off-white
SMILES

C1(CSSSCC2=CC=CC=C2)=CC=CC=C1
Synonyms

DTS
Structure Classification
- Others
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (359.12 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.5912 mL

17.9559 mL

35.9118 mL

5 mM

0.7182 mL

3.5912 mL

7.1824 mL

10 mM

0.3591 mL

1.7956 mL

3.5912 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Williams LA, et al. Implications of dibenzyl trisulphide for disease treatment based on its mode of action. West Indian Med J. 2009 Nov;58(5):407-9. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.5912 mL	17.9559 mL	35.9118 mL	89.7795 mL
	5 mM	0.7182 mL	3.5912 mL	7.1824 mL	17.9559 mL
	10 mM	0.3591 mL	1.7956 mL	3.5912 mL	8.9780 mL
	15 mM	0.2394 mL	1.1971 mL	2.3941 mL	5.9853 mL
	20 mM	0.1796 mL	0.8978 mL	1.7956 mL	4.4890 mL
	25 mM	0.1436 mL	0.7182 mL	1.4365 mL	3.5912 mL
	30 mM	0.1197 mL	0.5985 mL	1.1971 mL	2.9927 mL
	40 mM	0.0898 mL	0.4489 mL	0.8978 mL	2.2445 mL
	50 mM	0.0718 mL	0.3591 mL	0.7182 mL	1.7956 mL
	60 mM	0.0599 mL	0.2993 mL	0.5985 mL	1.4963 mL
	80 mM	0.0449 mL	0.2244 mL	0.4489 mL	1.1222 mL
	100 mM	0.0359 mL	0.1796 mL	0.3591 mL	0.8978 mL

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Dibenzyl trisulfide

Metabolism/Protease Compound Library

Natural Product Library

Anti-Cancer Compound Library

Traditional Chinese Medicine Active Compound Library

Angiogenesis-Related Compound Library

Anti-Cancer Natural Product Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

All Cathepsin Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Dibenzyl trisulfide

Metabolism/Protease Compound Library

Natural Product Library

Anti-Cancer Compound Library

Traditional Chinese Medicine Active Compound Library

Angiogenesis-Related Compound Library

Anti-Cancer Natural Product Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

All Cathepsin Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Dibenzyl trisulfide Related Classifications

Cat. No.: HY-129461