Cat. No.: HY-W018197

2-Phenylacetamide

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Based on 1 Customer Validation

2-Phenylacetamide is a estrogen-like compound that can be isolated from Lepidium apetalum seeds. 2-Phenylacetamide inhibits p38 MAPK signaling pathway, exhibits anti-inflammatory, antioxidant, anti-hypertensive, and anti-fibrosis effects. 2-Phenylacetamide is orally active.

For research use only. We do not sell to patients.

Purity: 99.92%
CAS No.: 103-81-1
Formula: C₈H₉NO
Molecular Weight:135.17

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

2-Phenylacetamide Chemical Structure

CAS No.: 103-81-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

All p38 MAPK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
BV-2	IC₅₀	>100 μM Compound: 7	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay	[PMID: 27919656]
Huh-7	CC₅₀	>100 μM Compound: GNF-Pf-1199	NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7) NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)	[PMID: 18579783]

In Vitro

2-Phenylacetamide (4-8 μM, 24 h) promotes the proliferation of cancer cell MCF-7, increases expressions of ERα, ERβ and GPR30 in MCF-7^[1].
2-Phenylacetamide (1-10 μM, 6 h) induces ROS generation, inhibits the activation of Renin-angiotensin-aldosterone system (RAAS) system and cytokine release, and reduces the expression of Smad3 and TGF-β in high NaCl-stimulated NRK52e cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

MCF-7
Concentration:

4-8 μM
Incubation Time:

24 h
Result:

Improved the cell viability of MCF-7.

Immunofluorescence^[1]

Cell Line:

NRK52e
Concentration:

1-10 μM
Incubation Time:

6 h
Result:

Reduced the expression of Smad3 and TGF-β.

In Vivo

2-Phenylacetamide (25-50 mg/kg, po, 7 days) increases the uterine coefficient, luteinizing hormone (LH) and estrogen (E2) levels in immature mice^[1].
2-Phenylacetamide (15-45 mg/kg, po, once daily for 4 weeks) improves kidney damage caused by hypertension, lowers blood pressure, and reduces kidney pathological damage and collagen deposition in rats spontaneously hypertensive models^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Immature female Swiss mice^[1]
Dosage:

25-50 mg/kg
Administration:

po, 7 days
Result:

Increased the uterine coefficient, LH and E2 levels of immature mice, increased the expression of ERα, ERβ and GPR30 in the uterus.

Animal Model:

Rats spontaneously hypertensive models^[2]
Dosage:

15-45 mg/kg
Administration:

po, once daily for 4 weeks
Result:

Reduced the blood pressure, increased the urine volume of SHR rats.
Improved the pathological structure of renal tissue, reduced collagen deposition and collagen volume fraction (CVF).

Chemical Information

CAS No. 103-81-1
Appearance Solid
Molecular Weight 135.17
Formula C₈H₉NO
Color White to off-white
SMILES

O=C(N)CC1=CC=CC=C1
Structure Classification
- Others
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (739.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

7.3981 mL

36.9905 mL

73.9809 mL

5 mM

1.4796 mL

7.3981 mL

14.7962 mL

10 mM

0.7398 mL

3.6990 mL

7.3981 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (18.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (18.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (18.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zeng M, et al., 2-Phenylacetamide Isolated from the Seeds of Lepidium apetalum and Its Estrogen-Like Effects In Vitro and In Vivo. Molecules. 2018 Sep 7;23(9):2293. [Content Brief]

[2]. Yuan PP, et al., 2-phenylacetamide Separated from the seed of Lepidium apetalum Willd. inhibited renal fibrosis via MAPK pathway mediated RAAS and oxidative stress in SHR Rats. BMC Complement Med Ther. 2023 Jun 23;23(1):207. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	7.3981 mL	36.9905 mL	73.9809 mL	184.9523 mL
	5 mM	1.4796 mL	7.3981 mL	14.7962 mL	36.9905 mL
	10 mM	0.7398 mL	3.6990 mL	7.3981 mL	18.4952 mL
	15 mM	0.4932 mL	2.4660 mL	4.9321 mL	12.3302 mL
	20 mM	0.3699 mL	1.8495 mL	3.6990 mL	9.2476 mL
	25 mM	0.2959 mL	1.4796 mL	2.9592 mL	7.3981 mL
	30 mM	0.2466 mL	1.2330 mL	2.4660 mL	6.1651 mL
	40 mM	0.1850 mL	0.9248 mL	1.8495 mL	4.6238 mL
	50 mM	0.1480 mL	0.7398 mL	1.4796 mL	3.6990 mL
	60 mM	0.1233 mL	0.6165 mL	1.2330 mL	3.0825 mL
	80 mM	0.0925 mL	0.4624 mL	0.9248 mL	2.3119 mL
	100 mM	0.0740 mL	0.3699 mL	0.7398 mL	1.8495 mL

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2-Phenylacetamide

Immunology/Inflammation Compound Library

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All Endogenous Metabolite Isoforms

All p38 MAPK Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

2-Phenylacetamide Related Classifications

HY-W018197 Related Classifications