Methyl vanillate
Based on 2 publication(s) in Google Scholar
Methyl vanillate, one of the ingredients in Oryza sativa Linn., is a Wnt/β-catenin pathway activator. A benzoate ester that is the methyl ester of vanillic acid. It has a role as an antioxidant and a plant metabolite.
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- Pureza : 99.88%
- No. CAS: 3943-74-6
- Fòrmula: C9H10O4
- Peso molecular:182.18
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Almacenamiento:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Methyl vanillate
MoreVer todos los productos específicos de isoformas Endogenous Metabolite
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Actividad biológica
Descripciòn
IC50 & Target
Wnt/β-catenin[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
>100 μM
Compound: 1bMe
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Anticancer activity against human A549 cells after 24 hrs by MTT assay
Anticancer activity against human A549 cells after 24 hrs by MTT assay
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[PMID: 20466556] |
| B16-F10 | IC50 |
>100 μM
Compound: 1bMe
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Anticancer activity against mouse B16F10 cells after 24 hrs by MTT assay
Anticancer activity against mouse B16F10 cells after 24 hrs by MTT assay
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[PMID: 20466556] |
| Hs 683 | IC50 |
>100 μM
Compound: 1bMe
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Anticancer activity against human Hs683 cells after 24 hrs by MTT assay
Anticancer activity against human Hs683 cells after 24 hrs by MTT assay
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[PMID: 20466556] |
| LoVo | IC50 |
83 μM
Compound: 1bMe
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Anticancer activity against human LoVo cells after 24 hrs by MTT assay
Anticancer activity against human LoVo cells after 24 hrs by MTT assay
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[PMID: 20466556] |
| MCF7 | IC50 |
>100 μM
Compound: 1bMe
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Anticancer activity against human MCF7 cells after 24 hrs by MTT assay
Anticancer activity against human MCF7 cells after 24 hrs by MTT assay
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[PMID: 20466556] |
| OE33 | IC50 |
>100 μM
Compound: 1bMe
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Anticancer activity against human OE33 cells after 24 hrs by MTT assay
Anticancer activity against human OE33 cells after 24 hrs by MTT assay
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[PMID: 20466556] |
| PC-3 | IC50 |
>100 μM
Compound: 1bMe
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Anticancer activity against human PC3 cells after 24 hrs by MTT assay
Anticancer activity against human PC3 cells after 24 hrs by MTT assay
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[PMID: 20466556] |
| RBL-2H3 | IC50 |
640 μM
Compound: 1
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Anti-allergic activity in RBL2H3 cells assessed as inhibition of anti-DNP-IgE-induced degranulation incubated for 20 mins followed by DNP-labeled human serum albumin addition measured after 1 hr by beta-hexosaminidase release assay
Anti-allergic activity in RBL2H3 cells assessed as inhibition of anti-DNP-IgE-induced degranulation incubated for 20 mins followed by DNP-labeled human serum albumin addition measured after 1 hr by beta-hexosaminidase release assay
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[PMID: 27324979] |
| T98G | IC50 |
>100 μM
Compound: 1bMe
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Anticancer activity against human T98G cells after 24 hrs by MTT assay
Anticancer activity against human T98G cells after 24 hrs by MTT assay
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[PMID: 20466556] |
| U-373MG ATCC | IC50 |
>100 μM
Compound: 1bMe
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Anticancer activity against human U373 cells after 24 hrs by MTT assay
Anticancer activity against human U373 cells after 24 hrs by MTT assay
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[PMID: 20466556] |
In Vitro
Methyl vanillate (0-100 μM) increases the expression and nuclear translocation of β-catenin in calvarial osteoblasts. Methyl vanillate dose-dependently elevates ALP activity in calvarial osteoblasts[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Chemical Information
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No. CAS 3943-74-6
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Appearance Solid
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Peso molecular 182.18
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Fòrmula C9H10O4
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Color Off-white to light brown
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SMILES
O=C(OC)C(C=C1)=CC(OC)=C1O
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (2)
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Journal Impact Factor
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Most Recent
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Cell Death Dis
The anthelmintic drug niclosamide induces GSK-β-mediated β-catenin degradation to potentiate gemcitabine activity, reduce immune evasion ability and suppress pancreatic cancer progression. [Abstract]2022 Feb 3;13(2):112. PMID: 35115509 -
Med Oncol
FAT4 activation inhibits epithelial-mesenchymal transition (EMT) by promoting autophagy in H2228/Cer cells. [Abstract]2022 Dec 28;40(1):64. PMID: 36576661
Solvente y solubilidad
In Vitro:
DMSO : ≥ 100 mg/mL (548.91 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 1.67 mg/mL (9.17 mM; Need ultrasonic)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.72 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.72 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 2 mg/mL (10.98 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (283 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 5.4891 mL | 27.4454 mL | 54.8908 mL | 137.2269 mL |
| 5 mM | 1.0978 mL | 5.4891 mL | 10.9782 mL | 27.4454 mL | |
| DMSO | 10 mM | 0.5489 mL | 2.7445 mL | 5.4891 mL | 13.7227 mL |
| 15 mM | 0.3659 mL | 1.8297 mL | 3.6594 mL | 9.1485 mL | |
| 20 mM | 0.2745 mL | 1.3723 mL | 2.7445 mL | 6.8613 mL | |
| 25 mM | 0.2196 mL | 1.0978 mL | 2.1956 mL | 5.4891 mL | |
| 30 mM | 0.1830 mL | 0.9148 mL | 1.8297 mL | 4.5742 mL | |
| 40 mM | 0.1372 mL | 0.6861 mL | 1.3723 mL | 3.4307 mL | |
| 50 mM | 0.1098 mL | 0.5489 mL | 1.0978 mL | 2.7445 mL | |
| 60 mM | 0.0915 mL | 0.4574 mL | 0.9148 mL | 2.2871 mL | |
| 80 mM | 0.0686 mL | 0.3431 mL | 0.6861 mL | 1.7153 mL | |
| 100 mM | 0.0549 mL | 0.2745 mL | 0.5489 mL | 1.3723 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Methyl vanillate Related Classifications
HY-75342 Related Classifications
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