dTAG-47
Based on 1 publication(s) in Google Scholar
dTAG-47 is a heterobifunctional PROTAC degrader that specifically binds to the FKBP12F36V domain and Cereblon (CRBN). By recruiting the CRBN E3 ubiquitin ligase, dTAG-47 induces ubiquitination and proteasomal degradation of FKBP12F36V-fused Cas9 and tagged KDM5A. dTAG-47 can induce upregulated expression of some endogenous retrovirus (ERV) genes and is suitable for cancer research.
(Pink: FKBP12 ligand (HY-114420); Blue: Cereblon ligand (HY-W087383); Black: linker (HY-42773)).
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- Pureza: 99.23%
- No. CAS: 2265886-81-3
- Fòrmula: C59H73N5O14
- Peso molecular:1076.24
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Almacenamiento:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) dTAG-47
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Actividad biológica
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FKBP12 |
Cereblon |
dTAG-47 (1-1000 nM; 48 h) dose-dependently inhibits NL-FKBP-Cas9-mediated eGFP disruption in U2OS.eGFP.PEST cells, eliminating activity at 100 nM[1].
dTAG-47 (0-3000 nM; 48 h) dose-dependently reduces NL-FKBP-Cas9-mediated HDR activity in HEK293T CRBN+/+ cells, with no effect in CRBN−/− cells, demonstrating CRBN-dependent Cas9 degradation[1].
dTAG-47 (0-1000 nM; 48 h) dose-dependently inhibits NL-FKBP-Cas9-mediated indel formation in Drosophila S2 cells[1].
dTAG-47 (1 μM; added at 0.5, 1, 2, 4, 8, 12, 24, 48 h post-transfection, with total incubation through 72 h post-transfection) modulates NL-FKBP-Cas9 lifetime to regulate DNA repair, with HDR saturating at 24 h and NHEJ accumulating with prolonged Cas9 activity in HEK293T cells targeted at the RBM20 locus[1].
dTAG-47 (1 μM; added at 0, 0.5, 1, 2, 4, 8, 12, 24, 48 h post-transfection, with total incubation through 72 h post-transfection) modulates NL-FKBP-Cas9 lifetime, with HDR activity saturating at 24 h of Cas9 activity in HEK293T cells targeted at the GAPDH locus[1].
dTAG-47 (0-1000 nM; 72 h) dose-dependently enhances the on-target specificity of NL-FKBP-Cas9 targeting EMX1 in HEK293T cells, increasing the on-target/off-target indel ratio[1].
dTAG-47 (0.1-1 µM; 48 h) treatment of HAP1dTAG-KDM5A cells for 48 h increases transcript levels of ERV3-1, ERVV-2, ERVW, and HERVE, but does not significantly alter ZNF208 or ZNF676 transcript levels[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HAP1dTAG-KDM5A cells (HAP1 cells with FKBP12F36V-2xHA-tagged KDM5A)
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Concentration:0.1 µM (48 h incubation); 1 µM (48 h incubation)
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Incubation Time:48 h
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Result:Increased transcript levels of ERV3-1, ERVV-2, ERVW, and HERVE.
Caused no significant changes in the levels of ZNF208 or ZNF676 transcripts.
Chemical Information
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No. CAS 2265886-81-3
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Appearance Solid
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Peso molecular 1076.24
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Fòrmula C59H73N5O14
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Color Light yellow to green yellow
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SMILES
O=C1N(C2C(NC(CC2)=O)=O)C(C3=CC=C(NCCCCCCCCNC(COC4=C(C=CC=C4)[C@@H](CCC5=CC(OC)=C(C=C5)OC)OC([C@H]6N(CCCC6)C([C@H](C7=CC(OC)=C(C(OC)=C7)OC)CC)=O)=O)=O)C=C31)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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bioRxiv
2025 Nov 30:2025.11.26.690562. PMID: 41394586
Solvente y solubilidad
DMSO : 100 mg/mL (92.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.9292 mL | 4.6458 mL | 9.2916 mL | 23.2290 mL |
| 5 mM | 0.1858 mL | 0.9292 mL | 1.8583 mL | 4.6458 mL | |
| 10 mM | 0.0929 mL | 0.4646 mL | 0.9292 mL | 2.3229 mL | |
| 15 mM | 0.0619 mL | 0.3097 mL | 0.6194 mL | 1.5486 mL | |
| 20 mM | 0.0465 mL | 0.2323 mL | 0.4646 mL | 1.1615 mL | |
| 25 mM | 0.0372 mL | 0.1858 mL | 0.3717 mL | 0.9292 mL | |
| 30 mM | 0.0310 mL | 0.1549 mL | 0.3097 mL | 0.7743 mL | |
| 40 mM | 0.0232 mL | 0.1161 mL | 0.2323 mL | 0.5807 mL | |
| 50 mM | 0.0186 mL | 0.0929 mL | 0.1858 mL | 0.4646 mL | |
| 60 mM | 0.0155 mL | 0.0774 mL | 0.1549 mL | 0.3872 mL | |
| 80 mM | 0.0116 mL | 0.0581 mL | 0.1161 mL | 0.2904 mL |
dTAG-47 Related Classifications
HY-147098 Related Classifications
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