FLS-359
Based on 1 Customer Validation
FLS-359 is a selective, orally active allosteric modulator for sirtuin 2, with the IC50 of 3 μM. FLS-359 exhibits antiviral activity against RNA and DNA virus, through inhibition of DNA/RNA replication.
For research use only. We do not sell to patients.
- Purity: 95.41%
- CAS No.: 2309398-79-4
- Formula: C27H26N6S
- Molecular Weight:466.60
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Anti-Infection Compound Library
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Cell Cycle/DNA Damage Compound Library
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Epigenetics Compound Library
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Histone Modification Research Compound Library
HY-L024
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Antiviral Compound Library
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Anti-Aging Compound Library
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Orally Active Compound Library
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Glutamine Metabolism Compound Library
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Metabolic Enzyme Compound Library
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Allosteric Modulators (AMs) Compound Library
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Radioprotector Library
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Bioactive Compound Library Max
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Cell Proliferation Compound Library
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High-Throughput Bioactive Compound Library
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Biological Activity
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SIRT2 3 μM (IC50) |
FLS-359 (0-100 μM) inhibits deacetylation of SIRT2 on a histone H3 site, with a substrate selectivity[1].
FLS-359 inhibits growth of multiple RNA and DNA virus, like SARS-CoV-2, Influenza A, Hepatitis B, respiratory syncytial virus, with IC50 range of 0.3-6.7 μM[1].
FLS-359 (0-10 μM, 24 h) inhibits the lytic activation of Epstein-Barr virus (EBV) in cells Akata, and the spread of human cytomegalovirus (HCMV) in diploid fibroblast with IC50 of 0.466 μM[1].
FLS-359 (0-5 μM, 72 h) inhibits the accumulation of intracellular viral nucleic acids, the production of extracellular virus particles, and the infectivity of the virus particles[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
FLS-359 (50 mg/kg, p.o., twice daily for 11 days) inhibits HCMV infection in immunodeficient mice model[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mouse gelfoam-fibroblast model and Human lung-only mouse model[1]
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Dosage:50 mg/kg
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Administration:p.o., two time a day for 11 days
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Result:Inhibited infection of HCMV.
Chemical Information
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CAS No. 2309398-79-4
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Appearance Solid
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Molecular Weight 466.60
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Formula C27H26N6S
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Color White to off-white
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SMILES
CC1=CN(C(C)=N1)C2=CC=C3CCN(CC3=C2)C4=NC=C(S4)CC5=CC=C(C=C5)N6C=CC=N6
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (214.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.36 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.36 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1432 mL | 10.7158 mL | 21.4316 mL | 53.5791 mL |
| 5 mM | 0.4286 mL | 2.1432 mL | 4.2863 mL | 10.7158 mL | |
| 10 mM | 0.2143 mL | 1.0716 mL | 2.1432 mL | 5.3579 mL | |
| 15 mM | 0.1429 mL | 0.7144 mL | 1.4288 mL | 3.5719 mL | |
| 20 mM | 0.1072 mL | 0.5358 mL | 1.0716 mL | 2.6790 mL | |
| 25 mM | 0.0857 mL | 0.4286 mL | 0.8573 mL | 2.1432 mL | |
| 30 mM | 0.0714 mL | 0.3572 mL | 0.7144 mL | 1.7860 mL | |
| 40 mM | 0.0536 mL | 0.2679 mL | 0.5358 mL | 1.3395 mL | |
| 50 mM | 0.0429 mL | 0.2143 mL | 0.4286 mL | 1.0716 mL | |
| 60 mM | 0.0357 mL | 0.1786 mL | 0.3572 mL | 0.8930 mL | |
| 80 mM | 0.0268 mL | 0.1339 mL | 0.2679 mL | 0.6697 mL | |
| 100 mM | 0.0214 mL | 0.1072 mL | 0.2143 mL | 0.5358 mL |
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