1. Metabolic Enzyme/Protease Immunology/Inflammation NF-κB Apoptosis
  2. Angiotensin-converting Enzyme (ACE) Toll-like Receptor (TLR) NF-κB TNF Receptor Interleukin Related
  3. Fosfenopril

Fosfenopril  (Synonyms: Fosinoprilat; Fosinoprilic acid; SQ 27519)

Cat. No.: HY-107352 Purity: 99.89%
COA Handling Instructions

Fosfenopril (Fosinoprilat) is a potent angiotensin converting enzyme (ACE) inhibitor. Fosfenopril alleviates lipopolysaccharide (LPS)-induced inflammation by inhibiting TLR4/NF-κB signaling in monocytes.

For research use only. We do not sell to patients.

Fosfenopril Chemical Structure

Fosfenopril Chemical Structure

CAS No. : 95399-71-6

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 364 In-stock
Solution
10 mM * 1 mL in DMSO USD 364 In-stock
Solid
1 mg USD 128 In-stock
5 mg USD 380 In-stock
10 mg USD 620 In-stock
25 mg USD 1230 In-stock
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Fosfenopril (Fosinoprilat) is a potent angiotensin converting enzyme (ACE) inhibitor. Fosfenopril alleviates lipopolysaccharide (LPS)-induced inflammation by inhibiting TLR4/NF-κB signaling in monocytes[1][2].

IC50 & Target

TLR4

 

NF-κB

 

IL-6

 

IL-1β

 

In Vitro

Fosfenopril (0-10 μM, 5 min) inhibits the expression of TLR4 which is elevated by LPS[1].
Fosfenopril (0-10 μM, 1 h)attenuates the expression of NF-κB which is activated by LPS[1].
Fosfenopril inhibits LPS-induced cytokines (IL-6, IL-1β, and TNF-α) expression[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: THP1 cells
Concentration: 0, 0.25, 0.5, 1, 5, and 10 μM
Incubation Time: 1 h
Result: Down-regulated the expression of NF-κB protein.

RT-PCR[1]

Cell Line: THP1 cells
Concentration: 0, 0.25, 0.5, 1, 5, and 10 μM
Incubation Time: 5 min
Result: Showed a dose-dependent inhibitory effect on the TLR4 expression.
In Vivo

Fosfenopril (0.5 mg/kg bolus plus 0.1 mg/kg/min, IV, once) increases p-aminohippurate (PAH) clearance and glomerular filtration rate (GFR) in dogs[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Famale mongrel dogs (n=7)[3]
Dosage: 0.5 mg/kg (1.1 mumol/kg) bolus plus 0.1 mg/kg/min (0.22 mumol/kg/min)
Administration: IV, once
Result: Increased p-aminohippurate (PAH) clearance and glomerular filtration rate (GFR) by 25 and 16%, respectively without changing arterial pressure (AP).
Molecular Weight

435.49

Formula

C23H34NO5P

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=P(CCCCC1=CC=CC=C1)(O)CC(N2C[C@H](C3CCCCC3)C[C@H]2C(O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (229.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2963 mL 11.4813 mL 22.9626 mL
5 mM 0.4593 mL 2.2963 mL 4.5925 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

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Concentration (final)

C2

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V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.74 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.74 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2963 mL 11.4813 mL 22.9626 mL 57.4066 mL
5 mM 0.4593 mL 2.2963 mL 4.5925 mL 11.4813 mL
10 mM 0.2296 mL 1.1481 mL 2.2963 mL 5.7407 mL
15 mM 0.1531 mL 0.7654 mL 1.5308 mL 3.8271 mL
20 mM 0.1148 mL 0.5741 mL 1.1481 mL 2.8703 mL
25 mM 0.0919 mL 0.4593 mL 0.9185 mL 2.2963 mL
30 mM 0.0765 mL 0.3827 mL 0.7654 mL 1.9136 mL
40 mM 0.0574 mL 0.2870 mL 0.5741 mL 1.4352 mL
50 mM 0.0459 mL 0.2296 mL 0.4593 mL 1.1481 mL
60 mM 0.0383 mL 0.1914 mL 0.3827 mL 0.9568 mL
80 mM 0.0287 mL 0.1435 mL 0.2870 mL 0.7176 mL
100 mM 0.0230 mL 0.1148 mL 0.2296 mL 0.5741 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Fosfenopril
Cat. No.:
HY-107352
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