ST7710AA1
ST7710AA1 (compound 1l) is a potent PARP-1 inhibitor with an IC50 value of 0.07 µM. ST7710AA1 shows an antiproliferative activity. ST7710AA1 shows anticancer activity.
For research use only. We do not sell to patients.
- CAS No.: 1542067-20-8
- Formula: C20H22N4O
- Molecular Weight:334.41
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
PARP-1 0.07 μM (IC50) |
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A2780 | IC50 |
2.9 μM
Compound: 1l, ST7710AA1
|
Antiproliferative activity against human A2780 cells after 72 hrs by SRB assay
Antiproliferative activity against human A2780 cells after 72 hrs by SRB assay
|
[PMID: 24398383] |
| CAPAN-1 | IC50 |
22.04 μM
Compound: 1l, ST7710AA1
|
Antiproliferative activity against BRCA2 gene mutated human Capan1 cells after 72 hrs by SRB assay
Antiproliferative activity against BRCA2 gene mutated human Capan1 cells after 72 hrs by SRB assay
|
[PMID: 24398383] |
| HCC1937 | IC50 |
19.43 μM
Compound: 1l, ST7710AA1
|
Antiproliferative activity against BRCA1 gene mutated human HCC1937 cells after 72 hrs by SRB assay
Antiproliferative activity against BRCA1 gene mutated human HCC1937 cells after 72 hrs by SRB assay
|
[PMID: 24398383] |
| HeLa | EC50 |
400 nM
Compound: 1l, ST7710AA1
|
Inhibition of PARP-1 in human HeLa cells assessed as decrease of H2O2-induced PARylation after 3 hrs by fluorescence assay
Inhibition of PARP-1 in human HeLa cells assessed as decrease of H2O2-induced PARylation after 3 hrs by fluorescence assay
|
[PMID: 24398383] |
In Vitro
ST7710AA1 (compound 1l) (0-200 µM; 72 h) shows antiproliferative with IC50s of 19.43, 22.04 µM for HCC1937, Capan 1, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 1542067-20-8
-
Molecular Weight 334.41
-
Formula C20H22N4O
-
SMILES
O=C(N1C=CC2=C1N=C(C=C2)C3=CC=C(C=C3)CN4CCCCC4)N
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
-
Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
-
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
ST7710AA1 Related Classifications
HY-117423 Related Classifications
Powered by Bioz