Magainin 2
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Magainin 2 (Magainin II) is an antimicrobial peptide (AMP) isolated from the skin of the African clawed frog Xenopus laevis. Magainin 2 displays antibiotic activity against numerous gram-negative and gram-positive bacteria. Magainin 2 also is active against protozoa. Magainin 2 exerts its cytotoxicity effects by preferential interactions with anionic phospholipids abundant in bacterial membranes.
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- Pureté: 98.75%
- CAS No.: 108433-95-0
- Formule: C114H180N30O29S
- Masse moléculaire:2466.90
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Stockage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Magainin 2 exhibits bactericidal effects and induces morphological changes in Escherichia coli regarding early apoptosis. Magainin 2 induces the expression of a bacterial protein with affinity for the caspase substrate and effects the expression of RecA as a caspase-like protein[1]. Magainin 2 kill bacteria by permeabilizing the cell membranes without exhibiting significant toxicity against mammalian cells. The main target of the peptide is considered to be the lipid matrix of the membranes. Application of 10 μg /mL magainin 2 to Paramecium caudatum, a protozoan, in pond water caused an osmotic swelling of the cell and a subsequent cell burst, suggesting that the peptide could perturb membrane functions responsible for osmotic balance[2]. Magainin 2 permeabilizes bacterial and mammalian membranes in significantly different ways. The peptide forms pores with a diameter of about 2.8 nm (less than 6.6 nm) in B. megaterium, and translocates into the cytosol. In contrast, the peptide significantly perturbes the membrane of CHO-K1 cells, permitting the entry of a large molecule (larger than 23 nm) into the cytosol, accompanied by membrane budding and lipid flip-flop, mainly accumulating in mitochondria and nuclei[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 108433-95-0
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Appearance Solid
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Masse moléculaire 2466.90
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Formule C114H180N30O29S
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Color White to off-white
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Synonyms
Magainin II
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Sequence
Gly-Ile-Gly-Lys-Phe-Leu-His-Ser-Ala-Lys-Lys-Phe-Gly-Lys-Ala-Phe-Val-Gly-Glu-Ile-Met-Asn-Ser
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Sequence Shortening
GIGKFLHSAKKFGKAFVGEIMNS
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Structure Classification
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Initial Source
the skin of the African clawed frog
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvant et solubilité
H2O : 50 mg/mL (20.27 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
Escherichia coli cells are incubated with magainin 2 (50 μg /mL). The cultures are acquired after incubation for 0, 2, 4, 6, and 8 h, respectively, and spread onto LB agar plates, and then the colony-forming units are counted after incubation for 24 h at 37°C. The percentage survival is determined relative to the control treatment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureté et documentation
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Fiche technique (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. M Zasloff, et al. Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5449-53. [Content Brief]
[2]. K Matsuzaki, et al. Magainins as paradigm for the mode of action of pore forming polypeptides. Biochim Biophys Acta. 1998 Nov 10;1376(3):391-400. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.4054 mL | 2.0268 mL | 4.0537 mL | 10.1342 mL |
| 5 mM | 0.0811 mL | 0.4054 mL | 0.8107 mL | 2.0268 mL | |
| 10 mM | 0.0405 mL | 0.2027 mL | 0.4054 mL | 1.0134 mL | |
| 15 mM | 0.0270 mL | 0.1351 mL | 0.2702 mL | 0.6756 mL | |
| 20 mM | 0.0203 mL | 0.1013 mL | 0.2027 mL | 0.5067 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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