Nithiamide
Based on 2 publication(s) in Google Scholar
Nithiamide is a non-5-nitroimidazole drugs, is a antibiotic used in veterinary.
For research use only. We do not sell to patients.
- Purity: 99.74%
- CAS No.: 140-40-9
- Formula: C5H5N3O3S
- Molecular Weight:187.18
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Nithiamide
MoreAll Antibiotic Isoforms
More
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Vero | CC50 |
>1000 μM
Compound: 9
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Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay
Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay
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[PMID: 24529307] |
| Vero | CC50 |
>1000 μM
Compound: Aminitrozole
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Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay
Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay
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[PMID: 25801157] |
Chemical Information
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CAS No. 140-40-9
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Appearance Solid
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Molecular Weight 187.18
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Formula C5H5N3O3S
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Color Light yellow to khaki
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SMILES
CC(NC1=NC=C([N+]([O-])=O)S1)=O
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Synonyms
CL-5279; Aminitrozole
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (2)
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Journal Impact Factor
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Most Recent
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ACS Omega
2020 Oct 12;5(41):26551-26561. PMID: 33110983 -
Solvent & Solubility
DMSO : 100 mg/mL (534.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.36 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.36 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.3425 mL | 26.7123 mL | 53.4245 mL | 133.5613 mL |
| 5 mM | 1.0685 mL | 5.3425 mL | 10.6849 mL | 26.7123 mL | |
| 10 mM | 0.5342 mL | 2.6712 mL | 5.3425 mL | 13.3561 mL | |
| 15 mM | 0.3562 mL | 1.7808 mL | 3.5616 mL | 8.9041 mL | |
| 20 mM | 0.2671 mL | 1.3356 mL | 2.6712 mL | 6.6781 mL | |
| 25 mM | 0.2137 mL | 1.0685 mL | 2.1370 mL | 5.3425 mL | |
| 30 mM | 0.1781 mL | 0.8904 mL | 1.7808 mL | 4.4520 mL | |
| 40 mM | 0.1336 mL | 0.6678 mL | 1.3356 mL | 3.3390 mL | |
| 50 mM | 0.1068 mL | 0.5342 mL | 1.0685 mL | 2.6712 mL | |
| 60 mM | 0.0890 mL | 0.4452 mL | 0.8904 mL | 2.2260 mL | |
| 80 mM | 0.0668 mL | 0.3339 mL | 0.6678 mL | 1.6695 mL | |
| 100 mM | 0.0534 mL | 0.2671 mL | 0.5342 mL | 1.3356 mL |
Nithiamide Related Classifications
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