BAY-850
Based on 5 publication(s) in Google Scholar
BAY-850, a chemical probe, is a potent and isoform selective ATPase family AAA domain-containing protein 2 (ATAD2) inhibitor, with an IC50 of 166 nM.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.93%
- No. CAS: 2099142-76-2
- Fòrmula: C38H44ClN5O3
- Peso molecular:654.24
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Solvente y solubilidad
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Publications Citing Use of MedChemExpress (MCE) BAY-850
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Actividad biológica
ATAD2[1].
BAY-850 competes with the binding of a mono-acetylated Histone H4 N-terminal peptide to ATAD2 BD with an IC50 of 166 nM measured in TR-FRET assay. BAY-850 displaces the tetra-acetylated peptide with an IC50 of 157 nM and a KD of 115 nM respectively. The unprecedented isoform selectivity of BAY-850 suggests a different mode of action to those exhibited by canonical BD inhibitors[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 2099142-76-2
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Appearance Solid
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Peso molecular 654.24
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Fòrmula C38H44ClN5O3
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Color White to light yellow
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SMILES
O=C(N[C@H](CC1=CC=C(C#N)C=C1)CN[C@H]2CC[C@@H](N)CC2)C3=CC(C4=CC=C(CN[C@@H](C5=CC=C(C)C=C5)C)O4)=C(OC)C=C3Cl
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (5)
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Journal Impact Factor
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Most Recent
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Nat Commun
Human iPSC-based Modeling of Pulmonary Fibrosis Reveals p300/CBP Inhibition Suppresses Alveolar Transitional Cell State. [Abstract]2026 Feb 12;17(1):1214. PMID: 41680175 -
J Enzyme Inhib Med Chem
Fragment-based design, synthesis and biological evaluation of theophylline derivatives as ATAD2 inhibitors in BT-549 cells. [Abstract]2023 Dec;38(1):2242601. PMID: 37533352
BAY-850 purchased from MedChemExpress. Usage Cited in: J Enzyme Inhib Med Chem. 2023 Dec;38(1):2242601. [Abstract]
The effect of AM879, BAY-850 and compound 19f on ATAD2 activity detected by TR-FRET technology.
BAY-850 purchased from MedChemExpress. Usage Cited in: J Enzyme Inhib Med Chem. 2023 Dec;38(1):2242601. [Abstract]
The effect of AM879, BAY-850 (10, 20, 30, 40, 50 μM), and compound 19f on the cell viability of BT-549 cells.
BAY-850 purchased from MedChemExpress. Usage Cited in: J Enzyme Inhib Med Chem. 2023 Dec;38(1):2242601. [Abstract]
BT-549 cells were treated with 10 μM AM879, BAY-850 or compound 19f for 48 h, the expression level of p-c-Myc Ser62 was detected by immunofluorescence.
BAY-850 purchased from MedChemExpress. Usage Cited in: J Enzyme Inhib Med Chem. 2023 Dec;38(1):2242601. [Abstract]
BT-549 cells were treated with 5, 10, 20 μM AM879, BAY-850, or compound 19f for 48 h, the expression level of ATAD2, c-Myc, and p-c-MycSer62 were detected by western blot.
BAY-850 purchased from MedChemExpress. Usage Cited in: J Enzyme Inhib Med Chem. 2023 Dec;38(1):2242601. [Abstract]
Colony formation assay were used to detect the long-term cell proliferation ability after compound 19f, BAY-850 (10 μM) and AM879 treatment.
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Commun Biol
A genome-scale CRISPR Cas9 dropout screen identifies synthetically lethal targets in SRC-3 inhibited cancer cells. [Abstract]2021 Mar 25;4(1):399. PMID: 33767353 -
Discov Oncol
ATAD2 promotes glycolysis and tumor progression in clear cell renal cell carcinoma by regulating the transcriptional activity of c-Myc. [Abstract]2023 May 26;14(1):79. PMID: 37233956 -
Eur Rev Med Pharmacol Sci
MiR-200b-5p inhibits proliferation of ovarian cancer cells by targeting ATAD2 and regulating PI3K/AKT signaling pathway. [Abstract]2020 Oct;24(19):9860-9868. PMID: 33090389
Solvente y solubilidad
DMSO : 62.5 mg/mL (95.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (7.64 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5285 mL | 7.6425 mL | 15.2849 mL | 38.2123 mL |
| 5 mM | 0.3057 mL | 1.5285 mL | 3.0570 mL | 7.6425 mL | |
| 10 mM | 0.1528 mL | 0.7642 mL | 1.5285 mL | 3.8212 mL | |
| 15 mM | 0.1019 mL | 0.5095 mL | 1.0190 mL | 2.5475 mL | |
| 20 mM | 0.0764 mL | 0.3821 mL | 0.7642 mL | 1.9106 mL | |
| 25 mM | 0.0611 mL | 0.3057 mL | 0.6114 mL | 1.5285 mL | |
| 30 mM | 0.0509 mL | 0.2547 mL | 0.5095 mL | 1.2737 mL | |
| 40 mM | 0.0382 mL | 0.1911 mL | 0.3821 mL | 0.9553 mL | |
| 50 mM | 0.0306 mL | 0.1528 mL | 0.3057 mL | 0.7642 mL | |
| 60 mM | 0.0255 mL | 0.1274 mL | 0.2547 mL | 0.6369 mL | |
| 80 mM | 0.0191 mL | 0.0955 mL | 0.1911 mL | 0.4777 mL |
BAY-850 Related Classifications
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