Orientin
Based on 7 publication(s) in Google Scholar
Orientin is a neuroprotective agentinhibits which has anti-inflammation, anti-oxidative, anti-tumor, and cardio protection properties. Orientin inhibits the levels of IL-6, IL-1β and TNF-α. Orientin increases IL-10 level. Orientin exhibits neuroprotective effect by inhibits TLR4 and NF-kappa B signaling pathway. Orientin can used in study neuropathic pain.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.89%
- No. CAS: 28608-75-5
- Fòrmula: C21H20O11
- Peso molecular:448.38
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Orientin
More- Adv Sci (Weinh). 2025 Oct 20:e12023. [Abstract]
- Apoptosis. 2022 Dec;27(11-12):1031-1048. [Abstract]
- Free Radic Biol Med. 2026 Mar 16:246:196-208. [Abstract]
- J Ethnopharmacol. 2027 Jan 10:372:122161.
- Food Sci Nutr. 2024 Jul 27;12(10):7461-7480. [Abstract]
- Vet Microbiol. 2026 May:316:110992. [Abstract]
- J Orthop Res. 2023 Nov;41(11):2405-2417. [Abstract]
Customer Validation & Images
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Cell Proliferation/Viability Assay
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IF
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Flow Cytometry
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WB
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In Vivo Efficacy Study
Ver todos los productos específicos de isoformas TNF Receptor
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Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| KB | ED50 |
25 μg/mL
Compound: NSC-133101
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Cytotoxicity against human KB cells
Cytotoxicity against human KB cells
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[PMID: 469554] |
In Vitro
Orientin (1 µM) inhibits allergic inflammation in RBL-2H3, mBMMCs, and RPMCs cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RBL-2H3, mBMMCs, and RPMCs
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Concentration:0.01–100 µM
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Incubation Time:8 h
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Result:Significantly suppressed the levels of histamine and β-hexosaminidase in RBL-2H3, mBMMCs, and RPMCs cells.
Inhibited the intracellular calcium levels in RBL-2H3, mBMMCs, and RPMCs cells.
Markedly inhibited the pro-inflammatory cytokines expression and secretion in RBL-2H3 cells.
Inhibited phosphorylation in RBL-2H3 cells.
In Vivo
Orientin (10–40 mg/kg for i.p.;once daily for 12 days) has an anti-neuroinflammatory effect in SNL rat model[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:PCA mouse model[1]
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Dosage:0.1–10 mg/kg
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Administration:Oral gavage (p.o.); Euthanized mouse after 1 h
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Result:Significantly suppressed PCA reactions in PCA mouse model.
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Animal Model:SNL rat model[2]
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Dosage:10, 20, 40 mg/kg
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Administration:Intraperitoneal injection (i.p.); Once daily for 12 days
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Result:Alleviated the downreg-ulation of PWL and promoted the behavior recovery in SNL rat model.
Decreased the levels of IL-6, IL-1β and TNF-α.
Increased the level of anti-inflammatory IL-10.
Down-regulated the level of MDA.
Chemical Information
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No. CAS 28608-75-5
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Appearance Solid
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Peso molecular 448.38
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Fòrmula C21H20O11
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Color Light yellow to yellow
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SMILES
O[C@@H]1[C@@H](O)[C@H](C2=C(O)C=C(O)C3=C2OC(C4=CC=C(O)C(O)=C4)=CC3=O)O[C@H](CO)[C@H]1O
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (7)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
2025 Oct 20:e12023. PMID: 41114468 -
Apoptosis
Orientin downregulating oxidative stress-mediated endoplasmic reticulum stress and mitochondrial dysfunction through AMPK/SIRT1 pathway in rat nucleus pulposus cells in vitro and attenuated intervertebral disc degeneration in vivo. [Abstract]2022 Dec;27(11-12):1031-1048. PMID: 36125665 -
Free Radic Biol Med
Orientin alleviates severe inflammation via regulating macrophage glycolysis and immune function in sepsis. [Abstract]2026 Mar 16:246:196-208. PMID: 41548767 -
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Food Sci Nutr
Orientin promotes diabetic wounds healing by suppressing ferroptosis via activation of the Nrf2/GPX4 pathway. [Abstract]2024 Jul 27;12(10):7461-7480. PMID: 39479645
Orientin purchased from MedChemExpress. Usage Cited in: Food Sci Nutr. 2024 Jul 27;12(10):7461-7480. [Abstract]
Percentage of viable cells after treatment with different concentrations of Orientin (5, 10, 20, 40, 80, 160 μM) for 24 or 48 h.
Orientin purchased from MedChemExpress. Usage Cited in: Food Sci Nutr. 2024 Jul 27;12(10):7461-7480. [Abstract]
Quantitative analysis of the DHE staining as well as representative images by treatment with Orientin (20 μM).
Orientin purchased from MedChemExpress. Usage Cited in: Food Sci Nutr. 2024 Jul 27;12(10):7461-7480. [Abstract]
The MitoSox (red) stain was used to conduct the MitoROS staining. The intensity of fluorescence as measured by flow cytometry in relation to its mean value by treatment with Orientin (20 μM).
Orientin purchased from MedChemExpress. Usage Cited in: Food Sci Nutr. 2024 Jul 27;12(10):7461-7480. [Abstract]
Western blot analysis comparing the levels of MFN1 and MFN2 expression in HUVECs by treatment with Orientin (20 μM).
Orientin purchased from MedChemExpress. Usage Cited in: Food Sci Nutr. 2024 Jul 27;12(10):7461-7480. [Abstract]
Representative digital images of wound closure for control and Orientin (20 mg/kg) group on day 0, day 5, day 10, and day 15.
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Vet Microbiol
The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]2026 May:316:110992. PMID: 41865607 -
J Orthop Res
Orientin inhibits inflammation in chondrocytes and attenuates osteoarthritis through Nrf2/NF-κB and SIRT6/NF-κB pathway. [Abstract]2023 Nov;41(11):2405-2417. PMID: 37186383
Solvente y solubilidad
In Vitro:
DMSO : 31.25 mg/mL (69.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.64 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.64 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (282 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.2303 mL | 11.1513 mL | 22.3025 mL | 55.7563 mL |
| 5 mM | 0.4461 mL | 2.2303 mL | 4.4605 mL | 11.1513 mL | |
| 10 mM | 0.2230 mL | 1.1151 mL | 2.2303 mL | 5.5756 mL | |
| 15 mM | 0.1487 mL | 0.7434 mL | 1.4868 mL | 3.7171 mL | |
| 20 mM | 0.1115 mL | 0.5576 mL | 1.1151 mL | 2.7878 mL | |
| 25 mM | 0.0892 mL | 0.4461 mL | 0.8921 mL | 2.2303 mL | |
| 30 mM | 0.0743 mL | 0.3717 mL | 0.7434 mL | 1.8585 mL | |
| 40 mM | 0.0558 mL | 0.2788 mL | 0.5576 mL | 1.3939 mL | |
| 50 mM | 0.0446 mL | 0.2230 mL | 0.4461 mL | 1.1151 mL | |
| 60 mM | 0.0372 mL | 0.1859 mL | 0.3717 mL | 0.9293 mL |
Orientin Related Classifications
HY-N0405 Related Classifications
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