Tiamulin
Based on 1 publication(s) in Google Scholar
Tiamulin (Thiamutilin) is a diterpenic antibiotic that is widely used in pigs and poultry for the control of infectious diseases. Tiamulin is effectively used in the study of airsacculitis, which is primarily caused by Mycoplasma spp.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.74%
- No. CAS: 55297-95-5
- Fòrmula: C28H47NO4S
- Peso molecular:493.74
-
Almacenamiento:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Pureza y Documentación
- Referencias
-
Help & FAQs
-
Anti-Infection Compound Library
HY-L002
-
Antibacterial Compound Library
HY-L049
-
FDA Approved & Pharmacopeial Drug Library
HY-L066
-
Antibiotics Library
HY-L067
-
Cysteine Targeted Covalent Library
HY-L153
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Natural Product-like Compound Library
HY-L021L
-
Natural Product and Natural Product-Like Compound Library
HY-L021M
-
Bioactive Compound Library
HY-L001
-
High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) Tiamulin
MoreVer todos los productos específicos de isoformas Antibiotic
More
Actividad biológica
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| HepG2 | IC50 |
>10 μM
Compound: Tiamulin
|
Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
|
[PMID: 35220163] |
| HGC-27 | IC50 |
>10 μM
Compound: Tiamulin
|
Cytotoxicity against human HGC-27 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
Cytotoxicity against human HGC-27 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
|
[PMID: 35220163] |
| HT-29 | IC50 |
>10 μM
Compound: Tiamulin
|
Cytotoxicity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
Cytotoxicity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
|
[PMID: 35220163] |
| MCF7 | IC50 |
>10 μM
Compound: Tiamulin
|
Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
|
[PMID: 35220163] |
| U-87MG ATCC | IC50 |
>10 μM
Compound: Tiamulin
|
Cytotoxicity against human U87 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
Cytotoxicity against human U87 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay
|
[PMID: 35220163] |
Tiamulin is a semisynthetic derivative of the diterpene antibiotic Pleuromutilin and is effectively used in the study of airsacculitis, which is primarily caused by Mycoplasma spp[1].
Tiamulin is highly active in vitro against Mycoplasma strains (M. gallisepticum, M. synoviae, M. meleagridis, and M. iowae), Spirochaetes (Brachyspira hyodysenteriae, Brachyspira innocens, B. pilosicoli, B. intermedia), gram-positive bacteria (staphylococci, streptococci, Clostridia, Arcanobacterium spp), but less active against gram-negative bacteria (Pasteurella, Klebsiella, Haemophilus, Fusobacterium, Campylobacter, Bacteroides spp.)[1].
Tiamulin binds with the rRNA in the peptidyl transferase slot on the ribosome, in which it prevents the correct positioning of the CCA ends of tRNA for peptide transferase and subsequent protein production[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
No. CAS 55297-95-5
-
Appearance Oil
-
Peso molecular 493.74
-
Fòrmula C28H47NO4S
-
Color Light yellow to brown
-
SMILES
O=C(O[C@H]1[C@@]([C@H](C)CC2)(C)[C@@](C(CC3)=O)([H])[C@]32[C@@H](C)[C@H](O)[C@](C)(C=C)C1)CSCCN(CC)CC
-
Synonyms
Thiamutilin
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Pathogens
Mycoplasma bovis Invades Non-Phagocytic Cells by Clathrin-Dependent Endocytic Pathways and Escapes from Phagocytic Vesicles. [Abstract]2024 Nov 15;13(11):1003. PMID: 39599557
Solvente y solubilidad
DMSO : 100 mg/mL (202.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (10.13 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
-
Ficha de datos (276 KB)
-
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0254 mL | 10.1268 mL | 20.2536 mL | 50.6339 mL |
| 5 mM | 0.4051 mL | 2.0254 mL | 4.0507 mL | 10.1268 mL | |
| 10 mM | 0.2025 mL | 1.0127 mL | 2.0254 mL | 5.0634 mL | |
| 15 mM | 0.1350 mL | 0.6751 mL | 1.3502 mL | 3.3756 mL | |
| 20 mM | 0.1013 mL | 0.5063 mL | 1.0127 mL | 2.5317 mL | |
| 25 mM | 0.0810 mL | 0.4051 mL | 0.8101 mL | 2.0254 mL | |
| 30 mM | 0.0675 mL | 0.3376 mL | 0.6751 mL | 1.6878 mL | |
| 40 mM | 0.0506 mL | 0.2532 mL | 0.5063 mL | 1.2658 mL | |
| 50 mM | 0.0405 mL | 0.2025 mL | 0.4051 mL | 1.0127 mL | |
| 60 mM | 0.0338 mL | 0.1688 mL | 0.3376 mL | 0.8439 mL | |
| 80 mM | 0.0253 mL | 0.1266 mL | 0.2532 mL | 0.6329 mL | |
| 100 mM | 0.0203 mL | 0.1013 mL | 0.2025 mL | 0.5063 mL |
Tiamulin Related Classifications
HY-B2060 Related Classifications
Powered by Bioz