Uralione E
Uralione E is a naturally occurring polycyclic polyprenylated acylphloroglucinol (PPAP). Uralione E exhibits a protective effect against corticosterone (HY-B1618)-induced damage in PC12 cells. In a free fatty acid (FFA)-induced L02 cell model, Uralione E dose-dependently reduces intracellular lipid accumulation, downregulates the expression of FASN and CD36, and upregulates the expression of ACOX1 and PPARα. Uralione E can be used in studies related to neuronal cell damage, lipid metabolism, and non-alcoholic steatohepatitis.
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- No. CAS: 1927024-53-0
- Fòrmula: C38H50O5
- Peso molecular:586.80
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
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Actividad biológica
Descripciòn
In Vitro
Uralione E (0.1-10.0 μM; 48 h) protects PC12 cells from corticosterone-induced injury, with cell viability reaching 86.6% at 10.0 μM after 48 h of co-treatment[1].
Uralione E (compound 20) (5-10 μM; 48 h) reduces intracellular lipid accumulation in FFA-induced L02 cells in a dose-dependent manner at 5 μM and 10 μM over 48 h[2].
Uralione E (compound 20) regulates lipid metabolism-related gene expression in FFA-induced L02 cells, suppressing CD36 and FASN while upregulating PPARα and ACOX1[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:rat pheochromocytoma PC12 cells
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Concentration:0.1, 0.3, 1.0, 3.0, 10.0 μM (co-incubated with 200 μM Corticosterone)
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Incubation Time:48 h
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Result:Exerted significant protective effects against corticosterone-induced PC12 cell injury.
Showed cell viability of 82.9% at 0.1 μM.
Showed cell viability of 72.8% at 0.3 μM.
Showed cell viability of 68.1% at 1.0 μM.
Showed cell viability of 78.0% at 3.0 μM.
Showed cell viability of 86.6% at 10.0 μM.
Increased cell viability substantially compared to the injured model group which had 55.6% viability.
Chemical Information
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No. CAS 1927024-53-0
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Peso molecular 586.80
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Fòrmula C38H50O5
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SMILES
O=C(C1=CC=CC=C1)[C@@]23[C@@](C)([C@H](C[C@]4(C(O[C@H](C4)C(C)(O)C)=C(C3=O)C/C=C(C)\C)C2=O)C/C=C(C)\C)CC/C=C(C)/C
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocolo
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3T3-L1 preadipocyte-to-adipocyte differentiation
3T3-L1 preadipocytes are induced to differentiate after growth arrest using adipogenic media containing insulin, dexamethasone, and IBMX; differentiation is assessed by lipid-droplet accumulation, triglyceride increase, Oil Red O staining, and adipocyte-marker induction such as PPARγ and C/EBPα.
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Lipid Droplets: Oil Red O/Sudan Dye Lipid Staining
Lipid droplets are intracellular organelles with a neutral-lipid core that stores triacylglycerols and sterol esters, and Oil Red O or Sudan dyes detect these hydrophobic lipid deposits by partitioning into retained lipids in fresh or frozen specimens. Oil Red O stains neutral triglycerides and lipids in frozen tissue sections or air-dried cytologic preparations, while Sudan Black B has also been used as a histochemical fat stain for lipid-rich tissue structures.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Uralione E Related Classifications
HY-N21699 Related Classifications
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