Isocannflavin B
Based on 1 Customer Validation
Isocannflavin B (Caflanone) is an autophagy inducer that induces strong autophagic intracytoplasmic vacuolization in breast cancer cells. Isocannflavin B inhibits ACE2-mediated viral entry as well as SARS-CoV-2 PLpro and 3CLpro, and inhibits HCoV-OC43 in vitro. Isocannflavin B reduces Akt phosphorylation and p21Cip1 expression in breast cancer cells. Isocannflavin B induces tumor cell apoptosis, autophagy, and cell cycle arrest, and inhibits cell proliferation. Isocannflavin B binds to the extracellular Ig-like domain of CSF1-R as a targeting ligand. Isocannflavin B can be used for research on breast cancer, viral infection, and Parkinson's disease.
For research use only. We do not sell to patients.
- CAS No.: 199167-23-2
- Formula: C21H20O6
- Molecular Weight:368.38
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
|
CSF1R |
ACE2 |
Akt |
p21 |
In Vitro
Isocannflavin B (25 μM; 24 h) induces autophagic cytoplasmic vacuolation in human breast cancer T47-D cells without causing chromatin condensation or nuclear fragmentation[1].
Isocannflavin B (10-100 μM; 0-4 h) reduces the phosphorylation level of Akt in human breast cancer T47-D cells, but has no effect on the phosphorylation of Akt or Erk 1/2 in human breast cancer MDA-MB-231 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:T47-D human breast cancer cells
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Concentration:1 μM, 10 μM, 25 μM, 50 μM, 100 μM
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Incubation Time:24 h
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Result:Caused cell growth arrest starting at 1 μM, with toxic effects evident from 25 μM.
Inhibited proliferation progressively stronger with increasing concentration.
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Cell Line:T47-D human breast cancer cells
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Concentration:25 μM, 100 μM
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Incubation Time:24 h
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Result:Caused a cell cycle distribution shift at 25 μM, with 41% of cells in G0/G1, 11% in S, 48% in G2/M, and 2% in apoptosis.
Induced G0/G1 cell-cycle arrest at 100 μM, with 60% of cells in G0/G1, 12% in S, 28% in G2/M, and 0% in apoptosis.
Showed no induction of apoptotic cell death at either concentration.
- RNA extraction experimental
- Somatic Cell Culture
- Breast Cancer Modeling
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Flow cytometric DNA-content cell-cycle staining
- Autophagy
- Apoptosis Solutions
- Lysosome and acidic-vesicle live-cell staining
- MTT Cell Proliferation Assay
- Macroautophagy Solutions
- Western Blot
- Research Protocol for Infectious Diseases
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- Protocol for Cell Cycle
- CCK-8/WST-8 Cell Proliferation Assay
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- Kinase activity and phosphorylation assays
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- Human pluripotent stem cell midbrain dopaminergic neuron differentiation
- Protocol for Kinase activity and phosphorylation assays
Chemical Information
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CAS No. 199167-23-2
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Appearance Solid
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Molecular Weight 368.38
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Formula C21H20O6
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Color White to yellow
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SMILES
O=C1C=C(C2=CC(OC)=C(O)C=C2)OC3=C(C/C=C(C)\C)C(O)=CC(O)=C31
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Synonyms
Caflanone; FBL-03G
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 7.37 mg/mL (20.01 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Brunelli E, et al. Flavonoid-induced autophagy in hormone sensitive breast cancer cells. Fitoterapia. 2009;80(6):327-332. [Content Brief]
[2]. Lowe H, et al. Non-Cannabinoid Metabolites of Cannabis sativa L. with Therapeutic Potential. Plants (Basel). 2021 Feb 20;10(2):400. [Content Brief]
[4]. Sun X, et al. Microglia-Specific Molecular Magnetic Resonance Imaging Probe Enables Noninvasive Separation of Parkinsonian Mice from Controls. bioRxiv [Preprint]. 2026 Jun 26:2026.06.23.734093. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.7146 mL | 13.5729 mL | 27.1459 mL | 67.8647 mL |
| 5 mM | 0.5429 mL | 2.7146 mL | 5.4292 mL | 13.5729 mL | |
| 10 mM | 0.2715 mL | 1.3573 mL | 2.7146 mL | 6.7865 mL | |
| 15 mM | 0.1810 mL | 0.9049 mL | 1.8097 mL | 4.5243 mL | |
| 20 mM | 0.1357 mL | 0.6786 mL | 1.3573 mL | 3.3932 mL |
Isocannflavin B Related Classifications
HY-N12677 Related Classifications
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Keywords
- Isocannflavin B
- 199167-23-2
- Caflanone
- FBL-03G
- SARS-CoV
- Apoptosis
- Autophagy
- Akt
- c-Fms
- SARS coronavirus
- PKB
- Protein kinase B
- CSF-1 receptor
- colony stimulating factor 1 receptor
- CSF-1R
- CSF1R
- 3CLpro
- ACE2-mediated viral entry
- Akt phosphorylation
- CSF1-R
- HCoV-OC43
- SARS-CoV-2 PLpro
- antiviral agent
- autophagy inducer
- breast cancer
- intracytoplasmic vacuolization
- Inhibitor
- inhibitor
- inhibit