2. Anti-infection Apoptosis Autophagy PI3K/Akt/mTOR
  3. SARS-CoV Apoptosis Autophagy Akt
  4. Isocannflavin B

Isocannflavin B (Caflanone) is a flavonoid compound. Isocannflavin B exhibits anti-tumor and anti-viral activities. Isocannflavin B induces apoptosis, autophagy and cell cycle arrest in tumor cells, and inhibits cell proliferation. Isocannflavin B inhibits HCoV-OC43. Isocannflavin B can be used in research related to cancer and COVID-19.

For research use only. We do not sell to patients.

Isocannflavin B

Isocannflavin B Chemical Structure

CAS No. : 199167-23-2

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500 μg In-stock

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Isocannflavin B Related Antibodies

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Description

Isocannflavin B (Caflanone) is a flavonoid compound. Isocannflavin B exhibits anti-tumor and anti-viral activities. Isocannflavin B induces apoptosis, autophagy and cell cycle arrest in tumor cells, and inhibits cell proliferation. Isocannflavin B inhibits HCoV-OC43. Isocannflavin B can be used in research related to cancer and COVID-19[1][2][3].

In Vitro

Isocannflavin B (1-100 μM; 24 h) inhibits the proliferation of human breast cancer T47-D cells in a concentration-dependent manner, with significant growth arrest observed starting at 1 μM, and a strong inhibitory effect at 100 μM[1].
Isocannflavin B (1-100 μM; 24 h) exerts no significant effect on the proliferation of MDA-MB-231 human breast cancer cells at concentrations ranging from 1 μM to 100 μM[1].
Isocannflavin B (25 μM; 24 h) induces autophagic cytoplasmic vacuolization in human breast cancer T47-D cells without causing chromatin condensation or nuclear fragmentation[1].
Isocannflavin B (25-100 μM; 24 h) induces cell cycle arrest in human breast cancer T47-D cells, and does not induce cell apoptosis at concentrations of 25 μM or 100 μM[1].
Isocannflavin B (10-100 μM; 0-4 h) reduces the phosphorylation level of Akt in human breast cancer T47-D cells, but exerts no effect on the phosphorylation of Akt or Erk 1/2 in human breast cancer MDA-MB-231 cells[1].
Isocannflavin B potently inhibits human coronavirus OC43 in vitro, with an EC50 of 0.42 µM[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Isocannflavin B Related Antibodies

Cell Proliferation Assay[1]

Cell Line: T47-D human breast cancer cells
Concentration: 1 μM, 10 μM, 25 μM, 50 μM, 100 μM
Incubation Time: 24 h
Result: Caused cell growth arrest starting at 1 μM, with toxic effects evident from 25 μM.
Inhibited proliferation progressively stronger with increasing concentration.

Cell Cycle Analysis[1]

Cell Line: T47-D human breast cancer cells
Concentration: 25 μM, 100 μM
Incubation Time: 24 h
Result: Caused a cell cycle distribution shift at 25 μM, with 41% of cells in G0/G1, 11% in S, 48% in G2/M, and 2% in apoptosis.
Induced G0/G1 cell-cycle arrest at 100 μM, with 60% of cells in G0/G1, 12% in S, 28% in G2/M, and 0% in apoptosis.
Showed no induction of apoptotic cell death at either concentration.
In Vivo

Isocannflavin B enhances survival in preclinical mouse models of metastatic pancreatic cancer by promoting tumor cell apoptosis[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

368.38

Formula

C21H20O6
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O=C1C=C(C2=CC(OC)=C(O)C=C2)OC3=C(C/C=C(C)\C)C(O)=CC(O)=C31
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 7.37 mg/mL (20.01 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7146 mL 13.5729 mL 27.1459 mL
5 mM 0.5429 mL 2.7146 mL 5.4292 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7146 mL 13.5729 mL 27.1459 mL 67.8647 mL
5 mM 0.5429 mL 2.7146 mL 5.4292 mL 13.5729 mL
10 mM 0.2715 mL 1.3573 mL 2.7146 mL 6.7865 mL
15 mM 0.1810 mL 0.9049 mL 1.8097 mL 4.5243 mL
20 mM 0.1357 mL 0.6786 mL 1.3573 mL 3.3932 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Isocannflavin B199167-23-2Caflanone FBL-03GSARS-CoVApoptosisAutophagyAktSARS coronavirusPKBProtein kinase BMDA-MB-231 human breast cancer cellsPLproautophagyapoptosis3CLproACE2 receptorHCoV-OC43metastatic pancreatic cancerT47-D human breast cancer cellsInhibitorinhibitorinhibit

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