製品番号: HY-17024

シクロパミン

(別名: Cyclopamine; 11-Deoxojervine)

56 Cited Publications

カスタマーレビュー

Based on 56 publication(s) in Google Scholar

Cyclopamine is a Hedgehog (Hh) pathway antagonist with an IC₅₀ of 46 nM in the Hh cell assay. Cyclopamine is also a selective Smo inhibitor.

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純度: 99.68%
CAS 番号: 4449-51-8
分子式: C₂₇H₄₁NO₂
分子量:411.62

保管条件:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Cyclopamine 構造式

CAS 番号: 4449-51-8

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価格（税別）

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数量

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Fluorescent Dye

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生物活性
- 製品説明
- IC₅₀ & Target
- Cellular Effect
- 体外実験
- 体内実験
化学情報
Publications (56)
溶剤 & 溶解度
- 体外
- 体内
プロトコル
純度とドキュメンテーション
参考文献
Help & FAQs
関連分類
Bioz

生物活性

製品説明

Cyclopamine is a Hedgehog (Hh) pathway antagonist with an IC₅₀ of 46 nM in the Hh cell assay. Cyclopamine is also a selective Smo inhibitor.

IC₅₀ & Target

Human Endogenous Metabolite

Endogenous Metabolite アイソフォーム固有の製品をすべて表示 :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	49 μM Compound: 1	Anticancer activity against human A549 cells by MTS assay Anticancer activity against human A549 cells by MTS assay	[PMID: 18221872]
C3H 10T1/2	EC₅₀	0.17 μM Compound: 1	Inhibition of Hedgehog pathway-dependent oxysterol-induced mouse C3 H10T1/2 cell differentiation assessed as alkaline phosphatase production after 72 hrs Inhibition of Hedgehog pathway-dependent oxysterol-induced mouse C3 H10T1/2 cell differentiation assessed as alkaline phosphatase production after 72 hrs	[PMID: 18842035]
C3H 10T1/2	IC₅₀	0.6 μM Compound: Cyclopamine	Inhibition of N-terminal SHH activated pathway in mouse C3H10T1/2 cells assessed as SAG-induced cell differentiation by alkaline phosphatase assay Inhibition of N-terminal SHH activated pathway in mouse C3H10T1/2 cells assessed as SAG-induced cell differentiation by alkaline phosphatase assay	[PMID: 21592788]
C3H 10T1/2	IC₅₀	0.6 μM Compound: cyclopamine	Inhibition of human Shh-induced mouse C3H10T1/2 cell differentiation after 48 hrs by alkaline phosphatase reporter assay Inhibition of human Shh-induced mouse C3H10T1/2 cell differentiation after 48 hrs by alkaline phosphatase reporter assay	[PMID: 23074541]
C3H 10T1/2	IC₅₀	0.62 μM Compound: Cyclopamine	Inhibition of SAG-induced differentiation of mouse mesenchymal pluripotent C3H10T1/2 cells to alkaline phosphatase positive oeseoblasts after 6 hrs Inhibition of SAG-induced differentiation of mouse mesenchymal pluripotent C3H10T1/2 cells to alkaline phosphatase positive oeseoblasts after 6 hrs	[PMID: 22268551]
CHO	IC₅₀	1200 nM Compound: cyclopamine	Antagonist activity at mouse Smo receptor expressed in CHO cells by [3H]Hh-Ag binding assay Antagonist activity at mouse Smo receptor expressed in CHO cells by [3H]Hh-Ag binding assay	[PMID: 19091559]
CHO	IC₅₀	280 nM Compound: cyclopamine	Antagonist activity at human Smo receptor expressed in CHO cells by [3H]Hh-Ag binding assay Antagonist activity at human Smo receptor expressed in CHO cells by [3H]Hh-Ag binding assay	[PMID: 19091559]
Daoy	IC₅₀	0.16 μM Compound: 2, Cyc	Inhibition of Hedgehog signaling in human DaOY cells assessed as downregulation of Gli1 mRNA expression after 48 hrs by RT-PCR analysis Inhibition of Hedgehog signaling in human DaOY cells assessed as downregulation of Gli1 mRNA expression after 48 hrs by RT-PCR analysis	[PMID: 24900716]
HEK293	IC₅₀	64 nM Compound: Cyclopamine	Displacement of BODIPY-labelled cyclopamine from human Smo receptor expressed in HEK293 cells after 2 hrs by fluorescence microscopy Displacement of BODIPY-labelled cyclopamine from human Smo receptor expressed in HEK293 cells after 2 hrs by fluorescence microscopy	[PMID: 22268551]
Medulloblastoma cell	EC₅₀	1 μM Compound: 16	Antiproliferative activity against mouse medulloblastoma cells harboring heterozygous ptch1 gene by MTT assay Antiproliferative activity against mouse medulloblastoma cells harboring heterozygous ptch1 gene by MTT assay	[PMID: 17417631]
MEF	IC₅₀	1.5 μM Compound: cyclopamine	Inhibition of Smo in mouse Ptch-deficient MEF cells assessed as inhibition of Shh-induced Gli1 transcriptional activity Inhibition of Smo in mouse Ptch-deficient MEF cells assessed as inhibition of Shh-induced Gli1 transcriptional activity	[PMID: 23074541]
MEF	IC₅₀	1.9 μM Compound: cyclopamine	Inhibition of Smo in mouse Ptch-deficient MEF cells assessed as inhibition of Shh-induced Gli-responsive betagalactosidase activity after 30 hrs by BetaGLo assay Inhibition of Smo in mouse Ptch-deficient MEF cells assessed as inhibition of Shh-induced Gli-responsive betagalactosidase activity after 30 hrs by BetaGLo assay	[PMID: 23074541]
Shh Light II	EC₅₀	500 nM Compound: 3	Inhibition of SHH in mouse Shh Light2 cells by GLI-responsive firefly luciferase reporter gene assay Inhibition of SHH in mouse Shh Light2 cells by GLI-responsive firefly luciferase reporter gene assay	[PMID: 19309080]
Shh Light II	IC₅₀	0.3 μM Compound: Cyclopamine	Inhibition of SHH pathway in mouse Shh Light2 cells after 40 hrs by Gli-dependent luciferase reporter gene assay Inhibition of SHH pathway in mouse Shh Light2 cells after 40 hrs by Gli-dependent luciferase reporter gene assay	[PMID: 21592788]
Shh Light II	IC₅₀	0.3 μM Compound: Cyclopamine	Inhibition of Smo-mediated Hh signaling in human Shh-light2 cells by luciferase reporter gene assay Inhibition of Smo-mediated Hh signaling in human Shh-light2 cells by luciferase reporter gene assay	[PMID: 22268551]
Shh Light II	IC₅₀	1312 nM Compound: Cyclopamine	Inhibition of SHH in mouse Shh-Light2 cells after 48 hrs by Gli1 reporter gene assay in presence of SAG Inhibition of SHH in mouse Shh-Light2 cells after 48 hrs by Gli1 reporter gene assay in presence of SAG	[PMID: 19541490]
Shh Light II	IC₅₀	484 nM Compound: Cyc	Antagonist activity at Smo in mouse Shh-Light 2 cells assessed as inhibition of Shh-induced Gli1-reporter activity after 2 days by dual-luciferase reporter gene method Antagonist activity at Smo in mouse Shh-Light 2 cells assessed as inhibition of Shh-induced Gli1-reporter activity after 2 days by dual-luciferase reporter gene method	[PMID: 23063522]
TM3	IC₅₀	46 nM Compound: cyclopamine	Inhibition of Hedgehog signaling pathway in mouse TM3 cells bearing pTA-8xGli-Luc reporter construct assessed as transcriptional modulation of Gli after 48 hrs by luciferase assay Inhibition of Hedgehog signaling pathway in mouse TM3 cells bearing pTA-8xGli-Luc reporter construct assessed as transcriptional modulation of Gli after 48 hrs by luciferase assay	[PMID: 19091559]
U-87MG ATCC	IC₅₀	15.5 μM Compound: Cyclopamine	Cytotoxicity against human U87 cells assessed as viability in presence of beta glucuronidase Cytotoxicity against human U87 cells assessed as viability in presence of beta glucuronidase	[PMID: 20116904]
U-87MG ATCC	IC₅₀	22.5 μM Compound: Cyc	Antiproliferative activity against human U87MG cells after 72 hrs by MTS assay Antiproliferative activity against human U87MG cells after 72 hrs by MTS assay	[PMID: 22226657]

体外実験

Treatment with small molecule Hh inhibitors such as HhAntag and the natural product Cyclopamine, both binding to Smo, induces tumor remission in a genetic mouse model of medulloblastoma^[1]. Cyclopamine is a Hedgehog (Hh) pathway antagonist. Cyclopamine suppresses cell growth. Cyclopamine (3 μM) suppression of Hh pathway activity and growth in digestive tract tumour cell lines correlates with expression of PTCHmRNA^[2]. Cyclopamine is a steroidal alkaloid that inhibits Hh signalling through direct interaction with Smo^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

Cyclopamine causes durable regression of xenograft tumors. Tumors in Cyclopamine-treated animals, regress completely by 12 days^[2]. Cyclopamine (1.2 mg) treatment blocks tumour formation of human pancreatic adenocarcinoma cells after transplantation into nude mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

化学情報

CAS 番号 4449-51-8
性状 Solid
分子量 411.62
分子式 C₂₇H₄₁NO₂
Color White to off-white
SMILES

CC1=C2[C@@]([C@@]3([H])[C@]([C@@]4(C(C[C@@H](O)CC4)=CC3)C)([H])C2)([H])CC[C@@]15[C@@H]([C@@]6([H])[C@](C[C@H](C)CN6)([H])O5)C
別名

Cyclopamine; 11-Deoxojervine
Structure Classification
- Alkaloids
- Pyridine Alkaloids
- Steroids
Initial Source
- Endogenous metabolite
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (56)

All WBIF

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Journal Impact Factor

Nature

Pre-assembly of biomolecular condensate seeds drives RSV replication. [Abstract]

2026 Apr;652(8108):189-200. PMID: 41606345
Cell Mol Immunol

Chaperone- and PTM-mediated activation of IRF1 tames radiation-induced cell death and the inflammatory response. [Abstract]

2024 Aug;21(8):856-872. PMID: 38849539
Nat Commun

Streamlined metal-based hydrogel facilitates stem cell differentiation, extracellular matrix homeostasis and cartilage repair in male rats. [Abstract]

2025 May 10;16(1):4344. PMID: 40346121
Nat Commun

The HHIP-AS1 lncRNA promotes tumorigenicity through stabilization of dynein complex 1 in human SHH-driven tumors. [Abstract]

2022 Jul 13;13(1):4061. PMID: 35831316
Cell Death Differ

Constitutive GLI1 expression in chondrosarcoma is regulated by major vault protein via mTOR/S6K1 signaling cascade. [Abstract]

2021 Jul;28(7):2221-2237. PMID: 33637972
Cell Death Dis

HHIPL2 positively governs Hedgehog signaling to accelerate non-small cell lung cancer progression via enhancing HNRNPC-mediated HNF1A mRNA stabilization. [Abstract]

2025 Dec 18. PMID: 41413056
Pharmacol Res

Restoration of early deficiency of axonal guidance signaling by guanxinning injection as a novel therapeutic option for acute ischemic stroke. [Abstract]

2021 Mar:165:105460. PMID: 33513357
Cell Death Dis

Vascular endothelial growth factor 165 inhibits pro-fibrotic differentiation of stromal cells via the DLL4/Notch4/smad7 pathway. [Abstract]

2019 Sep 12;10(9):681. PMID: 31515487

Cyclopamine purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Sep 12;10(9):681. [Abstract]

Endometrial stromal cells are stimulated with 10 ng/mL TGFβ1 for 48 h. Then these cells are treated with 10 μM Cyc for 24 h and/or cocultured with 10 ng/mL VEGF165 for another 12 or 24 h. The protein levels of collagen 1, α-SMA and smad7 are examined.

Cyclopamine purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Sep 12;10(9):681. [Abstract]

Endometrial stromal cells are stimulated with 10 ng/mL TGFβ1 for 48 h. Then these cells are treated with 10 μM Cyc for 24 h and/or cocultured with 10 ng/mL VEGF165 for another 12 or 24 h. The protein levels of collagen 1, α-SMA and smad7 are examined.
J Hazard Mater

Advanced understanding of the neurotoxicity of brominated flame retardants: Integrating human neural organoids with multi-omics. [Abstract]

2025 Dec 5:500:140529. PMID: 41297252
Cell Death Discov

Smoothened inhibition leads to decreased cell proliferation and suppressed tissue fibrosis in the development of benign prostatic hyperplasia. [Abstract]

2021 May 18;7(1):115. PMID: 34006832
J Orthop Translat

RPL35 downregulated by mechanical overloading promotes chondrocyte senescence and osteoarthritis development via Hedgehog-Gli1 signaling. [Abstract]

2024 Apr 1:45:226-235. PMID: 38596341
Chin Med J (Engl)

Baicalin attenuates dexamethasone-induced apoptosis of bone marrow mesenchymal stem cells by activating the hedgehog signaling pathway. [Abstract]

2023 Aug 5;136(15):1839-1847. PMID: 36804262
Int J Nanomedicine

Role of Hedgehog-Gli1 signaling in the enhanced proliferation and differentiation of MG63 cells enabled by hierarchical micro-/nanotextured topography. [Abstract]

2017 Apr 20:12:3267-3280. PMID: 28458545

Cyclopamine purchased from MedChemExpress. Usage Cited in: Int J Nanomedicine. 2017 Apr 20:12:3267-3280. [Abstract]

The Smo inhibitor Cyclopamine suppresses the activity of Hedgehog–Gli1 signaling. The gene expression and protein production of Smo in MG63 osteoblasts after 7 days of incubation on the different titanium surfaces.
Neural Regen Res

M2 macrophages mediate fibrotic scar formation in the early stages after cerebral ischemia in rats. [Abstract]

2023 Oct;18(10):2208-2218. PMID: 37056140
J Genet Genomics

Reduced Smoothened level rescues Aβ-induced memory deficits and neuronal inflammation in animal models of Alzheimer's disease. [Abstract]

2018 May 20;45(5):237-246. PMID: 29807798
Cell Biosci

Increased pulmonary blood flow leads to alveolar dysplasia during the early postnatal developmental stage. [Abstract]

2025 Nov 24;15(1):161. PMID: 41287066
Biochem Pharmacol

Hydroquinone-selected chronic myelogenous leukemia cells are sensitive to chloroquine-induced cytotoxicity via MCL1 suppression and glycolysis inhibition. [Abstract]

2023 Dec:218:115934. PMID: 37989415
Biochem Pharmacol

Identification of pseudolaric acid B as a novel Hedgehog pathway inhibitor in medulloblastoma. [Abstract]

2021 Aug:190:114593. PMID: 33964282
Life Sci

Knockdown of HIF-1α inhibits the proliferation and migration of outer root sheath cells exposed to hypoxia in vitro: An involvement of Shh pathway. [Abstract]

2017 Dec 15:191:82-89. PMID: 29030089

Cyclopamine purchased from MedChemExpress. Usage Cited in: Life Sci. 2017 Dec 15:191:82-89. [Abstract]

ORS cells are exposed to 3% oxygen for 48 h in presence or absence of Shh pathway inhibitor cyclopamine (5 μM) or GANT61 (10 μM). Immunofluorescence assay using anti-PCNA antibody is performed to detect the proliferative ORS cells (red). Cell nuclei are counterstained with DAPI (blue).
Int Immunopharmacol

Tubular STING drives renal fibrosis via extracellular vesicle-mediated activation of the SMO/GLI-1 pathway. [Abstract]

2026 Apr 1:174:116350. PMID: 41679184
Int J Mol Sci

O-GlcNAcylation in Gli1+ Mesenchymal Stem Cells Is Indispensable for Bone Formation and Fracture Healing. [Abstract]

2025 Mar 18;26(6):2712. PMID: 40141354
Int Immunopharmacol

Cyclopamine inhibits corneal neovascularization and fibrosis by alleviating inflammatory macrophage recruitment and endothelial cell activation. [Abstract]

2025 Jan 11:147:114025. PMID: 39799735
Int Immunopharmacol

Interleukin 25 promotes muscle regeneration in sarcopenia by regulating macrophage-mediated Sonic Hedgehog signaling. [Abstract]

2024 Jul 21:139:112662. PMID: 39038385
Int J Mol Sci

Nile Tilapia (Oreochromis niloticus) Patched1 Mutations Disrupt Cardiovascular Development and Vascular Integrity through Smoothened Signaling. [Abstract]

2024 Mar 15;25(6):3321. PMID: 38542295
Front Pharmacol

Epigallocatechin-3-Gallate Promotes the Growth of Mink Hair Follicles Through Sonic Hedgehog and Protein Kinase B Signaling Pathways. [Abstract]

2018 Jun 26:9:674. PMID: 29997505

Cyclopamine purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2018 Jun 26:9:674. [Abstract]

Cyclopamine and GANT61 decrease the level of Gli1 as evidenced by western blot.
J Ovarian Res

GIMAP7 induces oxidative stress and apoptosis of ovarian granulosa cells in polycystic ovary syndrome by inhibiting sonic hedgehog signalling pathway. [Abstract]

2022 Dec 30;15(1):141. PMID: 36581994
Biochim Biophys Acta Mol Basis Dis

YAP/Aurora A-mediated ciliogenesis regulates ionizing radiation-induced senescence via Hedgehog pathway in tumor cells. [Abstract]

2024 Feb 10;1870(4):167062. PMID: 38342416
Sci Rep

SLC25A39 regulates Hedgehog signaling to promote tumor progression and sorafenib resistance in hepatocellular carcinoma. [Abstract]

2025 Oct 15;15(1):36061. PMID: 41093994
J Cell Mol Med

Procyanidin B2 alleviates liver injury caused by cold stimulation through Sonic hedgehog signalling and autophagy. [Abstract]

2021 Aug;25(16):8015-8027. PMID: 34155807
J Cell Mol Med

CRKL is a critical target of Hh-GLI2 pathway in lung adenocarcinoma. [Abstract]

2021 Jul;25(13):6280-6288. PMID: 34076957
iScience

Evolution and Developmental Diversity of Skin Spines in Pufferfishes. [Abstract]

2019 Sep 27;19:1248-1259. PMID: 31353167
Neurochem Res

Sonic Hedgehog Promotes Neurite Outgrowth of Primary Cortical Neurons Through Up-Regulating BDNF Expression. [Abstract]

2016 Apr;41(4):687-95. PMID: 26459360

Cyclopamine purchased from MedChemExpress. Usage Cited in: Neurochem Res. 2016 Apr;41(4):687-95. [Abstract]

Western blot and RT-qPCR assays of BDNF protein and gene expressions in primary cortical neurons in different experimental conditions. Immunoblotting bands of BDNF protein.
FASEB J

Loss of sonic hedgehog gene leads to muscle development disorder and megaesophagus in mice. [Abstract]

2018 Oct;32(10):5703-5715. PMID: 29768039
Development

A cell-autonomous role for primary cilium-mediated signaling in long-range commissural axon guidance. [Abstract]

2024 Aug 19:dev.202788. PMID: 39157903
J Cell Sci

The non-canonical Smoothened-AMPK axis regulates Smaug1 biomolecular condensates. [Abstract]

2025 Feb 10:jcs.263433. PMID: 39925132
J Funct Foods

2024 Jan, 112, 105960.
Exp Biol Med

Pseudomonas aeruginosa-mannose-sensitive hemagglutinin inhibits chemical-induced skin cancer through suppressing hedgehog signaling. [Abstract]

2020 Feb;245(3):213-220. PMID: 31903775
Cell Biol Int

GATA3-NLRP1 Axis Inhibits Hepatocellular Carcinoma Progression Through the Hedgehog Signaling. [Abstract]

2026 Jan;50(1):e70116. PMID: 41424411
Exp Cell Res

Dl-3-n-butylphthalide promotes neurite outgrowth of primary cortical neurons by Sonic Hedgehog signaling via upregulating Gap43. [Abstract]

2021 Jan 15;398(2):112420. PMID: 33296663
Mol Biol Rep

Ectoderm mesenchymal stem cells promote osteogenic differentiation of MC3T3-E1 cells by targeting sonic hedgehog signaling pathway. [Abstract]

2023 Feb;50(2):1293-1302. PMID: 36456772
Tissue Cell

SURF4 inhibits autophagy to enhance lung adenocarcinoma progression and stemness through Hedgehog pathway regulation. [Abstract]

2026 Jan 26:100:103351. PMID: 41637921
J Appl Toxicol

Circ_0027470 promotes cadmium exposure-induced prostatic fibrosis via sponging miRNA-1236-3p and stimulating SHH signaling pathway. [Abstract]

2023 Jul;43(7):973-981. PMID: 36617218
Med Princ Pract

IGFBP5 promotes neuronal apoptosis in a 6-OHDA-toxicant model of Parkinson's disease by inhibiting the sonic hedgehog signalling pathway. [Abstract]

2024;33(3):269-280. PMID: 38565090
Biochem Biophys Res Commun

PAX6 contributes to the activation and proliferation of hepatic stellate cells via activating Hedgehog/GLI1 pathway. [Abstract]

2020 May 28;526(2):314-320. PMID: 32209258
Hematology

SPRY1 promotes cell proliferation and inhibits apoptosis by activating Hedgehog pathway in acute myeloid leukemia. [Abstract]

2022 Dec;27(1):1-10. PMID: 34957932
Connect Tissue Res

Overexpression of laminin α4 facilitates proliferation and migration of fibroblasts in knee arthrofibrosis by targeting canonical Shh/Gli1 signaling. [Abstract]

2020 Jun 10;1-11. PMID: 32449381
Br Poult Sci

Characterisation of Shh gene expression in the skin and feather follicles of Jilin white geese during early embryonic development. [Abstract]

2026 Apr 13:1-9. PMID: 41972330
Cancer Biother Radiopharm

A Noncanonical Hedgehog Signaling Exerts a Tumor-Promoting Effect on Pancreatic Cancer Cells Via Induction of Osteopontin Expression. [Abstract]

2021 Dec 31. PMID: 34978897
Mol Cell Toxicol

2025 Oct 23.
Biomed Pharmacother

Drug target proteome profiling identifies HES1-driven mitotic catastrophe in ovarian serous carcinoma. [Abstract]

2025 Nov 6:193:118716. PMID: 41202420
Reprod Breed

2025 Sep.
bioRxiv

The molecular chronology of mammary epithelial cell fate switching. [Abstract]

2024 Nov 4:2024.10.08.617155. PMID: 39415993
Biomed Pharmacother

Salidroside promotes angiogenesis after cerebral ischemia in mice through Shh signaling pathway. [Abstract]

2024 May:174:116625. PMID: 38643543
Research Square Preprint

2023 Oct 17.
Research Square Print

2022 May.
Oxid Med Cell Longev

Mitochondrial Protection and Against Glutamate Neurotoxicity via Shh/Ptch1 Signaling Pathway to Ameliorate Cognitive Dysfunction by Kaixin San in Multi-Infarct Dementia Rats. [Abstract]

2021 Jul 9:2021:5590745. PMID: 34306310

溶剤 & 溶解度

体外:

DMSO : 5 mg/mL (12.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 5 mg/mL (12.15 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4294 mL

12.1471 mL

24.2943 mL

5 mM

0.4859 mL

2.4294 mL

4.8589 mL

10 mM

0.2429 mL

1.2147 mL

2.4294 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 0.5 mg/mL (1.21 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 0.5 mg/mL (1.21 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 0.5 mg/mL (1.21 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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g
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

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The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

プロトコル

Cell Assay^[2]

Cells are cultured in triplicate in 96-well plates in assay media to which 5E1 monoclonal antibody, ShhNp and/or Cyclopamine (3 μM) are added at 0 h at concentrations indicated in the main text. Viable cell mass is determined by optical density measurements at 490 nm (OD₄₉₀) at 2 and 4 days using the CellTiter96 colorimetric assay. Relative growth is calculated as OD (day 4)-OD (day 2)/OD (day 2)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[3]

Mice^[3]
A total of 0.1 mL Hanks’ balanced salt solution and matrigel (1:1) containing 2×10⁶ cells is injected subcutaneously into CD-1 nude mice. Tumors are grown for 4 days to a minimum volume of 125 mm³; treatment is initiated simultaneously for all subjects. Mice are injected subcutaneously with vector alone (triolein:ethanol 4:1 v/v) or a Cyclopamine suspension (1.2 mg per mouse in triolein:ethanol 4:1 v/v) daily for 7 days. At the end of the treatment period, tumours are excised from mice, weighed and then fixed for 3 h at 4°C with 4% paraformaldehyde, embedded in paraffin wax and sectioned (6 µm). Apoptotic cells are identified by TUNEL using recombinant Tdt. Sections are then counterstained with eosin. Eight ×20-magnified fields from regions corresponding to the exterior, middle and interior of two control and two cyclopamine-treated tumours are chosen at random. We counted the number of TUNEL-positive nuclei manually. Haematoxylin/eosin staining is done.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

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COA (2659 KB)
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参考文献

[1]. Peukert S, et al. Identification and structure-activity relationships of ortho-biphenyl carboxamides as potent Smoothened antagonists inhibiting the Hedgehog signaling pathway. Bioorg Med Chem Lett, 2009, 19(2), 328-331. [Content Brief]

[2]. Berman DM, et al. Widespread requirement for Hedgehog ligand stimulation in growth of digestive tract tumours. Nature, 2003, 425(6960), 846-851. [Content Brief]

[3]. Thayer SP, et al. Hedgehog is an early and late mediator of pancreatic cancer tumorigenesis. Nature, 2003, 425(6960), 851-856. [Content Brief]

[4]. Ma W, et al. Reduced Smoothened level rescued Aβ-induced memory deficits and neuronal inflammation in animal models of Alzheimer's disease. J Genet Genomics. 2018 May 20;45(5):237-246. [Content Brief]

[5]. Qi Wan, et al. Overexpression of Laminin α4 Facilitates Proliferation and Migration of Fibroblasts in Knee Arthrofibrosis by Targeting Canonical Shh/Gli1 Signaling. Connect Tissue Res. 2020 May 24. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / Ethanol	1 mM	2.4294 mL	12.1471 mL	24.2943 mL	60.7356 mL
	5 mM	0.4859 mL	2.4294 mL	4.8589 mL	12.1471 mL
	10 mM	0.2429 mL	1.2147 mL	2.4294 mL	6.0736 mL

Keywords

Cyclopamine

56 Cited Publications

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MedChemExpress（MCE）の使用を引用している文献 Cyclopamine

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Complete Stock Solution Preparation Table

Cyclopamine 関連分類

HY-17024 関連分類