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製品番号: HY-P10923

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KYS-1

Name: KYS-1
Brand: MedChemExpress
SKU: HY-P10923
Price: 130.00 USD
Availability: InStock
Rating: 5 (1 reviews)

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Based on 1 Customer Validation

KYS-1 is a specific fluorescent substrate for Cathepsin E, with a K_m of 1.90 μM. KYS-1 is specifically cleaved by Cathepsin E at the Phe-Leu bond under acidic conditions (optimal pH approximately 4.0), relieving fluorescence quenching and releasing a strong signal, thereby enabling measurement of Cathepsin E enzyme activity. KYS-1 can be used for breast cancer research.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

純度 : 99.34%
CAS 番号: 592544-81-5
分子式: C₆₁H₈₂N₁₆O₁₈
分子量:1327.40

保管条件:

Sealed storage, away from moisture.

Powder -80°C, 2 years , -20°C, 1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

KYS-1 構造式

CAS 番号: 592544-81-5

容量

価格（税別）

在庫状況

数量

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

IC₅₀ & Target

^[1]

Cathepsin E

1.90 μM (Km)

Cathepsin アイソフォーム固有の製品をすべて表示 :

体外実験

KYS-1 (200 µM; 10 min) is hydrolyzed by cathepsin E in wild-type mouse serum, where more than 75% of the KYS-1 hydrolytic activity can be immunoprecipitated by anti-rat CatE IgG^[2].
KYS-1 is hydrolyzed by purified recombinant cathepsin E at pH 5.0, and GlcN does not alter this activity^[3].
KYS-1 (6 h) can report CatE enzyme activity in MOG35-55 (HY-P1240)-stimulated mouse neutrophil whole-cell lysates^[4].
KYS-1 (10-40 μM; 10 min) is selectively hydrolyzed by cathepsin E at pH 4.0, with Km values of 1.90, 2.28, and 1.79 μM for human erythrocyte, rat erythrocyte, and rat stomach-derived cathepsin E, respectively, whereas cathepsin D, pepsin A, and cathepsins B, H, and L hydrolyze it to a lesser extent^[1].
KYS-1 (20 μM; 10 min) assay detects cathepsin E in the range of 0.25-4 ng and remains linear under standard conditions^[1].
KYS-1 confirms that cathepsin E activity is completely lost in mammary gland extracts from CatE-/- mice^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Breast Cancer Modeling

化学情報

CAS 番号 592544-81-5
性状 Solid
分子量 1327.40
分子式 C₆₁H₈₂N₁₆O₁₈
Color Light yellow to yellow
配列

{Mca}-Gly-Ser-Pro-Ala-Phe-Leu-Ala-{Lys(Dnp)}-{d-Arg}-NH2
シーケンスの短縮

{Mca}-GSPAFLA-{Lys(Dnp)}-{d-Arg}-NH2
輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (75.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

0.7534 mL

3.7668 mL

7.5335 mL

5 mM

0.1507 mL

0.7534 mL

1.5067 mL

10 mM

0.0753 mL

0.3767 mL

0.7534 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (284 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Yasuda Y, et al. A new selective substrate for cathepsin E based on the cleavage site sequence of alpha2-macroglobulin. Biological chemistry. 2005 Mar;386(3):299-305. [Content Brief]

[2]. Kawakubo T, et al. Repression of cathepsin E expression increases the risk of mammary carcinogenesis and links to poor prognosis in breast cancer. Carcinogenesis. 2014 Mar;35(3):714-26.

[3]. Božič J, et al. Glucosamine prevents polarization of cytotoxic granules in NK-92 cells by disturbing FOXO1/ERK/paxillin phosphorylation. PloS one. 2018;13(7):e0200757.

[4]. Harada Y, et al. Cathepsin E in neutrophils contributes to the generation of neuropathic pain in experimental autoimmune encephalomyelitis. Pain. 2019 Sep;160(9):2050-2062.

[5]. Goto S, et al. Role of cathepsin E in decidual macrophage of patients with recurrent miscarriage. Molecular human reproduction. 2014 May;20(5):454-62.

[6]. Kitamura K, et al. Development of systemic in vitro evolution and its application to generation of peptide-aptamer-based inhibitors of cathepsin E. Journal of molecular biology. 2009 Apr 17;387(5):1186-98.

[7]. Kawakubo T, et al. Gene expression profiling of mammary glands of cathepsin E-deficient mice compared with wild-type littermates. Biochimie. 2008 Feb;90(2):396-404.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	0.7534 mL	3.7668 mL	7.5335 mL	18.8338 mL
	5 mM	0.1507 mL	0.7534 mL	1.5067 mL	3.7668 mL
	10 mM	0.0753 mL	0.3767 mL	0.7534 mL	1.8834 mL
	15 mM	0.0502 mL	0.2511 mL	0.5022 mL	1.2556 mL
	20 mM	0.0377 mL	0.1883 mL	0.3767 mL	0.9417 mL
	25 mM	0.0301 mL	0.1507 mL	0.3013 mL	0.7534 mL
	30 mM	0.0251 mL	0.1256 mL	0.2511 mL	0.6278 mL
	40 mM	0.0188 mL	0.0942 mL	0.1883 mL	0.4708 mL
	50 mM	0.0151 mL	0.0753 mL	0.1507 mL	0.3767 mL
	60 mM	0.0126 mL	0.0628 mL	0.1256 mL	0.3139 mL

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MOQ

Minimum order quantity

100 mg

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生物活性
- 製品説明
- IC₅₀ & Target
- 体外実験
化学情報
溶剤 & 溶解度
- 体外実験
純度とドキュメンテーション
参考文献

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KYS-1

Cathepsin アイソフォーム固有の製品をすべて表示

生物活性

製品説明

IC50 & Target

体外実験

化学情報

溶剤 & 溶解度

体外:

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

KYS-1 関連分類

HY-P10923 関連分類

Bioz

Keywords

最近チェックした製品

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