MD13
Based on 1 Customer Validation
MD13 is a MIF PROTAC degrader with a DC50 of approximately 100 nM. MD13 induces ubiquitination and degradation of MIF by recruiting the Cereblon Cullin RING E3 ubiquitin ligase complex. MD13 inactivates the MAPK pathway and induces G2/M phase cell cycle arrest. MD13 exhibits antiproliferative effects in 3D tumor spheroid models. MD13 can be used in lung cancer-related research.
(Pink: Macrophage migration inhibitory factor (MIF) Target protein ligand; Blue: Cereblon ligand (HY-10984); Black: linker).
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.96%
- CAS 番号: 2758431-97-7
- 分子式: C35H35N5O8
- 分子量:653.68
-
保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
-
溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
-
Immunology/Inflammation Compound Library
HY-L007
-
Anti-Cancer Compound Library
HY-L025
-
Heterocyclic Compound Library
HY-L138
-
PROTAC Library
HY-L151
-
Macrophage Related Compound Library
HY-L174
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Bioactive Compound Library
HY-L001
-
High-Throughput Bioactive Compound Library
HY-L205
PROTACs アイソフォーム固有の製品をすべて表示
More
生物活性
|
Cereblon |
MD13 potently inhibits purified MIF tautomerase activity with a Ki of 71 nM[1].
MD13 (0.01-20 μM; 3-48 h) induces potent, time-dependent, cereblon- and proteasome-dependent MIF degradation in A549 cells, with a DC50 of ~100 nM and maximal degradation of 90-95%[1].
MD13 (0.01-20 μM; 72 h) dose-dependently inhibits 2D monolayer proliferation of A549 cells, achieving ~50% inhibition at 20 μM after 72 h[1].
MD13 (2 μM; 6-48 h) inhibits ERK phosphorylation in A549 cells, reducing pERK levels by ~50% at 2 μM after 24 h of treatment[1].
MD13 (1-5 μM; 48 h) dose-dependently induces G2/M phase cell cycle arrest in A549 cells, increasing the proportion of G2/M-phase cells to 23% at 5 μM after 48 h[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:A549 human lung adenocarcinoma cells
-
Concentration:0.01-20 μM (dose-response); 2 μM (time-course); 0.2-20 μM; 2 μM (with 2 μM Bortezomib co-treatment); 2 μM (with 50 μM MIF inhibitor 3 or 50 μM CRBN inhibitor 4 pretreatment)
-
Incubation Time:12 h (dose-response); 3-48 h (time-course); 12 h; co-treatment; 1 h pretreatment followed by MD13 treatment
-
Result:Induced dose-dependent MIF degradation, with a half-maximal degradation concentration (DC50) of ~100 nM (western blot) or ~200 nM (ELISA), and maximal degradation of 90-95% at concentrations >1 μM.
Showed a Hook effect at 20 μM.
Induced detectable degradation after 3 h of treatment with 2 μM MD13, reached >92% by 6 h, and showed only slight recovery after 48 h.
Had its MIF degradation effect rescued by co-treatment with Bortezomib, or pretreatment with MIF inhibitor 3 or CRBN inhibitor 4.
-
Cell Line:2D monolayer A549 human lung adenocarcinoma cells
-
Concentration:0.01-20 μM
-
Incubation Time:72 h
-
Result:Inhibited A549 cell proliferation in a dose-dependent manner, with the inhibitory effect visible at nanomolar concentrations and reaching ~50% inhibition at 20 μM.
Showed no significant inhibitory effect from inactive control compounds (MD15, MIF inhibitor 3, CRBN inhibitor 4) at concentrations up to 20 μM.
-
Cell Line:A549 human lung adenocarcinoma cells
-
Concentration:1-5 μM
-
Incubation Time:48 h
-
Result:Induced dose-dependent cell cycle arrest at the G2/M phase in A549 cells.
Increased the proportion of cells in G2/M phase from 12% (control) to 17%, 19%, and 23% with 1, 2, and 5 μM MD13 respectively.
Showed little to no effect on cell cycle distribution from inactive control compound MD15 at 5 μM.
-
Cell Line:A549 human lung adenocarcinoma cells
-
Concentration:2 μM
-
Incubation Time:6-48 h
-
Result:Inhibited ERK phosphorylation in A549 cells by ~50% after 24 h of treatment with 2 μM MD13, with the inhibition persisting at 48 h.
Showed no significant effect on pERK levels from inactive control compounds (MD15, MIF inhibitor 3, CRBN inhibitor 4).
化学情報
-
CAS 番号 2758431-97-7
-
性状 Solid
-
分子量 653.68
-
分子式 C35H35N5O8
-
Color Light yellow to yellow
-
SMILES
O=C(NC1=CC=C(N2C(OC3=CC(O)=CC=C3C2)=O)C=C1)CCCCCCCNC4=CC=CC(C(N5C(CC6)C(NC6=O)=O)=O)=C4C5=O
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
DMSO : 100 mg/mL (152.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
-
データシート (274 KB)
-
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
-
取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5298 mL | 7.6490 mL | 15.2980 mL | 38.2450 mL |
| 5 mM | 0.3060 mL | 1.5298 mL | 3.0596 mL | 7.6490 mL | |
| 10 mM | 0.1530 mL | 0.7649 mL | 1.5298 mL | 3.8245 mL | |
| 15 mM | 0.1020 mL | 0.5099 mL | 1.0199 mL | 2.5497 mL | |
| 20 mM | 0.0765 mL | 0.3825 mL | 0.7649 mL | 1.9123 mL | |
| 25 mM | 0.0612 mL | 0.3060 mL | 0.6119 mL | 1.5298 mL | |
| 30 mM | 0.0510 mL | 0.2550 mL | 0.5099 mL | 1.2748 mL | |
| 40 mM | 0.0382 mL | 0.1912 mL | 0.3825 mL | 0.9561 mL | |
| 50 mM | 0.0306 mL | 0.1530 mL | 0.3060 mL | 0.7649 mL | |
| 60 mM | 0.0255 mL | 0.1275 mL | 0.2550 mL | 0.6374 mL | |
| 80 mM | 0.0191 mL | 0.0956 mL | 0.1912 mL | 0.4781 mL | |
| 100 mM | 0.0153 mL | 0.0765 mL | 0.1530 mL | 0.3825 mL |
MD13 関連分類
HY-148117 関連分類
Powered by Bioz
- MD13
- 2758431-97-7
- MD 13
- MD-13
- PROTACs
- Macrophage migration inhibitory factor (MIF)
- p38 MAPK
- cereblon Cullin RING E3 ubiquitin ligase complex
- MAPK pathway
- MIF
- Macrophage Migration Inhibitory Factor
- 3D tumor spheroid models
- HEK293 cells
- A549 cells
- G2/M cell cycle arrest
- ERK phosphorylation
- lung cancer
- Inhibitor
- inhibitor
- inhibit