Sitamaquine

Sitamaquine (1-30 μM; 15 min) displaces the acidotropic probe Lysotracker Green from acidic organelles of Leishmania donovani L82, Leishmania donovani DD8, Leishmania tropica LCR-L39, and Leishmania tropica SAF-K27 promastigotes, confirming it accumulates in these organelles^[1].
Sitamaquine (5-200 μM; 0-20 min) causes a fast, irreversible reduction in intracellular ATP levels in Leishmania donovani 3-Luc promastigotes, with a 50% reduction observed at concentrations above 30 μM 10 minutes post-treatment^[2].
Sitamaquine (50-100 μM; 15 min) induces mitochondrial membrane depolarization in Leishmania donovani promastigotes, causing a 3.1-fold decrease in Rhodamine 123 accumulation with 100 μM treatment for 15 minutes, and a 3.4-fold decrease in 47% of parasites with 50 μM treatment for 15 minutes^[2].
Sitamaquine (10-200 μM; 1 h) inhibits succinate dehydrogenase activity in a mitochondrion-enriched fraction from Leishmania donovani promastigotes in a dose-dependent manner^[2].
Sitamaquine (50-100 μM; 1 h) induces phosphatidylserine externalization, a marker of apoptosis-like death, in Leishmania donovani promastigotes at 100 μM for 1 hour, causing a 1.9-fold increase in apoptotic cells relative to controls^[2].
Sitamaquine (50-100 μM; 24 h) increases the sub-G₁ DNA population, a marker of chromatin fragmentation, in Leishmania donovani promastigotes^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.